Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation
The mixed matrix membrane (MMM) concept is extended, for the first time, to produce a hydrophobic interaction chromatography (HIC) membrane using crushed Phenyl Sepharose™ (GE Healthcare Technologies, Uppsala, Sweden) resin and tested for use in whey protein fractionation. The HIC MMM had static bin...
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| Format: | Article |
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Elsevier Ltd
2013
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| Online Access: | http://umpir.ump.edu.my/id/eprint/6215/ |
| _version_ | 1848817722427703296 |
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| author | Syed Mohd Saufi, Tuan Chik Fee, Conan J. |
| author_facet | Syed Mohd Saufi, Tuan Chik Fee, Conan J. |
| author_sort | Syed Mohd Saufi, Tuan Chik |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The mixed matrix membrane (MMM) concept is extended, for the first time, to produce a hydrophobic interaction chromatography (HIC) membrane using crushed Phenyl Sepharose™ (GE Healthcare Technologies, Uppsala, Sweden) resin and tested for use in whey protein fractionation. The HIC MMM had static binding capacities (membrane volume basis) of 18.419±0.605 mg mL−1 for β-lactoglobulin, 45.850±2.753 mg mL−1 for α-lactalbumin, 41.106±9.004 mg mL−1 for bovine serum albumin and 42.467±4.130 mg mL−1 for lactoferin in individual protein solutions. For flowthrough whey fractionation, the adsorption performance of the HIC MMM was similar to a HiTrap™ Phenyl (GE Healthcare Technologies) HIC column. However, the well-known high processing speeds and inherently low pressure drops of MMM chromatography may offer benefits over a conventional packed bed column. |
| first_indexed | 2025-11-15T01:26:17Z |
| format | Article |
| id | ump-6215 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| last_indexed | 2025-11-15T01:26:17Z |
| publishDate | 2013 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-62152018-04-11T01:57:38Z http://umpir.ump.edu.my/id/eprint/6215/ Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation Syed Mohd Saufi, Tuan Chik Fee, Conan J. TP Chemical technology The mixed matrix membrane (MMM) concept is extended, for the first time, to produce a hydrophobic interaction chromatography (HIC) membrane using crushed Phenyl Sepharose™ (GE Healthcare Technologies, Uppsala, Sweden) resin and tested for use in whey protein fractionation. The HIC MMM had static binding capacities (membrane volume basis) of 18.419±0.605 mg mL−1 for β-lactoglobulin, 45.850±2.753 mg mL−1 for α-lactalbumin, 41.106±9.004 mg mL−1 for bovine serum albumin and 42.467±4.130 mg mL−1 for lactoferin in individual protein solutions. For flowthrough whey fractionation, the adsorption performance of the HIC MMM was similar to a HiTrap™ Phenyl (GE Healthcare Technologies) HIC column. However, the well-known high processing speeds and inherently low pressure drops of MMM chromatography may offer benefits over a conventional packed bed column. Elsevier Ltd 2013 Article PeerReviewed Syed Mohd Saufi, Tuan Chik and Fee, Conan J. (2013) Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation. Journal of Membrane Science, 444. pp. 157-163. ISSN 0376-7388. (Published) http://dx.doi.org/10.1016/j.memsci.2013.05.007 DOI: 10.1016/j.memsci.2013.05.007 |
| spellingShingle | TP Chemical technology Syed Mohd Saufi, Tuan Chik Fee, Conan J. Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation |
| title | Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation |
| title_full | Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation |
| title_fullStr | Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation |
| title_full_unstemmed | Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation |
| title_short | Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation |
| title_sort | mixed matrix membrane chromatography based on hydrophobic interaction for whey protein fractionation |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/6215/ http://umpir.ump.edu.my/id/eprint/6215/ http://umpir.ump.edu.my/id/eprint/6215/ |