Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock

With the use of biotechnology, hydrogen can be produced from wastewater rich in short-chain fatty acids. A previous study revealed the ability of Rhodobacter sphaeroides KKU-PS1 to produce biohydrogen from substrates mimicking succinate fermentation effluent. However, the process still requires opti...

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Main Authors: Muhammad Alif Fitri, Hanipa, Tiang, Ming Foong, Abdullah Amru, Indera Luthfi, Mohd Shaiful, Sajab, Mimi Hani, Abu Bakar, Reungsang, Alissara, Lay, Chyi-How, Wu, Shu-Yii, Kamrul Fakir, Kamarudin, Peer Mohamed, Abdul
Format: Article
Language:English
English
Published: Elsevier Ltd 2024
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/42963/
http://umpir.ump.edu.my/id/eprint/42963/1/Illumination%20optimization%20strategies%20to%20enhance%20hydrogen%20productivity_ABST.pdf
http://umpir.ump.edu.my/id/eprint/42963/2/Illumination%20optimization%20strategies%20to%20enhance%20hydrogen%20productivity.pdf
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author Muhammad Alif Fitri, Hanipa
Tiang, Ming Foong
Abdullah Amru, Indera Luthfi
Mohd Shaiful, Sajab
Mimi Hani, Abu Bakar
Reungsang, Alissara
Lay, Chyi-How
Wu, Shu-Yii
Kamrul Fakir, Kamarudin
Peer Mohamed, Abdul
author_facet Muhammad Alif Fitri, Hanipa
Tiang, Ming Foong
Abdullah Amru, Indera Luthfi
Mohd Shaiful, Sajab
Mimi Hani, Abu Bakar
Reungsang, Alissara
Lay, Chyi-How
Wu, Shu-Yii
Kamrul Fakir, Kamarudin
Peer Mohamed, Abdul
author_sort Muhammad Alif Fitri, Hanipa
building UMP Institutional Repository
collection Online Access
description With the use of biotechnology, hydrogen can be produced from wastewater rich in short-chain fatty acids. A previous study revealed the ability of Rhodobacter sphaeroides KKU-PS1 to produce biohydrogen from substrates mimicking succinate fermentation effluent. However, the process still requires optimization. Before illumination optimization, due to high concentration of the effluent, various effluent dilution factors ranging from 10 to 100 were compared, and the optimal dilution factor was determined to be 50. Light-emitting diode (LED) setups consisting of bands and tubes were compared, and various illuminated surface-to-volume ratios (S/V) were obtained. LED tubes were subsequently used for light intensity optimization in the range of 5–23 klux, revealing optimum light intensity at 15 klux, yielding 2202 mL H2/L and 13.8 mL H2/L/h as the cumulative hydrogen and maximum output rate, respectively. The lighting protocol at 15 klux and with a 6h–6h light-dark cycle improved the total light conversion efficiency by up to 3.1%. The study successfully optimized the process, with results rivalling those of a previous study using malate.
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institution Universiti Malaysia Pahang
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language English
English
last_indexed 2025-11-15T03:49:43Z
publishDate 2024
publisher Elsevier Ltd
recordtype eprints
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spelling ump-429632024-11-21T07:56:30Z http://umpir.ump.edu.my/id/eprint/42963/ Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock Muhammad Alif Fitri, Hanipa Tiang, Ming Foong Abdullah Amru, Indera Luthfi Mohd Shaiful, Sajab Mimi Hani, Abu Bakar Reungsang, Alissara Lay, Chyi-How Wu, Shu-Yii Kamrul Fakir, Kamarudin Peer Mohamed, Abdul Q Science (General) TP Chemical technology With the use of biotechnology, hydrogen can be produced from wastewater rich in short-chain fatty acids. A previous study revealed the ability of Rhodobacter sphaeroides KKU-PS1 to produce biohydrogen from substrates mimicking succinate fermentation effluent. However, the process still requires optimization. Before illumination optimization, due to high concentration of the effluent, various effluent dilution factors ranging from 10 to 100 were compared, and the optimal dilution factor was determined to be 50. Light-emitting diode (LED) setups consisting of bands and tubes were compared, and various illuminated surface-to-volume ratios (S/V) were obtained. LED tubes were subsequently used for light intensity optimization in the range of 5–23 klux, revealing optimum light intensity at 15 klux, yielding 2202 mL H2/L and 13.8 mL H2/L/h as the cumulative hydrogen and maximum output rate, respectively. The lighting protocol at 15 klux and with a 6h–6h light-dark cycle improved the total light conversion efficiency by up to 3.1%. The study successfully optimized the process, with results rivalling those of a previous study using malate. Elsevier Ltd 2024-10-28 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/42963/1/Illumination%20optimization%20strategies%20to%20enhance%20hydrogen%20productivity_ABST.pdf pdf en http://umpir.ump.edu.my/id/eprint/42963/2/Illumination%20optimization%20strategies%20to%20enhance%20hydrogen%20productivity.pdf Muhammad Alif Fitri, Hanipa and Tiang, Ming Foong and Abdullah Amru, Indera Luthfi and Mohd Shaiful, Sajab and Mimi Hani, Abu Bakar and Reungsang, Alissara and Lay, Chyi-How and Wu, Shu-Yii and Kamrul Fakir, Kamarudin and Peer Mohamed, Abdul (2024) Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock. International Journal of Hydrogen Energy, 88. pp. 418-431. ISSN 0360-3199. (Published) https://doi.org/10.1016/j.ijhydene.2024.09.132 https://doi.org/10.1016/j.ijhydene.2024.09.132
spellingShingle Q Science (General)
TP Chemical technology
Muhammad Alif Fitri, Hanipa
Tiang, Ming Foong
Abdullah Amru, Indera Luthfi
Mohd Shaiful, Sajab
Mimi Hani, Abu Bakar
Reungsang, Alissara
Lay, Chyi-How
Wu, Shu-Yii
Kamrul Fakir, Kamarudin
Peer Mohamed, Abdul
Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock
title Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock
title_full Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock
title_fullStr Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock
title_full_unstemmed Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock
title_short Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock
title_sort illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by rhodobacter sphaeroides kku-ps1 using a concentrated multi-substrate feedstock
topic Q Science (General)
TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/42963/
http://umpir.ump.edu.my/id/eprint/42963/
http://umpir.ump.edu.my/id/eprint/42963/
http://umpir.ump.edu.my/id/eprint/42963/1/Illumination%20optimization%20strategies%20to%20enhance%20hydrogen%20productivity_ABST.pdf
http://umpir.ump.edu.my/id/eprint/42963/2/Illumination%20optimization%20strategies%20to%20enhance%20hydrogen%20productivity.pdf