Whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (Mangifera Indica L.)
Microbial communities are present in various environments, influencing their hosts through both advantageous and pathogenic interactions. The advent of next-generation sequencing technologies has enabled more comprehensive exploration of these complex communities. Penicillium georgiense (P. georgien...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Penerbit Universiti Malaysia Pahang
2023
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/44189/ http://umpir.ump.edu.my/id/eprint/44189/1/11741 http://umpir.ump.edu.my/id/eprint/44189/2/Whole_Genome%20Sequencing%20of%20Penicillium%20georgiense.pdf |
| _version_ | 1848827053941456896 |
|---|---|
| author | Hajar Fauzan, Ahmad M. S. I., Sahran Teoh, Jasmine Wen Yi Siti Khadijah, Kiram Jeyakirtana, Jayabalan Muhammad Ikhmal, Rosali Siti Munirah, Musa |
| author_facet | Hajar Fauzan, Ahmad M. S. I., Sahran Teoh, Jasmine Wen Yi Siti Khadijah, Kiram Jeyakirtana, Jayabalan Muhammad Ikhmal, Rosali Siti Munirah, Musa |
| author_sort | Hajar Fauzan, Ahmad |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Microbial communities are present in various environments, influencing their hosts through both advantageous and pathogenic interactions. The advent of next-generation sequencing technologies has enabled more comprehensive exploration of these complex communities. Penicillium georgiense (P. georgiense), a known pathogenic fungus, has been shown to cause significant spoilage through rapid colonization enabled by spore production, particularly affecting long-term storage. Contamination by Penicillium species poses risks to crops health, emphasizing the need for effective post-harvest management. In this study, Harumanis mango, widely consumed in Asia, especially in Malaysia, was selected as the model host. A single colony of P. georgiense was isolated, and their genomic DNA was extracted. The genome of the P. georgiense H_A strain was sequenced using the Illumina NovaSeq 6000 platform with short-read technology, revealing a genome size of 18,324,307 base pairs and a GC content of 46.2%. BUSCO analysis indicated 98% genome completeness, with high similarity to the P. georgiense SRG7 isolate. The annotated genome has been deposited in the NCBI GenBank database for accessibility. This study provides important genomic insights into P. georgiense, advancing our understanding of its pathogenic mechanisms and offering valuable data for improved post-harvest management of Harumanis mango. |
| first_indexed | 2025-11-15T03:54:36Z |
| format | Article |
| id | ump-44189 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T03:54:36Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Malaysia Pahang |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-441892025-03-24T04:07:06Z http://umpir.ump.edu.my/id/eprint/44189/ Whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (Mangifera Indica L.) Hajar Fauzan, Ahmad M. S. I., Sahran Teoh, Jasmine Wen Yi Siti Khadijah, Kiram Jeyakirtana, Jayabalan Muhammad Ikhmal, Rosali Siti Munirah, Musa Q Science (General) QK Botany Microbial communities are present in various environments, influencing their hosts through both advantageous and pathogenic interactions. The advent of next-generation sequencing technologies has enabled more comprehensive exploration of these complex communities. Penicillium georgiense (P. georgiense), a known pathogenic fungus, has been shown to cause significant spoilage through rapid colonization enabled by spore production, particularly affecting long-term storage. Contamination by Penicillium species poses risks to crops health, emphasizing the need for effective post-harvest management. In this study, Harumanis mango, widely consumed in Asia, especially in Malaysia, was selected as the model host. A single colony of P. georgiense was isolated, and their genomic DNA was extracted. The genome of the P. georgiense H_A strain was sequenced using the Illumina NovaSeq 6000 platform with short-read technology, revealing a genome size of 18,324,307 base pairs and a GC content of 46.2%. BUSCO analysis indicated 98% genome completeness, with high similarity to the P. georgiense SRG7 isolate. The annotated genome has been deposited in the NCBI GenBank database for accessibility. This study provides important genomic insights into P. georgiense, advancing our understanding of its pathogenic mechanisms and offering valuable data for improved post-harvest management of Harumanis mango. Penerbit Universiti Malaysia Pahang 2023-12-29 Article PeerReviewed text en http://umpir.ump.edu.my/id/eprint/44189/1/11741 pdf en cc_by_nc_4 http://umpir.ump.edu.my/id/eprint/44189/2/Whole_Genome%20Sequencing%20of%20Penicillium%20georgiense.pdf Hajar Fauzan, Ahmad and M. S. I., Sahran and Teoh, Jasmine Wen Yi and Siti Khadijah, Kiram and Jeyakirtana, Jayabalan and Muhammad Ikhmal, Rosali and Siti Munirah, Musa (2023) Whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (Mangifera Indica L.). Current Science and Technology (CST), 3 (2). pp. 36-41. ISSN 2785-8804. (Published) https://doi.org/10.15282/cst.v3i2.11741 https://doi.org/10.15282/cst.v3i2.11741 |
| spellingShingle | Q Science (General) QK Botany Hajar Fauzan, Ahmad M. S. I., Sahran Teoh, Jasmine Wen Yi Siti Khadijah, Kiram Jeyakirtana, Jayabalan Muhammad Ikhmal, Rosali Siti Munirah, Musa Whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (Mangifera Indica L.) |
| title | Whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (Mangifera Indica L.) |
| title_full | Whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (Mangifera Indica L.) |
| title_fullStr | Whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (Mangifera Indica L.) |
| title_full_unstemmed | Whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (Mangifera Indica L.) |
| title_short | Whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (Mangifera Indica L.) |
| title_sort | whole-genome sequencing of penicillium georgiense, a member of the family trichocomaceae isolated from harumanis mango (mangifera indica l.) |
| topic | Q Science (General) QK Botany |
| url | http://umpir.ump.edu.my/id/eprint/44189/ http://umpir.ump.edu.my/id/eprint/44189/ http://umpir.ump.edu.my/id/eprint/44189/ http://umpir.ump.edu.my/id/eprint/44189/1/11741 http://umpir.ump.edu.my/id/eprint/44189/2/Whole_Genome%20Sequencing%20of%20Penicillium%20georgiense.pdf |