Application of Adsorption Chromatography in the Papain Processing
A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow v...
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| Format: | Article |
| Language: | English |
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Global Science and Technology Forum
2014
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| Online Access: | http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/1/Application_of_Adsorption_Chromatography_in_the_Papain_Processing.pdf |
| _version_ | 1848817730404220928 |
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| author | He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin |
| author_facet | He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin |
| author_sort | He, Yuhai |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa· s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice. |
| first_indexed | 2025-11-15T01:26:25Z |
| format | Article |
| id | ump-6260 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:26:25Z |
| publishDate | 2014 |
| publisher | Global Science and Technology Forum |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-62602018-03-26T07:33:42Z http://umpir.ump.edu.my/id/eprint/6260/ Application of Adsorption Chromatography in the Papain Processing He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin TP Chemical technology A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa· s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice. Global Science and Technology Forum 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6260/1/Application_of_Adsorption_Chromatography_in_the_Papain_Processing.pdf He, Yuhai and Syed Mohd Saufi, Tuan Chik and Chong, Fui Chin (2014) Application of Adsorption Chromatography in the Papain Processing. GSTF Journal of Chemical Sciences (JChem), 1 (2). pp. 9-15. ISSN 2339-5079 (Online). (Published) https://www.globalsciencejournals.com/article/10.7603%2Fs40837-014-0002-8 doi: 10.7603/s40837-014-0002-8 |
| spellingShingle | TP Chemical technology He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin Application of Adsorption Chromatography in the Papain Processing |
| title | Application of Adsorption Chromatography in the Papain Processing |
| title_full | Application of Adsorption Chromatography in the Papain Processing |
| title_fullStr | Application of Adsorption Chromatography in the Papain Processing |
| title_full_unstemmed | Application of Adsorption Chromatography in the Papain Processing |
| title_short | Application of Adsorption Chromatography in the Papain Processing |
| title_sort | application of adsorption chromatography in the papain processing |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/1/Application_of_Adsorption_Chromatography_in_the_Papain_Processing.pdf |