Growth optimization of N-fixer, isolated from biofertilizer
The objectives of this experiment were to obtain the optimum temperature, pH and the requirement of carbon sources needed for the growth of N-Fixer bacteria. This research can be divided into two parts which are the standard curve preparation followed by optimization of the experiments. Three standa...
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| Format: | Undergraduates Project Papers |
| Language: | English English English English |
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2009
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| Online Access: | http://umpir.ump.edu.my/id/eprint/770/ http://umpir.ump.edu.my/id/eprint/770/1/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28Table%20of%20content%29.pdf http://umpir.ump.edu.my/id/eprint/770/6/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28Abstract%29.pdf http://umpir.ump.edu.my/id/eprint/770/9/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28Chapter%201%29.pdf http://umpir.ump.edu.my/id/eprint/770/16/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28References%29.pdf |
| _version_ | 1848816704520454144 |
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| author | Intan Noorleana, Rosdi |
| author_facet | Intan Noorleana, Rosdi |
| author_sort | Intan Noorleana, Rosdi |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The objectives of this experiment were to obtain the optimum temperature, pH and the requirement of carbon sources needed for the growth of N-Fixer bacteria. This research can be divided into two parts which are the standard curve preparation followed by optimization of the experiments. Three standard curves needs to be constructed; growth, glucose and CFU curves. These three curves will be used in optimizing the growth condition of N-fixer. Set of experimental works were designed by DOE software using full factorial function. Based on the experiment results, it shows that the optimum pH and temperature for the growth of N-Fixer is at pH 6 and 30°C respectively. As for the requirement of carbon sources, 10 g/L was applicable to avoid the surplus glucose become toxic to microorganisms. As the conclusion, the concentration of glucose plays a major role in the fermentation process (18 hours) either to increase the number of N-fixer cell or to decrease it. Therefore, by maintaining it at 10g/L the number of cell can achieve the upper limit to where it can be produced which is about 1.6834E8 cell/mL . |
| first_indexed | 2025-11-15T01:10:06Z |
| format | Undergraduates Project Papers |
| id | ump-770 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English English English |
| last_indexed | 2025-11-15T01:10:06Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-7702021-06-16T08:11:47Z http://umpir.ump.edu.my/id/eprint/770/ Growth optimization of N-fixer, isolated from biofertilizer Intan Noorleana, Rosdi QR Microbiology The objectives of this experiment were to obtain the optimum temperature, pH and the requirement of carbon sources needed for the growth of N-Fixer bacteria. This research can be divided into two parts which are the standard curve preparation followed by optimization of the experiments. Three standard curves needs to be constructed; growth, glucose and CFU curves. These three curves will be used in optimizing the growth condition of N-fixer. Set of experimental works were designed by DOE software using full factorial function. Based on the experiment results, it shows that the optimum pH and temperature for the growth of N-Fixer is at pH 6 and 30°C respectively. As for the requirement of carbon sources, 10 g/L was applicable to avoid the surplus glucose become toxic to microorganisms. As the conclusion, the concentration of glucose plays a major role in the fermentation process (18 hours) either to increase the number of N-fixer cell or to decrease it. Therefore, by maintaining it at 10g/L the number of cell can achieve the upper limit to where it can be produced which is about 1.6834E8 cell/mL . 2009-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/770/1/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28Table%20of%20content%29.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/770/6/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28Abstract%29.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/770/9/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28Chapter%201%29.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/770/16/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28References%29.pdf Intan Noorleana, Rosdi (2009) Growth optimization of N-fixer, isolated from biofertilizer. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. |
| spellingShingle | QR Microbiology Intan Noorleana, Rosdi Growth optimization of N-fixer, isolated from biofertilizer |
| title | Growth optimization of N-fixer, isolated from biofertilizer |
| title_full | Growth optimization of N-fixer, isolated from biofertilizer |
| title_fullStr | Growth optimization of N-fixer, isolated from biofertilizer |
| title_full_unstemmed | Growth optimization of N-fixer, isolated from biofertilizer |
| title_short | Growth optimization of N-fixer, isolated from biofertilizer |
| title_sort | growth optimization of n-fixer, isolated from biofertilizer |
| topic | QR Microbiology |
| url | http://umpir.ump.edu.my/id/eprint/770/ http://umpir.ump.edu.my/id/eprint/770/1/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28Table%20of%20content%29.pdf http://umpir.ump.edu.my/id/eprint/770/6/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28Abstract%29.pdf http://umpir.ump.edu.my/id/eprint/770/9/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28Chapter%201%29.pdf http://umpir.ump.edu.my/id/eprint/770/16/Growth%20optimization%20of%20N-fixer%2C%20isolated%20from%20biofertilizer%20%28References%29.pdf |