Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence

Indoor air quality is a critical aspect of the built environment, profoundly impacting human health and well-being. The indoor air microbiome, a complex ecosystem comprising bacteria, fungi, viruses, and other microorganisms, plays a pivotal role in indoor air quality. Next-generation sequencing (NG...

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Main Authors: Nor Husna, Mat Hussin, Hajar Fauzan, Ahmad
Format: Conference or Workshop Item
Language:English
Published: AIP Publishing 2025
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/43988/
http://umpir.ump.edu.my/id/eprint/43988/1/Metagenomic%20assessment%20and%20next-generation%20sequencing%20%28NGS%29%20in%20indoor%20air%20microbiome%20analysis.pdf
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author Nor Husna, Mat Hussin
Hajar Fauzan, Ahmad
author_facet Nor Husna, Mat Hussin
Hajar Fauzan, Ahmad
author_sort Nor Husna, Mat Hussin
building UMP Institutional Repository
collection Online Access
description Indoor air quality is a critical aspect of the built environment, profoundly impacting human health and well-being. The indoor air microbiome, a complex ecosystem comprising bacteria, fungi, viruses, and other microorganisms, plays a pivotal role in indoor air quality. Next-generation sequencing (NGS) and metagenomic assessments have revolutionized the study of the indoor air microbiome, enabling researchers to explore microbial diversity and functionality with unprecedented depth and precision. This systematic review aims to provide a comprehensive analysis of the application of NGS and metagenomic techniques in profiling the indoor air microbiome. By synthesizing a vast body of research, the review elucidates the methodology underlying these innovative technologies and examines the diversity of microorganisms in indoor environments by using the preferred reporting items for systematic reviews and meta-analyses (PRISMA) method. Factors influencing microbial composition including geographical location, building design, and human occupancy are explored together with their implications for air quality and human health. Furthermore, the review discusses the implications of indoor air microbiome research for public health and environmental management.
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format Conference or Workshop Item
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institution Universiti Malaysia Pahang
institution_category Local University
language English
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publishDate 2025
publisher AIP Publishing
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spelling ump-439882025-03-11T00:42:44Z http://umpir.ump.edu.my/id/eprint/43988/ Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence Nor Husna, Mat Hussin Hajar Fauzan, Ahmad QR Microbiology QR180 Immunology Indoor air quality is a critical aspect of the built environment, profoundly impacting human health and well-being. The indoor air microbiome, a complex ecosystem comprising bacteria, fungi, viruses, and other microorganisms, plays a pivotal role in indoor air quality. Next-generation sequencing (NGS) and metagenomic assessments have revolutionized the study of the indoor air microbiome, enabling researchers to explore microbial diversity and functionality with unprecedented depth and precision. This systematic review aims to provide a comprehensive analysis of the application of NGS and metagenomic techniques in profiling the indoor air microbiome. By synthesizing a vast body of research, the review elucidates the methodology underlying these innovative technologies and examines the diversity of microorganisms in indoor environments by using the preferred reporting items for systematic reviews and meta-analyses (PRISMA) method. Factors influencing microbial composition including geographical location, building design, and human occupancy are explored together with their implications for air quality and human health. Furthermore, the review discusses the implications of indoor air microbiome research for public health and environmental management. AIP Publishing 2025-01-28 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/43988/1/Metagenomic%20assessment%20and%20next-generation%20sequencing%20%28NGS%29%20in%20indoor%20air%20microbiome%20analysis.pdf Nor Husna, Mat Hussin and Hajar Fauzan, Ahmad (2025) Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence. In: AIP Conference Proceedings. AIP Conference Proceedings; 2nd International Conference on Industry-Academia Initiatives in Biotechnology and Chemistry, iCIABC 2023 , 27 - 28 November 2023 , Virtual, Online. pp. 1-10., 3275 (060002). ISSN 0094-243X ISBN 978-073545107-0 (Published) https://doi.org/10.1063/5.0248780
spellingShingle QR Microbiology
QR180 Immunology
Nor Husna, Mat Hussin
Hajar Fauzan, Ahmad
Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence
title Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence
title_full Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence
title_fullStr Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence
title_full_unstemmed Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence
title_short Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence
title_sort metagenomic assessment and next-generation sequencing (ngs) in indoor air microbiome analysis: a short review of current evidence
topic QR Microbiology
QR180 Immunology
url http://umpir.ump.edu.my/id/eprint/43988/
http://umpir.ump.edu.my/id/eprint/43988/
http://umpir.ump.edu.my/id/eprint/43988/1/Metagenomic%20assessment%20and%20next-generation%20sequencing%20%28NGS%29%20in%20indoor%20air%20microbiome%20analysis.pdf