Bioeconomy: Pectinases Purification and Application of Fermented Waste from Thermomyces Lanuginosus

Malaysia takes a step forward to promote and support the development of sustainable and green technology by adopting and master the technology holistically. Bioeconomy refers to all economic activity derived from scientific and research activity focused on biotechnology. Pectin degradation enzymes a...

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Main Authors: Makky, Essam A., M. M., Yusoff
Format: Article
Language:English
Published: Engineering and Technology Publishing 2015
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/5265/
http://umpir.ump.edu.my/id/eprint/5265/1/JOMB_2015.pdf
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author Makky, Essam A.
M. M., Yusoff
author_facet Makky, Essam A.
M. M., Yusoff
author_sort Makky, Essam A.
building UMP Institutional Repository
collection Online Access
description Malaysia takes a step forward to promote and support the development of sustainable and green technology by adopting and master the technology holistically. Bioeconomy refers to all economic activity derived from scientific and research activity focused on biotechnology. Pectin degradation enzymes are widely used in the industrial processing of fruits and vegetables in order to decrease the viscosity of juices and thus to facilitate extraction, maceration, liquefaction, filtration and clarification processes. Sugar-cane bagasse (SCB) is agrowaste produced in large quantities in our environment used as the substrate for Exo-polygalacturonase (Exo-PG) from Thermomyces lanuginosus at 55°C under solid-state fermentation (SSF) culture. Exo-PG was purified using Gelfiltration chromatography and exhibited only one sharp peak. Biotechnological applications of fermented waste obtained, mixed with the fungal biomass at 55°C as biofertilizer at different concentrations (w/w) were cultivated with Zea mays for 30 days to indicate the plant growth on sandy soil and succeeded to induce the growth which may give an indication of applying the present biofertilizer particularly in the reclaimed sandy soils. Only 14 amino acids were detected and obviously that Exo-PG was typical proline containing enzyme.
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spelling ump-52652018-07-27T03:16:31Z http://umpir.ump.edu.my/id/eprint/5265/ Bioeconomy: Pectinases Purification and Application of Fermented Waste from Thermomyces Lanuginosus Makky, Essam A. M. M., Yusoff QR Microbiology Malaysia takes a step forward to promote and support the development of sustainable and green technology by adopting and master the technology holistically. Bioeconomy refers to all economic activity derived from scientific and research activity focused on biotechnology. Pectin degradation enzymes are widely used in the industrial processing of fruits and vegetables in order to decrease the viscosity of juices and thus to facilitate extraction, maceration, liquefaction, filtration and clarification processes. Sugar-cane bagasse (SCB) is agrowaste produced in large quantities in our environment used as the substrate for Exo-polygalacturonase (Exo-PG) from Thermomyces lanuginosus at 55°C under solid-state fermentation (SSF) culture. Exo-PG was purified using Gelfiltration chromatography and exhibited only one sharp peak. Biotechnological applications of fermented waste obtained, mixed with the fungal biomass at 55°C as biofertilizer at different concentrations (w/w) were cultivated with Zea mays for 30 days to indicate the plant growth on sandy soil and succeeded to induce the growth which may give an indication of applying the present biofertilizer particularly in the reclaimed sandy soils. Only 14 amino acids were detected and obviously that Exo-PG was typical proline containing enzyme. Engineering and Technology Publishing 2015-02 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5265/1/JOMB_2015.pdf Makky, Essam A. and M. M., Yusoff (2015) Bioeconomy: Pectinases Purification and Application of Fermented Waste from Thermomyces Lanuginosus. Journal of Medical and Bioengineering, 4 (1). pp. 76-80. ISSN 2301-3796. (Published) http://www.jomb.org/uploadfile/2014/0320/20140320060037828.pdf DOI: 10.12720/jomb.4.1.76-80
spellingShingle QR Microbiology
Makky, Essam A.
M. M., Yusoff
Bioeconomy: Pectinases Purification and Application of Fermented Waste from Thermomyces Lanuginosus
title Bioeconomy: Pectinases Purification and Application of Fermented Waste from Thermomyces Lanuginosus
title_full Bioeconomy: Pectinases Purification and Application of Fermented Waste from Thermomyces Lanuginosus
title_fullStr Bioeconomy: Pectinases Purification and Application of Fermented Waste from Thermomyces Lanuginosus
title_full_unstemmed Bioeconomy: Pectinases Purification and Application of Fermented Waste from Thermomyces Lanuginosus
title_short Bioeconomy: Pectinases Purification and Application of Fermented Waste from Thermomyces Lanuginosus
title_sort bioeconomy: pectinases purification and application of fermented waste from thermomyces lanuginosus
topic QR Microbiology
url http://umpir.ump.edu.my/id/eprint/5265/
http://umpir.ump.edu.my/id/eprint/5265/
http://umpir.ump.edu.my/id/eprint/5265/
http://umpir.ump.edu.my/id/eprint/5265/1/JOMB_2015.pdf