Biosynthesized Gold Nanoparticle Developed as a Tool for Detection of HCG hormone in Pregnant Women Urine Sample

This paper presents a microencapsulation study using whey protein isolate and maltodextrin of polyphenol obtained from Orthosiphon stamineus leaves. Polyphenol content was analysed using ultra-performance liquid chromatography. Higher solid concentration leads to higher solution viscosity, bigger pa...

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Main Authors: Kuppusamy, Palaniselvam, M. M., Yusoff, Gaanty Pragas, Maniam, Natanamurugaraj, Govindan
Format: Article
Language:English
Published: Elsevier 2014
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/5314/
http://umpir.ump.edu.my/id/eprint/5314/1/fkksa-2014-kuppusamy-art.pdf
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author Kuppusamy, Palaniselvam
M. M., Yusoff
Gaanty Pragas, Maniam
Natanamurugaraj, Govindan
author_facet Kuppusamy, Palaniselvam
M. M., Yusoff
Gaanty Pragas, Maniam
Natanamurugaraj, Govindan
author_sort Kuppusamy, Palaniselvam
building UMP Institutional Repository
collection Online Access
description This paper presents a microencapsulation study using whey protein isolate and maltodextrin of polyphenol obtained from Orthosiphon stamineus leaves. Polyphenol content was analysed using ultra-performance liquid chromatography. Higher solid concentration leads to higher solution viscosity, bigger particle size, lower moisture content and less dented surface, which may improve particle flowability. Microencapsulation using a least amount of protein (0.05wt.%) yielded better retention of romarinic acid (82.08%), sinensetin (79.57%) and eupatorin (81.08%) than those with higher protein concentration. Meanwhile, 5.33 wt.% of maltodextrin provide the highest polyphenol retention of rosmarinic acid (82.67%), sinensetin (82.24%) and eupatorin (80.19%). The results suggest that eupatorin and rosmarinic acid are more susceptible to thermal degradation than sinensetin during spray drying. Formulation using 5.33% maltodextrin provide a better preservation of polyphenols compared to other formulations.
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publishDate 2014
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spelling ump-53142018-07-27T01:35:20Z http://umpir.ump.edu.my/id/eprint/5314/ Biosynthesized Gold Nanoparticle Developed as a Tool for Detection of HCG hormone in Pregnant Women Urine Sample Kuppusamy, Palaniselvam M. M., Yusoff Gaanty Pragas, Maniam Natanamurugaraj, Govindan QR Microbiology This paper presents a microencapsulation study using whey protein isolate and maltodextrin of polyphenol obtained from Orthosiphon stamineus leaves. Polyphenol content was analysed using ultra-performance liquid chromatography. Higher solid concentration leads to higher solution viscosity, bigger particle size, lower moisture content and less dented surface, which may improve particle flowability. Microencapsulation using a least amount of protein (0.05wt.%) yielded better retention of romarinic acid (82.08%), sinensetin (79.57%) and eupatorin (81.08%) than those with higher protein concentration. Meanwhile, 5.33 wt.% of maltodextrin provide the highest polyphenol retention of rosmarinic acid (82.67%), sinensetin (82.24%) and eupatorin (80.19%). The results suggest that eupatorin and rosmarinic acid are more susceptible to thermal degradation than sinensetin during spray drying. Formulation using 5.33% maltodextrin provide a better preservation of polyphenols compared to other formulations. Elsevier 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5314/1/fkksa-2014-kuppusamy-art.pdf Kuppusamy, Palaniselvam and M. M., Yusoff and Gaanty Pragas, Maniam and Natanamurugaraj, Govindan (2014) Biosynthesized Gold Nanoparticle Developed as a Tool for Detection of HCG hormone in Pregnant Women Urine Sample. Asian Pacific Journal of Tropical Disease, 4 (3). p. 237. ISSN 2222-1808. (Published) http://dx.doi.org/10.1016/S2222-1808(14)60538-7 DOI: 10.1016/S2222-1808(14)60538-7
spellingShingle QR Microbiology
Kuppusamy, Palaniselvam
M. M., Yusoff
Gaanty Pragas, Maniam
Natanamurugaraj, Govindan
Biosynthesized Gold Nanoparticle Developed as a Tool for Detection of HCG hormone in Pregnant Women Urine Sample
title Biosynthesized Gold Nanoparticle Developed as a Tool for Detection of HCG hormone in Pregnant Women Urine Sample
title_full Biosynthesized Gold Nanoparticle Developed as a Tool for Detection of HCG hormone in Pregnant Women Urine Sample
title_fullStr Biosynthesized Gold Nanoparticle Developed as a Tool for Detection of HCG hormone in Pregnant Women Urine Sample
title_full_unstemmed Biosynthesized Gold Nanoparticle Developed as a Tool for Detection of HCG hormone in Pregnant Women Urine Sample
title_short Biosynthesized Gold Nanoparticle Developed as a Tool for Detection of HCG hormone in Pregnant Women Urine Sample
title_sort biosynthesized gold nanoparticle developed as a tool for detection of hcg hormone in pregnant women urine sample
topic QR Microbiology
url http://umpir.ump.edu.my/id/eprint/5314/
http://umpir.ump.edu.my/id/eprint/5314/
http://umpir.ump.edu.my/id/eprint/5314/
http://umpir.ump.edu.my/id/eprint/5314/1/fkksa-2014-kuppusamy-art.pdf