Case Study: Recombinant Bromelain Selection

QR Code

Case Study: Recombinant Bromelain Selection

This chapter presents an investigation that we performed prior to the decision to proceed cloning and producing recombinant bromelain. The criteria that we examined were the following: (1) easy access to a DNA source; (2) broad application; (3) an enzyme size amenable to the cloning strategy; (4) av...

Full description

Bibliographic Details
Main Authors:	Azura, Amid, Nurul Azira, Ismail, Zatul Iffah, Mohd Arshad
Format:	Book Chapter
Published:	Springer International Publishing 2015
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/8240/

Similar Items

Case Study: Recombinant Bromelain Selection
by: Amid, Azura, et al.
Published: (2015)

Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
by: Zatul Iffah, Mohd Arshad, et al.
Published: (2018)

Case Study: Recombinant Bromelain Downstream Processing
by: Amid, Azura, et al.
Published: (2015)

Comparison of Different Cell Disruption Methods And Cell Extractant Buffers For Recombinant Bromelain Expressed in E.COLI BL21-A1
by: Zatul Iffah, Mohd Arshad, et al.
Published: (2015)

Case study: recombinant Bromelain cloning, characterization and upstream processes
by: Amid, Azura, et al.
Published: (2015)

Observation on recombinant bromelain activity before and after purification
by: Ismail, Nurul Azira, et al.
Published: (2010)

Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1
by: Zatul Iffah, Mohd Arshad, et al.
Published: (2017)

Bromelain: an Overview of Industrial Application and Purification Strategies
by: Zatul Iffah, Mohd Arshad, et al.
Published: (2014)

Comparison of cell disruption method for E. coli BL21-AI expressing recombinant bromelain
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)

Functional characterization of recombinant bromelain
by: Amid, Azura, et al.
Published: (2015)

Kinetic studies on recombinant stem bromelain
by: Bala, Muntari, et al.
Published: (2013)

Optimization of spray drying process conditions for recombinant stem bromelain
by: Mohd. Salleh, Hamzah, et al.
Published: (2014)

Comparison of different cell disruption methods and cell extractant buffers for recombinant bromelain expressed in E.coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)

Biosynthesis of nanoparticles using recombinant bromelain
by: Nasruddin, Arina, et al.
Published: (2018)

Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain
by: Ismail, Nurul Azira, et al.
Published: (2011)

Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain
by: Ismail, Nurul Azira, et al.
Published: (2012)

Effects of operating conditions in spray drying of recombinant bromelain
by: Amid, Azura, et al.
Published: (2014)

Effects of process parameters on spray drying of recombinant bromelain
by: Muntari, Bala, et al.
Published: (2013)

Bromelain: an overview of industrial application and purification strategies
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2014)

Expression, purification, and characterization of a recombinant stem bromelain from ananas comosus
by: Amid, Azura, et al.
Published: (2011)

Comparison of purification methods to purify recombinant bromelain from Escherichia coli BL21-A1 = Perbandingan kaedah penulenan rekombinan bromelain daripada Escherichia coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2017)

Bromelain from pineapple: A mini review of its industrial applications and future prospects
by: Thevika, Sarawanan, et al.
Published: (2025)

Rhodopseudomonas palustris collagen-like recombinant protein purification using an aqueous two-phase system
by: Nursyahidatul Azwa, Awang, et al.
Published: (2023)

Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

Recovery of recombinant bromelain from Escherichia coli BL21-AI
by: Bala, Muntari, et al.
Published: (2011)

Pathway analysis of genes affected in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

Optimization on cell disruption of E. coli BL21-AI expressing recombinant bromelain
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2014)

Cytokinetics study on MCF-7 cells treated with commercial and recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2014)

Production of intracellular recombinant bromelain by Escherichia coli BL21-AI using shake flask and stirred tank bioreactor
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2017)

Determination of optimal range of post-induction temperature for production of soluble recombinant bromelain in Escherichia coli using one-factor-at-a-time (OFAT) approach
by: Amid, Azura, et al.
Published: (2011)

Procedure to develop binodal curve and phase diagram for aqueous two-phase system
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2018)

Process optimization on cell disruption of recombinant bromelain expressed in E. coli BL21-AI using ultrasonication
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2013)

Recombinant-enzyme fermentation
by: Amid, Azura, et al.
Published: (2015)

Bromelain production: Current trends and perspective
by: Bala, Muntari, et al.
Published: (2012)

Bromelain enzyme assay using Casen as substrate
by: Amid, Azura, et al.
Published: (2016)

Stability of bromelain-polyphenol complex in pineapple juice
by: Abdul Majid, Fadzilah Adibah, et al.
Published: (2008)

Comparative modelling studies of fruit bromelain using molecular dynamics simulation
by: Pang, Wei Cheng, et al.
Published: (2020)

Heterologous expression of Bromelain in Escherichia coli
by: M., Bala, et al.
Published: (2011)

Molecular cloning sequencing purification of stem bromelain from Ananas comosus
by: Amid, Azura, et al.
Published: (2010)