Detection of 17-α-Ethinylestradiol with Bio-Functionalized tapered optical fiber sensor

This paper presents the utilization of tapered optical fiber (TOF) biosensor for the detection of 17-α-ethinylestradiol (EE2), an estrogen hormone. TOF with a waist diameter of 12 μm was fabricated from single mode fiber using heat and pull method. The tapered area was used as the sensing interface...

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Main Authors: Rosyati, Hamid, Yasmin, Mustapha Kamil, Ahmad Zaharin, Aris, Muhammad Hafiz, Abu Bakar, Fariza Hanim, Suhailin, Alresheedi, Mohammed Thamer, Ng, Eng Khoon, Mohd Adzir, Mahdi
Format: Article
Language:English
English
Published: Elsevier Ltd 2024
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/42555/
http://umpir.ump.edu.my/id/eprint/42555/1/Detection%20of%2017-%CE%B1-Ethinylestradiol%20with%20Bio-Functionalized%20tapered%20optical%20fiber%20sensor_ABST.pdf
http://umpir.ump.edu.my/id/eprint/42555/2/Detection%20of%2017-%CE%B1-Ethinylestradiol%20with%20Bio-Functionalized%20tapered%20optical%20fiber%20sensor.pdf
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author Rosyati, Hamid
Yasmin, Mustapha Kamil
Ahmad Zaharin, Aris
Muhammad Hafiz, Abu Bakar
Fariza Hanim, Suhailin
Alresheedi, Mohammed Thamer
Ng, Eng Khoon
Mohd Adzir, Mahdi
author_facet Rosyati, Hamid
Yasmin, Mustapha Kamil
Ahmad Zaharin, Aris
Muhammad Hafiz, Abu Bakar
Fariza Hanim, Suhailin
Alresheedi, Mohammed Thamer
Ng, Eng Khoon
Mohd Adzir, Mahdi
author_sort Rosyati, Hamid
building UMP Institutional Repository
collection Online Access
description This paper presents the utilization of tapered optical fiber (TOF) biosensor for the detection of 17-α-ethinylestradiol (EE2), an estrogen hormone. TOF with a waist diameter of 12 μm was fabricated from single mode fiber using heat and pull method. The tapered area was used as the sensing interface between the evanescent waves propagating around the fiber due to its unique geometry and the attached molecules on the tapered surface. EE2 antibodies were used as biorecognition molecules towards the targeted analyte, EE2. When tested with different concentrations of EE2 within the range of 0.1 ng/L – 1 mg/L, the developed sensor attained a detection limit of 1 ng/L with a sensitivity value of 1.088 nm/ngL-1 within the operation range of 1 – 10 ng/L. The specificity of the sensor was validated by testing the developed sensor with estrone (E1) and no significant wavelength shift was observed. These findings highlight the potential of our approach for sensitive and selective detection of EE2.
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institution Universiti Malaysia Pahang
institution_category Local University
language English
English
last_indexed 2025-11-15T03:48:04Z
publishDate 2024
publisher Elsevier Ltd
recordtype eprints
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spelling ump-425552024-09-10T04:36:53Z http://umpir.ump.edu.my/id/eprint/42555/ Detection of 17-α-Ethinylestradiol with Bio-Functionalized tapered optical fiber sensor Rosyati, Hamid Yasmin, Mustapha Kamil Ahmad Zaharin, Aris Muhammad Hafiz, Abu Bakar Fariza Hanim, Suhailin Alresheedi, Mohammed Thamer Ng, Eng Khoon Mohd Adzir, Mahdi TP Chemical technology This paper presents the utilization of tapered optical fiber (TOF) biosensor for the detection of 17-α-ethinylestradiol (EE2), an estrogen hormone. TOF with a waist diameter of 12 μm was fabricated from single mode fiber using heat and pull method. The tapered area was used as the sensing interface between the evanescent waves propagating around the fiber due to its unique geometry and the attached molecules on the tapered surface. EE2 antibodies were used as biorecognition molecules towards the targeted analyte, EE2. When tested with different concentrations of EE2 within the range of 0.1 ng/L – 1 mg/L, the developed sensor attained a detection limit of 1 ng/L with a sensitivity value of 1.088 nm/ngL-1 within the operation range of 1 – 10 ng/L. The specificity of the sensor was validated by testing the developed sensor with estrone (E1) and no significant wavelength shift was observed. These findings highlight the potential of our approach for sensitive and selective detection of EE2. Elsevier Ltd 2024-07-14 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/42555/1/Detection%20of%2017-%CE%B1-Ethinylestradiol%20with%20Bio-Functionalized%20tapered%20optical%20fiber%20sensor_ABST.pdf pdf en http://umpir.ump.edu.my/id/eprint/42555/2/Detection%20of%2017-%CE%B1-Ethinylestradiol%20with%20Bio-Functionalized%20tapered%20optical%20fiber%20sensor.pdf Rosyati, Hamid and Yasmin, Mustapha Kamil and Ahmad Zaharin, Aris and Muhammad Hafiz, Abu Bakar and Fariza Hanim, Suhailin and Alresheedi, Mohammed Thamer and Ng, Eng Khoon and Mohd Adzir, Mahdi (2024) Detection of 17-α-Ethinylestradiol with Bio-Functionalized tapered optical fiber sensor. Measurement, 238 (115305). pp. 1-11. ISSN 0263-2241. (Published) https://doi.org/10.1016/j.measurement.2024.115305 https://doi.org/10.1016/j.measurement.2024.115305
spellingShingle TP Chemical technology
Rosyati, Hamid
Yasmin, Mustapha Kamil
Ahmad Zaharin, Aris
Muhammad Hafiz, Abu Bakar
Fariza Hanim, Suhailin
Alresheedi, Mohammed Thamer
Ng, Eng Khoon
Mohd Adzir, Mahdi
Detection of 17-α-Ethinylestradiol with Bio-Functionalized tapered optical fiber sensor
title Detection of 17-α-Ethinylestradiol with Bio-Functionalized tapered optical fiber sensor
title_full Detection of 17-α-Ethinylestradiol with Bio-Functionalized tapered optical fiber sensor
title_fullStr Detection of 17-α-Ethinylestradiol with Bio-Functionalized tapered optical fiber sensor
title_full_unstemmed Detection of 17-α-Ethinylestradiol with Bio-Functionalized tapered optical fiber sensor
title_short Detection of 17-α-Ethinylestradiol with Bio-Functionalized tapered optical fiber sensor
title_sort detection of 17-α-ethinylestradiol with bio-functionalized tapered optical fiber sensor
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/42555/
http://umpir.ump.edu.my/id/eprint/42555/
http://umpir.ump.edu.my/id/eprint/42555/
http://umpir.ump.edu.my/id/eprint/42555/1/Detection%20of%2017-%CE%B1-Ethinylestradiol%20with%20Bio-Functionalized%20tapered%20optical%20fiber%20sensor_ABST.pdf
http://umpir.ump.edu.my/id/eprint/42555/2/Detection%20of%2017-%CE%B1-Ethinylestradiol%20with%20Bio-Functionalized%20tapered%20optical%20fiber%20sensor.pdf