Factorial analysis of xylanase and cellulase production from pineapple peel waste
Pineapple wastes (skin, core and crown) are mainly composed of carbohydrates (cellulose, hemicellulose, and lignin). Non-starch polysaccharides (NSP) in feed are indigestible by the endogenous enzymes in poultry. Thus, exogenous enzymes (xylanase and cellulase) are required to overcome this problem....
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| Format: | Book Chapter |
| Language: | English English |
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Trans Tech Publications Ltd
2022
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| Online Access: | http://umpir.ump.edu.my/id/eprint/42718/ http://umpir.ump.edu.my/id/eprint/42718/1/Factorial%20Analysis%20of%20Xylanase%20and%20Cellulase%20Production%20from%20Pineapple%20Peel%20Waste%20-%20intro.pdf http://umpir.ump.edu.my/id/eprint/42718/2/Factorial%20Analysis%20of%20Xylanase%20and%20Cellulase%20Production%20from%20Pineapple%20Peel%20Waste.pdf |
| _version_ | 1848826684249210880 |
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| author | Sivanesan, Pavethra Zatul Iffah, Mohd Arshad Jun Haslinda, Shariffuddin Nasratun, Masngut Norazwina, Zainol Shalyda, Md Shaarani |
| author_facet | Sivanesan, Pavethra Zatul Iffah, Mohd Arshad Jun Haslinda, Shariffuddin Nasratun, Masngut Norazwina, Zainol Shalyda, Md Shaarani |
| author_sort | Sivanesan, Pavethra |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Pineapple wastes (skin, core and crown) are mainly composed of carbohydrates (cellulose, hemicellulose, and lignin). Non-starch polysaccharides (NSP) in feed are indigestible by the endogenous enzymes in poultry. Thus, exogenous enzymes (xylanase and cellulase) are required to overcome this problem. Due to high fiber content, pineapple wastes are unsuitable for animal feed. However, the fermented waste juice could be used to produce enzymes. The objective of this study is to produce xylanase and cellulase from the fermentation of pineapple waste using Lactobacillus casei (L. casei) bacteria inoculated from probiotic drink. The fermentation was performed using different screening parameters (incubation time, temperature, pH value and substrate concentration) according to Two-Level Full Factorial Design (FFD) by Design Expert. From this study, the incubation temperature and substrate concentration had the highest influence on the xylanase activity (39.82 U/mL) while the fermentation time and substrate concentration mostly affected the cellulase activity (8.05 U/mL). Meanwhile, the pH had the least influence on both enzyme activities. The pineapple waste at its best fermentation parameters not only offers an economical way of high enzyme production but also alleviates the agricultural waste disposal issue. Further optimization of the pineapple waste fermentation parameters is required though to maximize enzyme production. |
| first_indexed | 2025-11-15T03:48:44Z |
| format | Book Chapter |
| id | ump-42718 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T03:48:44Z |
| publishDate | 2022 |
| publisher | Trans Tech Publications Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-427182024-10-02T04:56:09Z http://umpir.ump.edu.my/id/eprint/42718/ Factorial analysis of xylanase and cellulase production from pineapple peel waste Sivanesan, Pavethra Zatul Iffah, Mohd Arshad Jun Haslinda, Shariffuddin Nasratun, Masngut Norazwina, Zainol Shalyda, Md Shaarani TP Chemical technology Pineapple wastes (skin, core and crown) are mainly composed of carbohydrates (cellulose, hemicellulose, and lignin). Non-starch polysaccharides (NSP) in feed are indigestible by the endogenous enzymes in poultry. Thus, exogenous enzymes (xylanase and cellulase) are required to overcome this problem. Due to high fiber content, pineapple wastes are unsuitable for animal feed. However, the fermented waste juice could be used to produce enzymes. The objective of this study is to produce xylanase and cellulase from the fermentation of pineapple waste using Lactobacillus casei (L. casei) bacteria inoculated from probiotic drink. The fermentation was performed using different screening parameters (incubation time, temperature, pH value and substrate concentration) according to Two-Level Full Factorial Design (FFD) by Design Expert. From this study, the incubation temperature and substrate concentration had the highest influence on the xylanase activity (39.82 U/mL) while the fermentation time and substrate concentration mostly affected the cellulase activity (8.05 U/mL). Meanwhile, the pH had the least influence on both enzyme activities. The pineapple waste at its best fermentation parameters not only offers an economical way of high enzyme production but also alleviates the agricultural waste disposal issue. Further optimization of the pineapple waste fermentation parameters is required though to maximize enzyme production. Trans Tech Publications Ltd 2022 Book Chapter PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/42718/1/Factorial%20Analysis%20of%20Xylanase%20and%20Cellulase%20Production%20from%20Pineapple%20Peel%20Waste%20-%20intro.pdf pdf en http://umpir.ump.edu.my/id/eprint/42718/2/Factorial%20Analysis%20of%20Xylanase%20and%20Cellulase%20Production%20from%20Pineapple%20Peel%20Waste.pdf Sivanesan, Pavethra and Zatul Iffah, Mohd Arshad and Jun Haslinda, Shariffuddin and Nasratun, Masngut and Norazwina, Zainol and Shalyda, Md Shaarani (2022) Factorial analysis of xylanase and cellulase production from pineapple peel waste. In: Materials Science Forum. Trans Tech Publications Ltd, Switzerland, pp. 241-253. ISBN 1662-9752 https://doi.org/10.4028/p-4ewmy1 10.4028/p-4ewmy1 |
| spellingShingle | TP Chemical technology Sivanesan, Pavethra Zatul Iffah, Mohd Arshad Jun Haslinda, Shariffuddin Nasratun, Masngut Norazwina, Zainol Shalyda, Md Shaarani Factorial analysis of xylanase and cellulase production from pineapple peel waste |
| title | Factorial analysis of xylanase and cellulase production from pineapple peel waste |
| title_full | Factorial analysis of xylanase and cellulase production from pineapple peel waste |
| title_fullStr | Factorial analysis of xylanase and cellulase production from pineapple peel waste |
| title_full_unstemmed | Factorial analysis of xylanase and cellulase production from pineapple peel waste |
| title_short | Factorial analysis of xylanase and cellulase production from pineapple peel waste |
| title_sort | factorial analysis of xylanase and cellulase production from pineapple peel waste |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/42718/ http://umpir.ump.edu.my/id/eprint/42718/ http://umpir.ump.edu.my/id/eprint/42718/ http://umpir.ump.edu.my/id/eprint/42718/1/Factorial%20Analysis%20of%20Xylanase%20and%20Cellulase%20Production%20from%20Pineapple%20Peel%20Waste%20-%20intro.pdf http://umpir.ump.edu.my/id/eprint/42718/2/Factorial%20Analysis%20of%20Xylanase%20and%20Cellulase%20Production%20from%20Pineapple%20Peel%20Waste.pdf |