Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp
Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier
2024
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| Online Access: | http://umpir.ump.edu.my/id/eprint/42995/ http://umpir.ump.edu.my/id/eprint/42995/1/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp_ABST.pdf http://umpir.ump.edu.my/id/eprint/42995/2/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp.pdf |
| _version_ | 1848826753548550144 |
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| author | Silvanir, . Lai, Sin Yuan Azren Aida, Asmawi Chew, Kit Wayne Ngan, Cheng Loong |
| author_facet | Silvanir, . Lai, Sin Yuan Azren Aida, Asmawi Chew, Kit Wayne Ngan, Cheng Loong |
| author_sort | Silvanir, . |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min. |
| first_indexed | 2025-11-15T03:49:50Z |
| format | Article |
| id | ump-42995 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T03:49:50Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-429952024-12-02T07:07:47Z http://umpir.ump.edu.my/id/eprint/42995/ Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp Silvanir, . Lai, Sin Yuan Azren Aida, Asmawi Chew, Kit Wayne Ngan, Cheng Loong TP Chemical technology Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min. Elsevier 2024-02 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/42995/1/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp_ABST.pdf pdf en http://umpir.ump.edu.my/id/eprint/42995/2/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp.pdf Silvanir, . and Lai, Sin Yuan and Azren Aida, Asmawi and Chew, Kit Wayne and Ngan, Cheng Loong (2024) Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp. Bioresource Technology, 393 (130094). pp. 1-8. ISSN 0960-8524. (Published) https://doi.org/10.1016/j.biortech.2023.130094 https://doi.org/10.1016/j.biortech.2023.130094 |
| spellingShingle | TP Chemical technology Silvanir, . Lai, Sin Yuan Azren Aida, Asmawi Chew, Kit Wayne Ngan, Cheng Loong Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp |
| title | Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp |
| title_full | Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp |
| title_fullStr | Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp |
| title_full_unstemmed | Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp |
| title_short | Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp |
| title_sort | application of high shear-assisted liquid biphasic system for protein extraction from chlorella sp |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/42995/ http://umpir.ump.edu.my/id/eprint/42995/ http://umpir.ump.edu.my/id/eprint/42995/ http://umpir.ump.edu.my/id/eprint/42995/1/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp_ABST.pdf http://umpir.ump.edu.my/id/eprint/42995/2/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp.pdf |