Bioeconomy: Fermented Waste Management and Pectinases Purification from Thermomyceslanuginosus

Sugar-cane bagasse (SCB) is used for pectinases production from Thermomyceslanuginosus at 55°C under solid-state fermentation (SSF).The study aims to purify total PNL and PL enzymes produced from SCB as the sole carbon source at 55°C using microbial fermentation technology, and to evaluate the ferm...

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Main Authors: Makky, Essam A., M. M., Yusoff
Format: Article
Language:English
Published: Faculty Mechanical Engineering, UMP 2014
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/8237/
http://umpir.ump.edu.my/id/eprint/8237/1/Bioeconomy-_Fermented_Waste_Management_and_Pectinases_Purification_from_Thermomyceslanuginosus.pdf
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author Makky, Essam A.
M. M., Yusoff
author_facet Makky, Essam A.
M. M., Yusoff
author_sort Makky, Essam A.
building UMP Institutional Repository
collection Online Access
description Sugar-cane bagasse (SCB) is used for pectinases production from Thermomyceslanuginosus at 55°C under solid-state fermentation (SSF).The study aims to purify total PNL and PL enzymes produced from SCB as the sole carbon source at 55°C using microbial fermentation technology, and to evaluate the fermented bagasse residuals as fertilizer for the purpose of combatting soil desertification and then detect the amino acids content. The total PNL and PL enzymes were purified and showed two identical peaks, each representing one enzyme. Biotechnological applications of fermented bagasse obtained at 55°C,used as biofertilizer at different concentrations, were cultivated with Zea mays for 30 days to indicate the growth on sandy soil, and induced plant growth which gives an indication of applying the present biofertilizer in reclaimed sandy soils. Only 13 amino acids were detected and were obviously glycine/histidine-containing enzymes. It is apparent that the fermented bagasse successfully improved the sandy soil as biofertilizer and total PNL and PL enzymes were accurately purified at thermophilic conditions under SSF.
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spelling ump-82372018-07-27T03:22:02Z http://umpir.ump.edu.my/id/eprint/8237/ Bioeconomy: Fermented Waste Management and Pectinases Purification from Thermomyceslanuginosus Makky, Essam A. M. M., Yusoff Q Science (General) Sugar-cane bagasse (SCB) is used for pectinases production from Thermomyceslanuginosus at 55°C under solid-state fermentation (SSF).The study aims to purify total PNL and PL enzymes produced from SCB as the sole carbon source at 55°C using microbial fermentation technology, and to evaluate the fermented bagasse residuals as fertilizer for the purpose of combatting soil desertification and then detect the amino acids content. The total PNL and PL enzymes were purified and showed two identical peaks, each representing one enzyme. Biotechnological applications of fermented bagasse obtained at 55°C,used as biofertilizer at different concentrations, were cultivated with Zea mays for 30 days to indicate the growth on sandy soil, and induced plant growth which gives an indication of applying the present biofertilizer in reclaimed sandy soils. Only 13 amino acids were detected and were obviously glycine/histidine-containing enzymes. It is apparent that the fermented bagasse successfully improved the sandy soil as biofertilizer and total PNL and PL enzymes were accurately purified at thermophilic conditions under SSF. Faculty Mechanical Engineering, UMP 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8237/1/Bioeconomy-_Fermented_Waste_Management_and_Pectinases_Purification_from_Thermomyceslanuginosus.pdf Makky, Essam A. and M. M., Yusoff (2014) Bioeconomy: Fermented Waste Management and Pectinases Purification from Thermomyceslanuginosus. Journal of Mechanical Engineering and Sciences (JMES) , 7. pp. 1196-1207. ISSN 2289-4659 (print); 2231-8380 (online). (Published) http://dx.doi.org/10.15282/jmes.7.2014.19.0117 DOI: 10.15282/jmes.7.2014.19.0117
spellingShingle Q Science (General)
Makky, Essam A.
M. M., Yusoff
Bioeconomy: Fermented Waste Management and Pectinases Purification from Thermomyceslanuginosus
title Bioeconomy: Fermented Waste Management and Pectinases Purification from Thermomyceslanuginosus
title_full Bioeconomy: Fermented Waste Management and Pectinases Purification from Thermomyceslanuginosus
title_fullStr Bioeconomy: Fermented Waste Management and Pectinases Purification from Thermomyceslanuginosus
title_full_unstemmed Bioeconomy: Fermented Waste Management and Pectinases Purification from Thermomyceslanuginosus
title_short Bioeconomy: Fermented Waste Management and Pectinases Purification from Thermomyceslanuginosus
title_sort bioeconomy: fermented waste management and pectinases purification from thermomyceslanuginosus
topic Q Science (General)
url http://umpir.ump.edu.my/id/eprint/8237/
http://umpir.ump.edu.my/id/eprint/8237/
http://umpir.ump.edu.my/id/eprint/8237/
http://umpir.ump.edu.my/id/eprint/8237/1/Bioeconomy-_Fermented_Waste_Management_and_Pectinases_Purification_from_Thermomyceslanuginosus.pdf