Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity

Beta-carotene is used as vitamin A precursor and its antioxidant property has been used in dietary supplements for the prevention of diseases such as cancer and coronary heart disease. The purpose of this study is to study the effect of NaNO3 concentration and light intensity to the growth and produ...

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Main Author: Nur Isma Idayu, Ismail
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/7168/
http://umpir.ump.edu.my/id/eprint/7168/1/Production%20of%20Beta-Carotene%20by%20Dunaliella%20Salina_Effect%20of%20Nano3%20Concentration%20and%20Light%20Intensity.pdf
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author Nur Isma Idayu, Ismail
author_facet Nur Isma Idayu, Ismail
author_sort Nur Isma Idayu, Ismail
building UMP Institutional Repository
collection Online Access
description Beta-carotene is used as vitamin A precursor and its antioxidant property has been used in dietary supplements for the prevention of diseases such as cancer and coronary heart disease. The purpose of this study is to study the effect of NaNO3 concentration and light intensity to the growth and production of beta-carotene by Dunaliella salina. Four different NaNO3 concentrations (100%, 75%, 50%, and 25% v/w) and light intensities (6000 lx, 8000 lx, 11000 lx and 17000 lx) were used. Cell growth was determined by cell counting method and analysis of total beta-carotene concentration in the samples was done using High Performance Liquid Chromatography (HPLC). From the result obtained, the NaNO3 concentration and light intensity will affect the growth of Dunaliella salina, where the growth was reduced due to NaNO3 starvation and it was also induced with the high light intensity until nitrate became the limiting source for the cells. Besides that, NaNO3 concentration and light intensity will also affect the beta-carotene production of Dunaliella salina, where the production was increased as the NaNO3 concentration was decreased and the light intensity was increased until the concentration of NaNO3 has become the limiting factor of the experiment. In order to improve the research, further study to identify the optimal NaNO3 concentration and light intensity required for maximizing the production of beta-carotene by Dunaliella salina shall be done.
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format Undergraduates Project Papers
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institution Universiti Malaysia Pahang
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language English
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publishDate 2012
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spelling ump-71682023-11-09T03:47:59Z http://umpir.ump.edu.my/id/eprint/7168/ Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity Nur Isma Idayu, Ismail QP Physiology Beta-carotene is used as vitamin A precursor and its antioxidant property has been used in dietary supplements for the prevention of diseases such as cancer and coronary heart disease. The purpose of this study is to study the effect of NaNO3 concentration and light intensity to the growth and production of beta-carotene by Dunaliella salina. Four different NaNO3 concentrations (100%, 75%, 50%, and 25% v/w) and light intensities (6000 lx, 8000 lx, 11000 lx and 17000 lx) were used. Cell growth was determined by cell counting method and analysis of total beta-carotene concentration in the samples was done using High Performance Liquid Chromatography (HPLC). From the result obtained, the NaNO3 concentration and light intensity will affect the growth of Dunaliella salina, where the growth was reduced due to NaNO3 starvation and it was also induced with the high light intensity until nitrate became the limiting source for the cells. Besides that, NaNO3 concentration and light intensity will also affect the beta-carotene production of Dunaliella salina, where the production was increased as the NaNO3 concentration was decreased and the light intensity was increased until the concentration of NaNO3 has become the limiting factor of the experiment. In order to improve the research, further study to identify the optimal NaNO3 concentration and light intensity required for maximizing the production of beta-carotene by Dunaliella salina shall be done. 2012-06 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/7168/1/Production%20of%20Beta-Carotene%20by%20Dunaliella%20Salina_Effect%20of%20Nano3%20Concentration%20and%20Light%20Intensity.pdf Nur Isma Idayu, Ismail (2012) Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang.
spellingShingle QP Physiology
Nur Isma Idayu, Ismail
Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity
title Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity
title_full Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity
title_fullStr Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity
title_full_unstemmed Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity
title_short Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity
title_sort production of beta-carotene by dunaliella salina : effect of nano3 concentration and light intensity
topic QP Physiology
url http://umpir.ump.edu.my/id/eprint/7168/
http://umpir.ump.edu.my/id/eprint/7168/1/Production%20of%20Beta-Carotene%20by%20Dunaliella%20Salina_Effect%20of%20Nano3%20Concentration%20and%20Light%20Intensity.pdf