Automatic Cryptosporidium And Giardia Viability Detection in Treated Water

In the automatic detection of Cryptosporidium and Giardia (oo)cysts in water samples, low contrast and noise in the microscopic images can adversely affect the accuracy of the segmentation results. An improved partial differential equation (PDE) filtering that achieves a better trade-off between noi...

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Main Authors: Shahriar, Badsha, Norrima, Mokhtar, Hamzah, Arof, Ai Lian Lim, Yvonne, Marizan, Mubin, Zuwairie, Ibrahim
Format: Article
Language:English
Published: SpringerOpen 2013
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/6569/
http://umpir.ump.edu.my/id/eprint/6569/1/Automatic_Cryptosporidium_And_Giardia_Viability_Detection_In_Treated_Water.pdf
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author Shahriar, Badsha
Norrima, Mokhtar
Hamzah, Arof
Ai Lian Lim, Yvonne
Marizan, Mubin
Zuwairie, Ibrahim
author_facet Shahriar, Badsha
Norrima, Mokhtar
Hamzah, Arof
Ai Lian Lim, Yvonne
Marizan, Mubin
Zuwairie, Ibrahim
author_sort Shahriar, Badsha
building UMP Institutional Repository
collection Online Access
description In the automatic detection of Cryptosporidium and Giardia (oo)cysts in water samples, low contrast and noise in the microscopic images can adversely affect the accuracy of the segmentation results. An improved partial differential equation (PDE) filtering that achieves a better trade-off between noise removal and edge preservation is introduced where the compass operator is utilized to attenuate noise while retaining edge information at the cytoplasm wall and around the nuclei of the (oo)cysts. Then the anatomically important information is separated from the unwanted background noise using the Otsu method to improve the detection accuracy. Once the (oo)cysts are located, a simple technique to classify the two types of protozoans using area, roundness metric and eccentricity is implemented. Finally, the number of nuclei in the cytoplasm of each (oo)cyst is counted to check the viability of individual parasite. The proposed system is tested on 40 microscopic images obtained from treated water samples, and it gives excellent detection and viability rates of 97% and 98%, respectively.
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spelling ump-65692018-02-08T02:56:33Z http://umpir.ump.edu.my/id/eprint/6569/ Automatic Cryptosporidium And Giardia Viability Detection in Treated Water Shahriar, Badsha Norrima, Mokhtar Hamzah, Arof Ai Lian Lim, Yvonne Marizan, Mubin Zuwairie, Ibrahim TK Electrical engineering. Electronics Nuclear engineering In the automatic detection of Cryptosporidium and Giardia (oo)cysts in water samples, low contrast and noise in the microscopic images can adversely affect the accuracy of the segmentation results. An improved partial differential equation (PDE) filtering that achieves a better trade-off between noise removal and edge preservation is introduced where the compass operator is utilized to attenuate noise while retaining edge information at the cytoplasm wall and around the nuclei of the (oo)cysts. Then the anatomically important information is separated from the unwanted background noise using the Otsu method to improve the detection accuracy. Once the (oo)cysts are located, a simple technique to classify the two types of protozoans using area, roundness metric and eccentricity is implemented. Finally, the number of nuclei in the cytoplasm of each (oo)cyst is counted to check the viability of individual parasite. The proposed system is tested on 40 microscopic images obtained from treated water samples, and it gives excellent detection and viability rates of 97% and 98%, respectively. SpringerOpen 2013 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/6569/1/Automatic_Cryptosporidium_And_Giardia_Viability_Detection_In_Treated_Water.pdf Shahriar, Badsha and Norrima, Mokhtar and Hamzah, Arof and Ai Lian Lim, Yvonne and Marizan, Mubin and Zuwairie, Ibrahim (2013) Automatic Cryptosporidium And Giardia Viability Detection in Treated Water. EURASIP Journal on Image and Video Processing, 56. pp. 1-10. ISSN 1687-5281. (Published) http://dx.doi.org/10.1186/1687-5281-2013-56 DOI: 10.1186/1687-5281-2013-56
spellingShingle TK Electrical engineering. Electronics Nuclear engineering
Shahriar, Badsha
Norrima, Mokhtar
Hamzah, Arof
Ai Lian Lim, Yvonne
Marizan, Mubin
Zuwairie, Ibrahim
Automatic Cryptosporidium And Giardia Viability Detection in Treated Water
title Automatic Cryptosporidium And Giardia Viability Detection in Treated Water
title_full Automatic Cryptosporidium And Giardia Viability Detection in Treated Water
title_fullStr Automatic Cryptosporidium And Giardia Viability Detection in Treated Water
title_full_unstemmed Automatic Cryptosporidium And Giardia Viability Detection in Treated Water
title_short Automatic Cryptosporidium And Giardia Viability Detection in Treated Water
title_sort automatic cryptosporidium and giardia viability detection in treated water
topic TK Electrical engineering. Electronics Nuclear engineering
url http://umpir.ump.edu.my/id/eprint/6569/
http://umpir.ump.edu.my/id/eprint/6569/
http://umpir.ump.edu.my/id/eprint/6569/
http://umpir.ump.edu.my/id/eprint/6569/1/Automatic_Cryptosporidium_And_Giardia_Viability_Detection_In_Treated_Water.pdf