| Summary: | The study aimed to optimize nitrogen content in biofertilizer production using mushroom waste. Five factors were analyzed using two-level full factorial design (TLFD) including mushroom waste content, reaction time for Bokashi fermentation, agitation of mushroom waste, size of mushroom waste and drying method. The nitrogen content was measured using a HACH Spectrophotometer. The factors were further optimized using a central composite design (CCD) through response surface methodology. The parameters studied included drying temperature and mushroom waste content.
Analysis of variance (ANOVA) showed that the best conditions for producing high nitrogen biofertilizer were a drying temperature of 104 ℃, 70% mushroom waste content, uncut mushroom waste size, a Bokashi fermentation reaction time of 10 days with agitation and nitrogen content of up to 1624 mg/L. The suggested optimum condition for producing high nitrogen biofertilizer was a temperature of 80°C and 70% mushroom waste content, yielding the highest nitrogen content of 1305mg/L. This research suggests mushroom waste as a new alternative for biofertilizer production, adding value-added natural products.
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