Culture of tiger milk mushroom

In this study, tiger milk mushrooms (Lignosus rhinocerus) cultivated on various media and nutrient sources are examined for their growth characteristics, bioactive qualities, and possible uses. The best medium was determined to be PDA agar because of its high carbon content, which offers ideal growt...

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Main Author: Soniya Nair, Suresh
Format: Undergraduates Project Papers
Language:English
Published: 2025
Subjects:
Online Access:https://umpir.ump.edu.my/id/eprint/45718/
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author Soniya Nair, Suresh
author_facet Soniya Nair, Suresh
author_sort Soniya Nair, Suresh
building UMP Institutional Repository
collection Online Access
description In this study, tiger milk mushrooms (Lignosus rhinocerus) cultivated on various media and nutrient sources are examined for their growth characteristics, bioactive qualities, and possible uses. The best medium was determined to be PDA agar because of its high carbon content, which offers ideal growth conditions. The biomass weight peaked after 10 days of incubation in MCM medium but then decreased because of resource depletion. Biomass development was efficiently stimulated by carbon sources such as lactose and glucose, but not by sucrose or Gula Melaka, perhaps because of impurities or phenolic chemicals. The nitrogen sources that promoted biomass growth were meat peptone, ammonium nitrate, and yeast extract; sodium nitrate had no impact. Its potential as a nutraceutical element was highlighted by the polyphenol analysis of the cultivated mushroom extract, which showed high amounts of total phenolic content (70.24±0.46mg/L), total caffeoylquinic acid (81.55±1.41mg/L), and total flavonoids (515±5.00mg/L). At the concentration of 20–40 mg/mL, antioxidant activity, as shown by IC₅₀ values, was effective but less than that of ascorbic acid. Compared to Gram-negative bacteria (Escherichia coli), the extract showed stronger suppression against Gram-positive bacteria (Bacillus subtilis). These results highlight the potential of tiger milk mushrooms as ingredients for functional foods because of their advantageous growth, and antibacterial, and antioxidant qualities.
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format Undergraduates Project Papers
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institution Universiti Malaysia Pahang
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spelling ump-457182025-10-02T07:11:41Z https://umpir.ump.edu.my/id/eprint/45718/ Culture of tiger milk mushroom Soniya Nair, Suresh HD Industries. Land use. Labor T Technology (General) In this study, tiger milk mushrooms (Lignosus rhinocerus) cultivated on various media and nutrient sources are examined for their growth characteristics, bioactive qualities, and possible uses. The best medium was determined to be PDA agar because of its high carbon content, which offers ideal growth conditions. The biomass weight peaked after 10 days of incubation in MCM medium but then decreased because of resource depletion. Biomass development was efficiently stimulated by carbon sources such as lactose and glucose, but not by sucrose or Gula Melaka, perhaps because of impurities or phenolic chemicals. The nitrogen sources that promoted biomass growth were meat peptone, ammonium nitrate, and yeast extract; sodium nitrate had no impact. Its potential as a nutraceutical element was highlighted by the polyphenol analysis of the cultivated mushroom extract, which showed high amounts of total phenolic content (70.24±0.46mg/L), total caffeoylquinic acid (81.55±1.41mg/L), and total flavonoids (515±5.00mg/L). At the concentration of 20–40 mg/mL, antioxidant activity, as shown by IC₅₀ values, was effective but less than that of ascorbic acid. Compared to Gram-negative bacteria (Escherichia coli), the extract showed stronger suppression against Gram-positive bacteria (Bacillus subtilis). These results highlight the potential of tiger milk mushrooms as ingredients for functional foods because of their advantageous growth, and antibacterial, and antioxidant qualities. 2025-01 Undergraduates Project Papers NonPeerReviewed pdf en https://umpir.ump.edu.my/id/eprint/45718/1/Culture%20of%20tiger%20milk%20mushroom.pdf Soniya Nair, Suresh (2025) Culture of tiger milk mushroom. Faculty of Industrial Sciences and Technology, Universti Malaysia Pahang Al-Sultan Abdullah.
spellingShingle HD Industries. Land use. Labor
T Technology (General)
Soniya Nair, Suresh
Culture of tiger milk mushroom
title Culture of tiger milk mushroom
title_full Culture of tiger milk mushroom
title_fullStr Culture of tiger milk mushroom
title_full_unstemmed Culture of tiger milk mushroom
title_short Culture of tiger milk mushroom
title_sort culture of tiger milk mushroom
topic HD Industries. Land use. Labor
T Technology (General)
url https://umpir.ump.edu.my/id/eprint/45718/