Whole-genome sequencing of a novel Meyerozyma sp. nov, fungi Isolated from Harumanis Mango, Mangifera indica L. in Malaysia
The study of plant-associated microbiomes, including fruit microbiomes, has been extensively explored. However, the limitations of traditional phenotypic assessments using microscopy and biochemical tests have highlighted the need for more comprehensive methods for microbe identification. Advancemen...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2025
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| Online Access: | https://umpir.ump.edu.my/id/eprint/45671/ |
| _version_ | 1848827480457084928 |
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| author | Muhammad Ikhmal, Rosali Siti Munirah, Musa Siti Khadijah, Kiram Yi, Jasmine Teoh Wen Wei, Siew Shing Hajar Fauzan, Ahmad |
| author_facet | Muhammad Ikhmal, Rosali Siti Munirah, Musa Siti Khadijah, Kiram Yi, Jasmine Teoh Wen Wei, Siew Shing Hajar Fauzan, Ahmad |
| author_sort | Muhammad Ikhmal, Rosali |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The study of plant-associated microbiomes, including fruit microbiomes, has been extensively explored. However, the limitations of traditional phenotypic assessments using microscopy and biochemical tests have highlighted the need for more comprehensive methods for microbe identification. Advancement in high-throughput sequencing technologies helped to shift the focus toward molecular approaches, providing comprehensive analysis on their roles in impacting the environment. In this study, a fungal species was isolated from decayed Harumanis mangoes, and its whole genome was sequenced using the Illumina NovaSeq 6000 platform with short-read protocols. Species identification through BLASTN and phylogenetic tree analysis was performed and confirmed the isolate’s close similarity to Meyerozyma caribbica. Genome assembly revealed a total genome size of Meyerozyma sp. K_B strain was 10,404,240 bp, with GC content of 47.2 %, and 95.4 % completeness based on BUSCO assessment. Gene prediction, and functional annotation revealed a total of 5515 coding genes and 5777 coding sequence regions were predicted, associated with various metabolism pathways particularly carbohydrates biosynthesis such as GH5, GH6, GH7, GH16 and GH18, adaptability in host environment such as stress response and membrane transport, and also secondary metabolite biosynthesis like terpene and NRPS clusters highlighting their diverse function particularly in biocontrol activity. These findings provide valuable genomic insight into the potential of Meyerozyma sp. as a postharvest biocontrol agent for fruit management. |
| first_indexed | 2025-11-15T04:01:23Z |
| format | Article |
| id | ump-45671 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T04:01:23Z |
| publishDate | 2025 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-456712025-09-18T07:18:47Z https://umpir.ump.edu.my/id/eprint/45671/ Whole-genome sequencing of a novel Meyerozyma sp. nov, fungi Isolated from Harumanis Mango, Mangifera indica L. in Malaysia Muhammad Ikhmal, Rosali Siti Munirah, Musa Siti Khadijah, Kiram Yi, Jasmine Teoh Wen Wei, Siew Shing Hajar Fauzan, Ahmad QH Natural history QH301 Biology QK Botany The study of plant-associated microbiomes, including fruit microbiomes, has been extensively explored. However, the limitations of traditional phenotypic assessments using microscopy and biochemical tests have highlighted the need for more comprehensive methods for microbe identification. Advancement in high-throughput sequencing technologies helped to shift the focus toward molecular approaches, providing comprehensive analysis on their roles in impacting the environment. In this study, a fungal species was isolated from decayed Harumanis mangoes, and its whole genome was sequenced using the Illumina NovaSeq 6000 platform with short-read protocols. Species identification through BLASTN and phylogenetic tree analysis was performed and confirmed the isolate’s close similarity to Meyerozyma caribbica. Genome assembly revealed a total genome size of Meyerozyma sp. K_B strain was 10,404,240 bp, with GC content of 47.2 %, and 95.4 % completeness based on BUSCO assessment. Gene prediction, and functional annotation revealed a total of 5515 coding genes and 5777 coding sequence regions were predicted, associated with various metabolism pathways particularly carbohydrates biosynthesis such as GH5, GH6, GH7, GH16 and GH18, adaptability in host environment such as stress response and membrane transport, and also secondary metabolite biosynthesis like terpene and NRPS clusters highlighting their diverse function particularly in biocontrol activity. These findings provide valuable genomic insight into the potential of Meyerozyma sp. as a postharvest biocontrol agent for fruit management. Elsevier 2025 Article PeerReviewed pdf en cc_by_4 https://umpir.ump.edu.my/id/eprint/45671/1/Whole-genome%20sequencing%20of%20a%20novel%20Meyerozyma.pdf Muhammad Ikhmal, Rosali and Siti Munirah, Musa and Siti Khadijah, Kiram and Yi, Jasmine Teoh Wen and Wei, Siew Shing and Hajar Fauzan, Ahmad (2025) Whole-genome sequencing of a novel Meyerozyma sp. nov, fungi Isolated from Harumanis Mango, Mangifera indica L. in Malaysia. The Microbe, 7 (100354). pp. 1-11. ISSN 2950-1946. (Published) https://doi.org/10.1016/j.microb.2025.100354 https://doi.org/10.1016/j.microb.2025.100354 https://doi.org/10.1016/j.microb.2025.100354 |
| spellingShingle | QH Natural history QH301 Biology QK Botany Muhammad Ikhmal, Rosali Siti Munirah, Musa Siti Khadijah, Kiram Yi, Jasmine Teoh Wen Wei, Siew Shing Hajar Fauzan, Ahmad Whole-genome sequencing of a novel Meyerozyma sp. nov, fungi Isolated from Harumanis Mango, Mangifera indica L. in Malaysia |
| title | Whole-genome sequencing of a novel Meyerozyma sp. nov, fungi Isolated from Harumanis Mango, Mangifera indica L. in Malaysia |
| title_full | Whole-genome sequencing of a novel Meyerozyma sp. nov, fungi Isolated from Harumanis Mango, Mangifera indica L. in Malaysia |
| title_fullStr | Whole-genome sequencing of a novel Meyerozyma sp. nov, fungi Isolated from Harumanis Mango, Mangifera indica L. in Malaysia |
| title_full_unstemmed | Whole-genome sequencing of a novel Meyerozyma sp. nov, fungi Isolated from Harumanis Mango, Mangifera indica L. in Malaysia |
| title_short | Whole-genome sequencing of a novel Meyerozyma sp. nov, fungi Isolated from Harumanis Mango, Mangifera indica L. in Malaysia |
| title_sort | whole-genome sequencing of a novel meyerozyma sp. nov, fungi isolated from harumanis mango, mangifera indica l. in malaysia |
| topic | QH Natural history QH301 Biology QK Botany |
| url | https://umpir.ump.edu.my/id/eprint/45671/ https://umpir.ump.edu.my/id/eprint/45671/ https://umpir.ump.edu.my/id/eprint/45671/ |