| Summary: | Research on green synthesis methods for silver nanoparticles has significantly risen due to its positive features, such as cost -effectiveness, rapidity, and reduced environmental impact. This study employed an edible bird's nest (EBN), an important economic resource, as the main material for manufacturing silver nanoparticles, utilizing water as the sole solvent. The EBN extract was subjected to metabolite profiling using LC -QTOF-MS in positive mode (ESI+), which identified the presence of lipids, glycosides, peptides, polysaccharides, and disaccharides. The effective production of AgNPs was evidenced by noticeable color changes from translucent to brown upon the addition of silver nitrate to the aqueous EBN extract. Further analysis of these silver nanoparticles was conducted using UV-visible spectroscopy, which identified an absorption peak at 421 nm. Additional characterization was conducted via FESEM, ATR -FTIR spectroscopy, and EDX analysis. The confirmation of the reduction of silver particles was established by the participation of phenolic agents, proteins, and amino acids. The produced nanoparticles had a spherical morphology, with a particle size ranging from 10 to 20 nm. The existence of elemental silver was verified by a prominent and powerful peak at around 3 kilo electron volts (keV) in the energy - dispersive X-ray (EDX) spectrum. The antibacterial efficacy of silver nanoparticles against Escherichia coli and Staphylococcus aureus was assessed using the agar diffusion method to determine their potential.
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