Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods
In this present study the antioxidant activity of two edible seaweeds Eucheuma cottonii and Padina sp. were determined. The two extraction methods such as conventional and soxhlet were used to extract secondary metabolites by using methanol as a solvent medium. Total phenolic content of crude seawee...
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| Format: | Article |
| Language: | English |
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Medwell Publishing
2014
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| Online Access: | http://umpir.ump.edu.my/id/eprint/5818/ http://umpir.ump.edu.my/id/eprint/5818/1/141-146.pdf |
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| author | Tam, Siow Foon Lian, Ai Ai Kuppusamy, Palaniselvam M. M., Yusoff Natanamurugaraj, Govindan |
| author_facet | Tam, Siow Foon Lian, Ai Ai Kuppusamy, Palaniselvam M. M., Yusoff Natanamurugaraj, Govindan |
| author_sort | Tam, Siow Foon |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | In this present study the antioxidant activity of two edible seaweeds Eucheuma cottonii and Padina sp. were determined. The two extraction methods such as conventional and soxhlet were used to extract secondary metabolites by using methanol as a solvent medium. Total phenolic content of crude seaweed extracts were analysed by standard method. The different antioxidant assays 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching assays were studied. The DPPH and FRAP assays showed positive correlation with expressed higher total phenolic content of methanolic seaweed extracts. Also, β-carotene bleaching assay lower activity compare with BHT as reference control. IR spectra showed the phenolic related functional groups are present the seaweed extract. It mainly responsible for higher rate of antioxidant activity. The methanolic extracts of Padina sp. showed better radical scavenging and higher phenolic content than Eucheuma cottonii. Also, the soxhlet extraction showed higher yield and better radical scavenging activity compared to conventional method. Moreover, the studies confirmed both seaweeds are an effective candidate for the control the free radical scavenging activity.
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| first_indexed | 2025-11-15T01:25:09Z |
| format | Article |
| id | ump-5818 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:25:09Z |
| publishDate | 2014 |
| publisher | Medwell Publishing |
| recordtype | eprints |
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| spelling | ump-58182018-01-22T01:07:20Z http://umpir.ump.edu.my/id/eprint/5818/ Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods Tam, Siow Foon Lian, Ai Ai Kuppusamy, Palaniselvam M. M., Yusoff Natanamurugaraj, Govindan QD Chemistry In this present study the antioxidant activity of two edible seaweeds Eucheuma cottonii and Padina sp. were determined. The two extraction methods such as conventional and soxhlet were used to extract secondary metabolites by using methanol as a solvent medium. Total phenolic content of crude seaweed extracts were analysed by standard method. The different antioxidant assays 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching assays were studied. The DPPH and FRAP assays showed positive correlation with expressed higher total phenolic content of methanolic seaweed extracts. Also, β-carotene bleaching assay lower activity compare with BHT as reference control. IR spectra showed the phenolic related functional groups are present the seaweed extract. It mainly responsible for higher rate of antioxidant activity. The methanolic extracts of Padina sp. showed better radical scavenging and higher phenolic content than Eucheuma cottonii. Also, the soxhlet extraction showed higher yield and better radical scavenging activity compared to conventional method. Moreover, the studies confirmed both seaweeds are an effective candidate for the control the free radical scavenging activity. Medwell Publishing 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5818/1/141-146.pdf Tam, Siow Foon and Lian, Ai Ai and Kuppusamy, Palaniselvam and M. M., Yusoff and Natanamurugaraj, Govindan (2014) Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods. Research Journal of Applied Sciences, 9 (3). pp. 141-146. ISSN 1815-932X. (Published) http://medwelljournals.com/abstract/?doi=rjasci.2014.141.146 10.3923/rjasci.2014.141.146 |
| spellingShingle | QD Chemistry Tam, Siow Foon Lian, Ai Ai Kuppusamy, Palaniselvam M. M., Yusoff Natanamurugaraj, Govindan Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods |
| title | Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods |
| title_full | Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods |
| title_fullStr | Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods |
| title_full_unstemmed | Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods |
| title_short | Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods |
| title_sort | studies on in vitro antioxidant activity of marine edible seaweed from east coastal region, peninsular malaysia using different extraction methods |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/5818/ http://umpir.ump.edu.my/id/eprint/5818/ http://umpir.ump.edu.my/id/eprint/5818/ http://umpir.ump.edu.my/id/eprint/5818/1/141-146.pdf |