Endogenous ethanol- and thermo-tolerant strain from Malaysia pineapple peel: Screening and molecular characterisation

The current study aims to identify an endogenous acetic acid bacterial strain isolated from a Malaysian pineapple capable of tolerating high ethanol and temperatures. A total of 23 strains of presumed acetic acid bacteria were isolated from the pineapple peel waste. Subsequently, three isolates with...

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Main Authors: Selvanathan, Yashini K, Nor Hasmaliana, Abdul Manas, Norazwina, Zainol, Nasratun, Masngut
Format: Article
Language:English
English
Published: Elsevier 2024
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/42826/
http://umpir.ump.edu.my/id/eprint/42826/1/Endogenous%20ethanol-%20and%20thermo-tolerant%20strain%20from%20Malaysia%20pineapple%20peel%20Screening.pdf
http://umpir.ump.edu.my/id/eprint/42826/2/Endogenous%20ethanol-%20and%20thermo-tolerant%20strain%20from%20Malaysia%20pineapple%20peel%20Screening%20and%20molecular%20characterisation.pdf
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author Selvanathan, Yashini K
Nor Hasmaliana, Abdul Manas
Norazwina, Zainol
Nasratun, Masngut
author_facet Selvanathan, Yashini K
Nor Hasmaliana, Abdul Manas
Norazwina, Zainol
Nasratun, Masngut
author_sort Selvanathan, Yashini K
building UMP Institutional Repository
collection Online Access
description The current study aims to identify an endogenous acetic acid bacterial strain isolated from a Malaysian pineapple capable of tolerating high ethanol and temperatures. A total of 23 strains of presumed acetic acid bacteria were isolated from the pineapple peel waste. Subsequently, three isolates with the highest acid yield were subjected to 16S rDNA sequencing; Acetobacter tropicalis (A. tropicalis), Staphylococcus haemolyticus, and Staphylococcus ureilyticus. Tolerance evaluations against pH, temperature, ethanol, sugar, and acetic acid demonstrated that all three strains tolerated significant temperatures, ethanol, and sugar levels. Based on the results, A. tropicalis possesses the potential to reduce cooling system load during acetic acid manufacture in hot climate regions with a notable acetic acid yield.
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institution Universiti Malaysia Pahang
institution_category Local University
language English
English
last_indexed 2025-11-15T03:49:09Z
publishDate 2024
publisher Elsevier
recordtype eprints
repository_type Digital Repository
spelling ump-428262024-10-17T07:04:34Z http://umpir.ump.edu.my/id/eprint/42826/ Endogenous ethanol- and thermo-tolerant strain from Malaysia pineapple peel: Screening and molecular characterisation Selvanathan, Yashini K Nor Hasmaliana, Abdul Manas Norazwina, Zainol Nasratun, Masngut HD Industries. Land use. Labor TP Chemical technology The current study aims to identify an endogenous acetic acid bacterial strain isolated from a Malaysian pineapple capable of tolerating high ethanol and temperatures. A total of 23 strains of presumed acetic acid bacteria were isolated from the pineapple peel waste. Subsequently, three isolates with the highest acid yield were subjected to 16S rDNA sequencing; Acetobacter tropicalis (A. tropicalis), Staphylococcus haemolyticus, and Staphylococcus ureilyticus. Tolerance evaluations against pH, temperature, ethanol, sugar, and acetic acid demonstrated that all three strains tolerated significant temperatures, ethanol, and sugar levels. Based on the results, A. tropicalis possesses the potential to reduce cooling system load during acetic acid manufacture in hot climate regions with a notable acetic acid yield. Elsevier 2024 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/42826/1/Endogenous%20ethanol-%20and%20thermo-tolerant%20strain%20from%20Malaysia%20pineapple%20peel%20Screening.pdf pdf en http://umpir.ump.edu.my/id/eprint/42826/2/Endogenous%20ethanol-%20and%20thermo-tolerant%20strain%20from%20Malaysia%20pineapple%20peel%20Screening%20and%20molecular%20characterisation.pdf Selvanathan, Yashini K and Nor Hasmaliana, Abdul Manas and Norazwina, Zainol and Nasratun, Masngut (2024) Endogenous ethanol- and thermo-tolerant strain from Malaysia pineapple peel: Screening and molecular characterisation. Food Bioscience, 62 (105198). pp. 1-9. ISSN 2212-4292. (Published) https://doi.org/10.1016/j.fbio.2024.105198 10.1016/j.fbio.2024.105198
spellingShingle HD Industries. Land use. Labor
TP Chemical technology
Selvanathan, Yashini K
Nor Hasmaliana, Abdul Manas
Norazwina, Zainol
Nasratun, Masngut
Endogenous ethanol- and thermo-tolerant strain from Malaysia pineapple peel: Screening and molecular characterisation
title Endogenous ethanol- and thermo-tolerant strain from Malaysia pineapple peel: Screening and molecular characterisation
title_full Endogenous ethanol- and thermo-tolerant strain from Malaysia pineapple peel: Screening and molecular characterisation
title_fullStr Endogenous ethanol- and thermo-tolerant strain from Malaysia pineapple peel: Screening and molecular characterisation
title_full_unstemmed Endogenous ethanol- and thermo-tolerant strain from Malaysia pineapple peel: Screening and molecular characterisation
title_short Endogenous ethanol- and thermo-tolerant strain from Malaysia pineapple peel: Screening and molecular characterisation
title_sort endogenous ethanol- and thermo-tolerant strain from malaysia pineapple peel: screening and molecular characterisation
topic HD Industries. Land use. Labor
TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/42826/
http://umpir.ump.edu.my/id/eprint/42826/
http://umpir.ump.edu.my/id/eprint/42826/
http://umpir.ump.edu.my/id/eprint/42826/1/Endogenous%20ethanol-%20and%20thermo-tolerant%20strain%20from%20Malaysia%20pineapple%20peel%20Screening.pdf
http://umpir.ump.edu.my/id/eprint/42826/2/Endogenous%20ethanol-%20and%20thermo-tolerant%20strain%20from%20Malaysia%20pineapple%20peel%20Screening%20and%20molecular%20characterisation.pdf