The formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application

This research studied the potential therapeutic benefits of formulations made from Lignosus rhinocerus (tiger milk mushroom) and Cordyceps sinensis using the cold extraction method. The purposes of this research were to evaluate the anti-inflammatory and antioxidant properties of these formulations...

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Main Author: Peck, En Ting
Format: Undergraduates Project Papers
Language:English
Published: 2025
Subjects:
Online Access:https://umpir.ump.edu.my/id/eprint/45701/
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author Peck, En Ting
author_facet Peck, En Ting
author_sort Peck, En Ting
building UMP Institutional Repository
collection Online Access
description This research studied the potential therapeutic benefits of formulations made from Lignosus rhinocerus (tiger milk mushroom) and Cordyceps sinensis using the cold extraction method. The purposes of this research were to evaluate the anti-inflammatory and antioxidant properties of these formulations and identify the bioactive compounds responsible for these effects. The formulations were tested for their anti-inflammatory and antioxidant properties through BSA and casein protein assays, as well as DPPH and FRAP tests. FTIR and HPLC analyses were also conducted to identify the compounds present. Among the formulations, Formulation E (2:1 ratio of L. rhinocerus to C. sinensis) showed the best results, with 87.25% and 78.21% inhibition in the BSA and casein protein assays, respectively. It also demonstrated the highest antioxidant activity, achieving an RSA of 80.55% and a FRAP absorbance reading of 0.785 ± 0.037. FTIR detected functional groups like amines and alkenes, however, HPLC failed to identify bioactive compounds such as gallic acid and aspirin-like substances. However, HPLC was successfully identified the presence of cordycepin. The results indicated that Formulation E has the greatest potential for therapeutic use. To improve the research, it is recommended to ensure proper sterility during sample preparation and extraction. Also, include additional tests like antimicrobial and toxicity studies. Enhancing data analysis with advanced statistical methods would further improve the accuracy and reliability of the findings, supporting the development of these formulations for health or pharmaceutical applications.
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format Undergraduates Project Papers
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institution Universiti Malaysia Pahang
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language English
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spelling ump-457012025-10-02T07:13:02Z https://umpir.ump.edu.my/id/eprint/45701/ The formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application Peck, En Ting HD Industries. Land use. Labor T Technology (General) This research studied the potential therapeutic benefits of formulations made from Lignosus rhinocerus (tiger milk mushroom) and Cordyceps sinensis using the cold extraction method. The purposes of this research were to evaluate the anti-inflammatory and antioxidant properties of these formulations and identify the bioactive compounds responsible for these effects. The formulations were tested for their anti-inflammatory and antioxidant properties through BSA and casein protein assays, as well as DPPH and FRAP tests. FTIR and HPLC analyses were also conducted to identify the compounds present. Among the formulations, Formulation E (2:1 ratio of L. rhinocerus to C. sinensis) showed the best results, with 87.25% and 78.21% inhibition in the BSA and casein protein assays, respectively. It also demonstrated the highest antioxidant activity, achieving an RSA of 80.55% and a FRAP absorbance reading of 0.785 ± 0.037. FTIR detected functional groups like amines and alkenes, however, HPLC failed to identify bioactive compounds such as gallic acid and aspirin-like substances. However, HPLC was successfully identified the presence of cordycepin. The results indicated that Formulation E has the greatest potential for therapeutic use. To improve the research, it is recommended to ensure proper sterility during sample preparation and extraction. Also, include additional tests like antimicrobial and toxicity studies. Enhancing data analysis with advanced statistical methods would further improve the accuracy and reliability of the findings, supporting the development of these formulations for health or pharmaceutical applications. 2025-02 Undergraduates Project Papers NonPeerReviewed pdf en https://umpir.ump.edu.my/id/eprint/45701/1/The%20formulation%20and%20effect%20of%20tiger%20milk%20mushroom%20and%20cordyceps%20from%20cold%20extraction%20method%20for%20therapeutic%20application.pdf Peck, En Ting (2025) The formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application. Faculty of Industrial Sciences and Technology, Universti Malaysia Pahang Al-Sultan Abdullah.
spellingShingle HD Industries. Land use. Labor
T Technology (General)
Peck, En Ting
The formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application
title The formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application
title_full The formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application
title_fullStr The formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application
title_full_unstemmed The formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application
title_short The formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application
title_sort formulation and effect of tiger milk mushroom and cordyceps from cold extraction method for therapeutic application
topic HD Industries. Land use. Labor
T Technology (General)
url https://umpir.ump.edu.my/id/eprint/45701/