Enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time

Gaharu, a resinous wood that occurs in trees is belong to Aquilaria genus. It contains more than 12 chemical components that can be extracted that produce aromatic smells. To enhance oil availability and extractability, the effect of enzyme to substrate ratio and pretreatment time were studied. Ther...

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Main Author: Hong, Siau Huey
Format: Undergraduates Project Papers
Language:English
Published: 2008
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/479/
http://umpir.ump.edu.my/id/eprint/479/1/Enzymatic-enhanced%20production%20of%20gaharu%20oil%20-%20effects%20of%20enzyme%20loading%20and%20duration%20time.pdf
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author Hong, Siau Huey
author_facet Hong, Siau Huey
author_sort Hong, Siau Huey
building UMP Institutional Repository
collection Online Access
description Gaharu, a resinous wood that occurs in trees is belong to Aquilaria genus. It contains more than 12 chemical components that can be extracted that produce aromatic smells. To enhance oil availability and extractability, the effect of enzyme to substrate ratio and pretreatment time were studied. There are two main processes involved: enzymatic pre-treatment to break down the cell wall of gaharu, and extraction of the gaharu oil. The gaharu woods undergo drying and grinding processes before enzymatic hydrolysis. Gaharu were enzymatically hydrolyzed using Celluclast 1.5 L and the extraction was carried out using hydro-distillation. The extracted oil were then collected and analyzed by calculating the percentage of gaharu oil yield. Prolonged pretreatment time during enzymatic pretreatment process significantly increased the extraction rate of oil from gaharu. The longest pretreatment time, 9 hours gives the yield of 0.1275%. The higher enzyme to gaharu ratio, the higher yield of extracted oil has been achieved. With the highest ratio, 8 ml enzyme/ 400 g gaharu, the yield of extracted gaharu oil is 0.08375%.
first_indexed 2025-11-15T01:09:02Z
format Undergraduates Project Papers
id ump-479
institution Universiti Malaysia Pahang
institution_category Local University
language English
last_indexed 2025-11-15T01:09:02Z
publishDate 2008
recordtype eprints
repository_type Digital Repository
spelling ump-4792023-11-15T00:10:27Z http://umpir.ump.edu.my/id/eprint/479/ Enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time Hong, Siau Huey TP Chemical technology Gaharu, a resinous wood that occurs in trees is belong to Aquilaria genus. It contains more than 12 chemical components that can be extracted that produce aromatic smells. To enhance oil availability and extractability, the effect of enzyme to substrate ratio and pretreatment time were studied. There are two main processes involved: enzymatic pre-treatment to break down the cell wall of gaharu, and extraction of the gaharu oil. The gaharu woods undergo drying and grinding processes before enzymatic hydrolysis. Gaharu were enzymatically hydrolyzed using Celluclast 1.5 L and the extraction was carried out using hydro-distillation. The extracted oil were then collected and analyzed by calculating the percentage of gaharu oil yield. Prolonged pretreatment time during enzymatic pretreatment process significantly increased the extraction rate of oil from gaharu. The longest pretreatment time, 9 hours gives the yield of 0.1275%. The higher enzyme to gaharu ratio, the higher yield of extracted oil has been achieved. With the highest ratio, 8 ml enzyme/ 400 g gaharu, the yield of extracted gaharu oil is 0.08375%. 2008-05 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/479/1/Enzymatic-enhanced%20production%20of%20gaharu%20oil%20-%20effects%20of%20enzyme%20loading%20and%20duration%20time.pdf Hong, Siau Huey (2008) Enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time. Faculty of Chemical Engineering and Natural Resources, Universiti Malaysia Pahang.
spellingShingle TP Chemical technology
Hong, Siau Huey
Enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time
title Enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time
title_full Enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time
title_fullStr Enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time
title_full_unstemmed Enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time
title_short Enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time
title_sort enzymatic-enhanced production of gaharu oil : effects of enzyme loading and duration time
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/479/
http://umpir.ump.edu.my/id/eprint/479/1/Enzymatic-enhanced%20production%20of%20gaharu%20oil%20-%20effects%20of%20enzyme%20loading%20and%20duration%20time.pdf