Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer
Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier B.V.
2012
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| Online Access: | http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/1/Targeting_N-acyl-homoserine_lactones_to_mitigate_membrane_biofouling_based_on_quorum_sensing_using_a_biofouling_reducer.pdf http://umpir.ump.edu.my/id/eprint/6817/4/Targeting%20N.pdf |
| _version_ | 1848817863844954112 |
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| author | Muhammad Faisal, Siddiqui Mimi Sakinah, A. M. Singh, Lakhveer Zularisam, A. W. |
| author_facet | Muhammad Faisal, Siddiqui Mimi Sakinah, A. M. Singh, Lakhveer Zularisam, A. W. |
| author_sort | Muhammad Faisal, Siddiqui |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membrane biofouling. This study investigated the role of Piper betle extract (PBE) as an anti-QS agent to mitigate membrane biofouling. Results demonstrated the occurrence of the N-acyl-homoserine-lactone (AHL) autoinducers (AIs), correlate QS activity and membrane biofouling mitigation. The AIs production in bioreactor was confirmed using an indicator strain Agrobacterium tumefaciens (NTL4) harboring plasmid pZLR4. Moreover, three different AHLs were found in biocake using thin layer chromatographic analysis. An increase in extracellular polymeric substances (EPS) and transmembrane pressure (TMP) was observed with AHL activity of the biocake during continuous MBR operation, which shows that membrane biofouling was in close relationship with QS activity. PBE was verified to mitigate membrane biofouling via inhibiting AIs production. SEM analysis further confirmed the effect of PBE on EPS and biofilm formation. These results exhibited that PBE could be a novel agent to target AIs for mitigation of membrane biofouling. Further work can be carried out to purify the active compound of Piper betle extract to target the QS to mitigate membrane biofouling. |
| first_indexed | 2025-11-15T01:28:32Z |
| format | Article |
| id | ump-6817 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T01:28:32Z |
| publishDate | 2012 |
| publisher | Elsevier B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-68172018-01-17T01:05:07Z http://umpir.ump.edu.my/id/eprint/6817/ Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer Muhammad Faisal, Siddiqui Mimi Sakinah, A. M. Singh, Lakhveer Zularisam, A. W. TA Engineering (General). Civil engineering (General) Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membrane biofouling. This study investigated the role of Piper betle extract (PBE) as an anti-QS agent to mitigate membrane biofouling. Results demonstrated the occurrence of the N-acyl-homoserine-lactone (AHL) autoinducers (AIs), correlate QS activity and membrane biofouling mitigation. The AIs production in bioreactor was confirmed using an indicator strain Agrobacterium tumefaciens (NTL4) harboring plasmid pZLR4. Moreover, three different AHLs were found in biocake using thin layer chromatographic analysis. An increase in extracellular polymeric substances (EPS) and transmembrane pressure (TMP) was observed with AHL activity of the biocake during continuous MBR operation, which shows that membrane biofouling was in close relationship with QS activity. PBE was verified to mitigate membrane biofouling via inhibiting AIs production. SEM analysis further confirmed the effect of PBE on EPS and biofilm formation. These results exhibited that PBE could be a novel agent to target AIs for mitigation of membrane biofouling. Further work can be carried out to purify the active compound of Piper betle extract to target the QS to mitigate membrane biofouling. Elsevier B.V. 2012 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6817/1/Targeting_N-acyl-homoserine_lactones_to_mitigate_membrane_biofouling_based_on_quorum_sensing_using_a_biofouling_reducer.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/6817/4/Targeting%20N.pdf Muhammad Faisal, Siddiqui and Mimi Sakinah, A. M. and Singh, Lakhveer and Zularisam, A. W. (2012) Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer. Journal of Biotechnology, 161. pp. 190-197. ISSN 0168-1656. (Published) http://dx.doi.org/10.1016/j.jbiotec.2012.06.029 doi: 10.1016/j.jbiotec.2012.06.029 |
| spellingShingle | TA Engineering (General). Civil engineering (General) Muhammad Faisal, Siddiqui Mimi Sakinah, A. M. Singh, Lakhveer Zularisam, A. W. Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer |
| title | Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer |
| title_full | Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer |
| title_fullStr | Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer |
| title_full_unstemmed | Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer |
| title_short | Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer |
| title_sort | targeting n-acyl-homoserine-lactones to mitigate membrane biofouling based on quorum sensing using a biofouling reducer |
| topic | TA Engineering (General). Civil engineering (General) |
| url | http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/1/Targeting_N-acyl-homoserine_lactones_to_mitigate_membrane_biofouling_based_on_quorum_sensing_using_a_biofouling_reducer.pdf http://umpir.ump.edu.my/id/eprint/6817/4/Targeting%20N.pdf |