Improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic Swiss albino rats by Piper betle juice: evidences from in vivo and in silico studies
Piper betle L. leaves are very popular and traditionally used to chew with betel nut in many Asian countries. In this study, P. betle leaves juice (PBJ) was subjected to evaluation for its antihyperlipidemic activity in the high-fat-diet-induced hyperlipidemic rats model. Swiss albino rats were allo...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cellular and Molecular Biology Association
2022
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| Online Access: | https://umpir.ump.edu.my/id/eprint/45280/ |
| _version_ | 1848827373481361408 |
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| author | Jing, XiBo Sarker, Md. Moklesur Rahman Gifari, Md. Abu Jar Maruf, Md. Rifat Alam Alam, Safaet Khan, Farzana Soma, Mahfuza Afroz Hossain, Md Sanower Ming, Long Chiau Ahmad, Shihab Uddin Hasan, Raquibul |
| author_facet | Jing, XiBo Sarker, Md. Moklesur Rahman Gifari, Md. Abu Jar Maruf, Md. Rifat Alam Alam, Safaet Khan, Farzana Soma, Mahfuza Afroz Hossain, Md Sanower Ming, Long Chiau Ahmad, Shihab Uddin Hasan, Raquibul |
| author_sort | Jing, XiBo |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Piper betle L. leaves are very popular and traditionally used to chew with betel nut in many Asian countries. In this study, P. betle leaves juice (PBJ) was subjected to evaluation for its antihyperlipidemic activity in the high-fat-diet-induced hyperlipidemic rats model. Swiss albino rats were allowed to high-fat- diet for one month, followed by concurrent administration of PBJ for another month. The rats were then sacrificed and collected blood, tissues and organs. Pharmacokinetic, toxicological studies and molecular docking studies were performed using SwissADME, admetSAR and schrodinger suit-2017. Our investigation showed a promising effect of PBJ on body weight, lipid profile, oxidative and antioxidative enzymes, and the principle enzyme responsible for the synthesis of cholesterol. PBJ at 0.5 - 3.0 mL/rat significantly reduced body weight of hyperlipidemic rats compared to control. PBJ at the doses of 1.0, 1.5, 2.0, and 3.0 mL/rat significantly (p<0.05, p<0.01, p<0.001) improved the levels of TC, LDL-c, TG, HDL-c and VLDL-c. Similarly, PBJ doses starting from 1.0 mL/rat to 3.0 mL/rat reduced the oxidative biomarkers AST, ALT, ALP, and creatinine. The level of HMG-CoA was significantly reduced by PBJ doses 1.5, 2, and 3 ml/rat. A number of compounds have been found to have good pharmacokinetic profile and safety and 4-coumaroylquinic acid exerted the best docking score among them. Thus our findings clearly demonstrated the potential lipid-lowering activities of PBJ both in vivo and in silico studies. PBJ can be a good candidate for the development of antihyperlipidemic medication or as an alternative medicine. |
| first_indexed | 2025-11-15T03:59:41Z |
| format | Article |
| id | ump-45280 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:59:41Z |
| publishDate | 2022 |
| publisher | Cellular and Molecular Biology Association |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-452802025-08-07T02:02:10Z https://umpir.ump.edu.my/id/eprint/45280/ Improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic Swiss albino rats by Piper betle juice: evidences from in vivo and in silico studies Jing, XiBo Sarker, Md. Moklesur Rahman Gifari, Md. Abu Jar Maruf, Md. Rifat Alam Alam, Safaet Khan, Farzana Soma, Mahfuza Afroz Hossain, Md Sanower Ming, Long Chiau Ahmad, Shihab Uddin Hasan, Raquibul QK Botany RM Therapeutics. Pharmacology Piper betle L. leaves are very popular and traditionally used to chew with betel nut in many Asian countries. In this study, P. betle leaves juice (PBJ) was subjected to evaluation for its antihyperlipidemic