The characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in Kuantan, Malaysia
Background: Diarrhoeagenic verotoxin producing non-O157 Escherichia coli (VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2022
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| Online Access: | https://umpir.ump.edu.my/id/eprint/45358/ |
| _version_ | 1848827393454637056 |
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| author | Md Fazlul, Karim Khan Rashid, Shah Samiur Aizi Nor Mazila, Ramli Kabir, Muhammad Nomani Mohammad Nazmul Hasan, Maziz |
| author_facet | Md Fazlul, Karim Khan Rashid, Shah Samiur Aizi Nor Mazila, Ramli Kabir, Muhammad Nomani Mohammad Nazmul Hasan, Maziz |
| author_sort | Md Fazlul, Karim Khan |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Background: Diarrhoeagenic verotoxin producing non-O157 Escherichia coli (VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and characterisation of VTEC and their association in causing infectious diarrhoea among Malaysian children. Methods: Standard microbiological techniques identified a total of 137 non-repeated, clinically significant E. coli isolates. Serological assays discerned non-O157 E. coli serogroup, subjected to virulence screen (VT1 and VT2) by a polymerase chain reaction (PCR). Results: Different PCR sets characterised the 49 clinical isolates of sorbitol positive non-O157 E. coli. Twenty-nine isolates harboured verotoxin genes associated with diarrhoea among children (≤ 5 years old). Among the 29 (59.18%) strains of verotoxin producing E. coli* genotypes VT1 and VT2 were detected in 21 (42.85%) and 5 (10.20%) isolates respectively, while both VT1 and VT2 genes were confirmed in 3 (6.12%) isolates. Conclusion: This study evaluates on the prevalence, serological characteristics and antimicrobial susceptibility patterns of VTEC diarrhoea affected children (≤ 5 years old). Besides, the prevalence of verotoxin gene was determined as a root cause of diarrhoea among Malaysian children. |
| first_indexed | 2025-11-15T04:00:00Z |
| format | Article |
| id | ump-45358 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T04:00:00Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-453582025-08-12T06:17:55Z https://umpir.ump.edu.my/id/eprint/45358/ The characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in Kuantan, Malaysia Md Fazlul, Karim Khan Rashid, Shah Samiur Aizi Nor Mazila, Ramli Kabir, Muhammad Nomani Mohammad Nazmul Hasan, Maziz QR Microbiology TP Chemical technology Background: Diarrhoeagenic verotoxin producing non-O157 Escherichia coli (VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and characterisation of VTEC and their association in causing infectious diarrhoea among Malaysian children. Methods: Standard microbiological techniques identified a total of 137 non-repeated, clinically significant E. coli isolates. Serological assays discerned non-O157 E. coli serogroup, subjected to virulence screen (VT1 and VT2) by a polymerase chain reaction (PCR). Results: Different PCR sets characterised the 49 clinical isolates of sorbitol positive non-O157 E. coli. Twenty-nine isolates harboured verotoxin genes associated with diarrhoea among children (≤ 5 years old). Among the 29 (59.18%) strains of verotoxin producing E. coli* genotypes VT1 and VT2 were detected in 21 (42.85%) and 5 (10.20%) isolates respectively, while both VT1 and VT2 genes were confirmed in 3 (6.12%) isolates. Conclusion: This study evaluates on the prevalence, serological characteristics and antimicrobial susceptibility patterns of VTEC diarrhoea affected children (≤ 5 years old). Besides, the prevalence of verotoxin gene was determined as a root cause of diarrhoea among Malaysian children. Penerbit Universiti Sains Malaysia 2022 Article PeerReviewed pdf en cc_by_4 https://umpir.ump.edu.my/id/eprint/45358/1/The%20characterisation%20of%20diarrhoeagenic%20verotoxin%20producing%20non-0157.pdf Md Fazlul, Karim Khan and Rashid, Shah Samiur and Aizi Nor Mazila, Ramli and Kabir, Muhammad Nomani and Mohammad Nazmul Hasan, Maziz (2022) The characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in Kuantan, Malaysia. Malaysian Journal of Medical Sciences, 29 (2). pp. 55-68. ISSN 1394-195X. (Published) https://doi.org/10.21315/mjms2022.29.2.6 https://doi.org/10.21315/mjms2022.29.2.6 https://doi.org/10.21315/mjms2022.29.2.6 |
| spellingShingle | QR Microbiology TP Chemical technology Md Fazlul, Karim Khan Rashid, Shah Samiur Aizi Nor Mazila, Ramli Kabir, Muhammad Nomani Mohammad Nazmul Hasan, Maziz The characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in Kuantan, Malaysia |
| title | The characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in Kuantan, Malaysia |
| title_full | The characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in Kuantan, Malaysia |
| title_fullStr | The characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in Kuantan, Malaysia |
| title_full_unstemmed | The characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in Kuantan, Malaysia |
| title_short | The characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in Kuantan, Malaysia |
| title_sort | characterisation of diarrhoeagenic verotoxin producing non-0157 escherichia coli among young children in kuantan, malaysia |
| topic | QR Microbiology TP Chemical technology |
| url | https://umpir.ump.edu.my/id/eprint/45358/ https://umpir.ump.edu.my/id/eprint/45358/ https://umpir.ump.edu.my/id/eprint/45358/ |