Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach
The aim of this study was to maximize the glucose concentration produced from enzymatic hydrolysis of tapioca starch. The tapioca starch was enzymatically hydrolyzed using α-amylase from Bacillus lichenformis followed by amyloglucosidase action from Aspergillus niger. Effects of liquefaction tempera...
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| Format: | Undergraduates Project Papers |
| Language: | English |
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2009
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| Online Access: | http://umpir.ump.edu.my/id/eprint/863/ http://umpir.ump.edu.my/id/eprint/863/1/Effects%20of%20pH%20and%20temperature%20on%20glucose%20production%20from%20tapioca%20starch%20using%20enzymatic%20hydrolysis%20%20a%20statistical%20approach.pdf |
| _version_ | 1848816728774017024 |
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| author | Siti Nor Shadila, Alias |
| author_facet | Siti Nor Shadila, Alias |
| author_sort | Siti Nor Shadila, Alias |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The aim of this study was to maximize the glucose concentration produced from enzymatic hydrolysis of tapioca starch. The tapioca starch was enzymatically hydrolyzed using α-amylase from Bacillus lichenformis followed by amyloglucosidase action from Aspergillus niger. Effects of liquefaction temperature (X1), saccharification temperature (X2), liquefaction pH (X3) and saccharification pH (X4¬) were evaluated. 24 full factorial design with 1 replicate and 3 centered point was applied to determine the significant parameters affecting the production of glucose concentration. The range of the factors employed were 60 -90˚C (liquefaction temperature), 40-60˚C (saccharification temperature), 5-7 (liquefaction pH) and 4-6 (saccharification pH). The maximum glucose concentration obtained experimentally was 329.10 g/L. The ANOVA shows that the effects of liquefaction temperature and saccharification pH on glucose production were very significant. The saccharification temperature and liquefaction pH, on the other hand did not influence the glucose production. The optimum liquefaction temperature, saccharification temperature, liquefaction pH and saccharification pH suggested by the design of experiment were 90˚C, 60˚C, 7 and 6 respectively. From that optimum condition, the maximum glucose concentration of 331.8 g/L was estimated. |
| first_indexed | 2025-11-15T01:10:29Z |
| format | Undergraduates Project Papers |
| id | ump-863 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:10:29Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-8632023-10-26T00:07:31Z http://umpir.ump.edu.my/id/eprint/863/ Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach Siti Nor Shadila, Alias TP Chemical technology The aim of this study was to maximize the glucose concentration produced from enzymatic hydrolysis of tapioca starch. The tapioca starch was enzymatically hydrolyzed using α-amylase from Bacillus lichenformis followed by amyloglucosidase action from Aspergillus niger. Effects of liquefaction temperature (X1), saccharification temperature (X2), liquefaction pH (X3) and saccharification pH (X4¬) were evaluated. 24 full factorial design with 1 replicate and 3 centered point was applied to determine the significant parameters affecting the production of glucose concentration. The range of the factors employed were 60 -90˚C (liquefaction temperature), 40-60˚C (saccharification temperature), 5-7 (liquefaction pH) and 4-6 (saccharification pH). The maximum glucose concentration obtained experimentally was 329.10 g/L. The ANOVA shows that the effects of liquefaction temperature and saccharification pH on glucose production were very significant. The saccharification temperature and liquefaction pH, on the other hand did not influence the glucose production. The optimum liquefaction temperature, saccharification temperature, liquefaction pH and saccharification pH suggested by the design of experiment were 90˚C, 60˚C, 7 and 6 respectively. From that optimum condition, the maximum glucose concentration of 331.8 g/L was estimated. 2009-05 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/863/1/Effects%20of%20pH%20and%20temperature%20on%20glucose%20production%20from%20tapioca%20starch%20using%20enzymatic%20hydrolysis%20%20a%20statistical%20approach.pdf Siti Nor Shadila, Alias (2009) Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. |
| spellingShingle | TP Chemical technology Siti Nor Shadila, Alias Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach |
| title | Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach |
| title_full | Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach |
| title_fullStr | Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach |
| title_full_unstemmed | Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach |
| title_short | Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach |
| title_sort | effects of ph and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/863/ http://umpir.ump.edu.my/id/eprint/863/1/Effects%20of%20pH%20and%20temperature%20on%20glucose%20production%20from%20tapioca%20starch%20using%20enzymatic%20hydrolysis%20%20a%20statistical%20approach.pdf |