activity in the high-fat-diet-induced hyperlipidemic rats model. Swiss albino rats were allowed to high-fat- diet for one month, followed by concurrent administration of PBJ for another month. The rats were then sacrificed and collected blood, tissues and organs. Pharmacokinetic, toxicological studies and molecular docking studies were performed using SwissADME, admetSAR and schrodinger suit-2017. Our investigation showed a promising effect of PBJ on body weight, lipid profile, oxidative and antioxidative enzymes, and the principle enzyme responsible for the synthesis of cholesterol. PBJ at 0.5 - 3.0 mL/rat significantly reduced body weight of hyperlipidemic rats compared to control. PBJ at the doses of 1.0, 1.5, 2.0, and 3.0 mL/rat significantly (p<0.05, p<0.01, p<0.001) improved the levels of TC, LDL-c, TG, HDL-c and VLDL-c. Similarly, PBJ doses starting from 1.0 mL/rat to 3.0 mL/rat reduced the oxidative biomarkers AST, ALT, ALP, and creatinine. The level of HMG-CoA was significantly reduced by PBJ doses 1.5, 2, and 3 ml/rat. A number of compounds have been found to have good pharmacokinetic profile and safety and 4-coumaroylquinic acid exerted the best docking score among them. Thus our findings clearly demonstrated the potential lipid-lowering activities of PBJ both in vivo and in silico studies. PBJ can be a good candidate for the development of antihyperlipidemic medication or as an alternative medicine. Cellular and Molecular Biology Association 2022 Article PeerReviewed pdf en cc_by_nc_nd_4 https://umpir.ump.edu.my/id/eprint/45280/1/Improvement%20of%20lipid%20profile%20and%20hepatic%20oxidative%20stress.pdf Jing, XiBo and Sarker, Md. Moklesur Rahman and Gifari, Md. Abu Jar and Maruf, Md. Rifat Alam and Alam, Safaet and Khan, Farzana and Soma, Mahfuza Afroz and Hossain, Md Sanower and Ming, Long Chiau and Ahmad, Shihab Uddin and Hasan, Raquibul (2022) Improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic Swiss albino rats by Piper betle juice: evidences from in vivo and in silico studies. Cellular and Molecular Biology, 68 (9). pp. 1-13. ISSN 0145-5680. (Published) https://doi.org/10.14715/cmb/2022.68.9.1 https://doi.org/10.14715/cmb/2022.68.9.1 https://doi.org/10.14715/cmb/2022.68.9.1 |
| spellingShingle | QK Botany RM Therapeutics. Pharmacology Jing, XiBo Sarker, Md. Moklesur Rahman Gifari, Md. Abu Jar Maruf, Md. Rifat Alam Alam, Safaet Khan, Farzana Soma, Mahfuza Afroz Hossain, Md Sanower Ming, Long Chiau Ahmad, Shihab Uddin Hasan, Raquibul Improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic Swiss albino rats by Piper betle juice: evidences from in vivo and in silico studies |
| title | Improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic Swiss albino rats by Piper betle juice: evidences from in vivo and in silico studies |
| title_full | Improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic Swiss albino rats by Piper betle juice: evidences from in vivo and in silico studies |
| title_fullStr | Improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic Swiss albino rats by Piper betle juice: evidences from in vivo and in silico studies |
| title_full_unstemmed | Improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic Swiss albino rats by Piper betle juice: evidences from in vivo and in silico studies |
| title_short | Improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic Swiss albino rats by Piper betle juice: evidences from in vivo and in silico studies |
| title_sort | improvement of lipid profile and hepatic oxidative stress in high-fat-diet- induced hyperlipidemic swiss albino rats by piper betle juice: evidences from in vivo and in silico studies |
| topic | QK Botany RM Therapeutics. Pharmacology |
| url | https://umpir.ump.edu.my/id/eprint/45280/ https://umpir.ump.edu.my/id/eprint/45280/ https://umpir.ump.edu.my/id/eprint/45280/ |