Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model
Zebrafish is a developing vertebrate model with several advantages, including its small size, and high experimental efficiency. Malaysia exhibit one of the highest diabetes rates in the Western Pacific and incurring an annual cost of 600 million US dollars. The objective of the study is to determine...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier Ltd
2024
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| Online Access: | http://umpir.ump.edu.my/id/eprint/41098/ http://umpir.ump.edu.my/id/eprint/41098/1/Green%20honey%20of%20Banggi%20Island%20-%20A%20preliminary%20anti-diabetic%20study%20on%20zebrafish%20model.pdf |
| _version_ | 1848826236204220416 |
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| author | ullah, Saeed Huyop, Fahrul Nurul Huda, . Roswanira, Abdul Wahab Azzmer Azzar, Abdul Hamid Mohd Azrul Naim, Muhammad Hajar Fauzan, Ahmad Amir Husni, Mohd Shariff Mohd Hamzah, Mohd Nasir |
| author_facet | ullah, Saeed Huyop, Fahrul Nurul Huda, . Roswanira, Abdul Wahab Azzmer Azzar, Abdul Hamid Mohd Azrul Naim, Muhammad Hajar Fauzan, Ahmad Amir Husni, Mohd Shariff Mohd Hamzah, Mohd Nasir |
| author_sort | ullah, Saeed |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Zebrafish is a developing vertebrate model with several advantages, including its small size, and high experimental efficiency. Malaysia exhibit one of the highest diabetes rates in the Western Pacific and incurring an annual cost of 600 million US dollars. The objective of the study is to determine the antidiabetic properties of green honey (GH) using a zebrafish model. Adult zebrafish, aged 3–4 months, were subjected to overfeeding and treated with streptozotocin (STZ) through intraperitoneal injection (IP) on days 7 and 9. The study assessed the oral sucrose tolerance test (OSTT) and the anti-diabetic effects of green honey. The evaluation was conducted at three time points: 30, 60, and 120 min after treatment and sucrose administration. The study utilised a model with a sample size of 5. The study was performed in six groups. These groups are (1) Normal control (non-diabetic, no intervention), (2) Normal control + GH (non-diabetic, supplemented with GH 3 μl), (3) DM control (diabetic, no intervention), (4) DM Gp1 (diabetic, 3 μL GH), (5) DM Gp2 (diabetic, 6 μ L GH), (6) DM Acarbose (diabetic, treated with acarbose). Fasting blood glucose levels for non-diabetic (non-DM) and diabetic (DM) groups were evaluated before and after the 10 days of diabetic induction. DM groups (excess of food and two injections of STZ) have caused a significant increment in the fasting blood glucose to 11.55 mmol/l (p < 0.0001). Both GH treatments effectively decreased postprandial blood glucose levels and the area under the curve in the oral glucose tolerance test (OSTT). Based on these results, it is concluded that green honey could play a role in hyperglycemia management and show potential as a natural alternative to conventional diabetes therapy. The underlying mechanisms need to be clarified, and their potential use in human diabetes therapy needs to be investigated. |
| first_indexed | 2025-11-15T03:41:36Z |
| format | Article |
| id | ump-41098 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:41:36Z |
| publishDate | 2024 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-410982024-05-03T05:58:47Z http://umpir.ump.edu.my/id/eprint/41098/ Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model ullah, Saeed Huyop, Fahrul Nurul Huda, . Roswanira, Abdul Wahab Azzmer Azzar, Abdul Hamid Mohd Azrul Naim, Muhammad Hajar Fauzan, Ahmad Amir Husni, Mohd Shariff Mohd Hamzah, Mohd Nasir HD28 Management. Industrial Management Zebrafish is a developing vertebrate model with several advantages, including its small size, and high experimental efficiency. Malaysia exhibit one of the highest diabetes rates in the Western Pacific and incurring an annual cost of 600 million US dollars. The objective of the study is to determine the antidiabetic properties of green honey (GH) using a zebrafish model. Adult zebrafish, aged 3–4 months, were subjected to overfeeding and treated with streptozotocin (STZ) through intraperitoneal injection (IP) on days 7 and 9. The study assessed the oral sucrose tolerance test (OSTT) and the anti-diabetic effects of green honey. The evaluation was conducted at three time points: 30, 60, and 120 min after treatment and sucrose administration. The study utilised a model with a sample size of 5. The study was performed in six groups. These groups are (1) Normal control (non-diabetic, no intervention), (2) Normal control + GH (non-diabetic, supplemented with GH 3 μl), (3) DM control (diabetic, no intervention), (4) DM Gp1 (diabetic, 3 μL GH), (5) DM Gp2 (diabetic, 6 μ L GH), (6) DM Acarbose (diabetic, treated with acarbose). Fasting blood glucose levels for non-diabetic (non-DM) and diabetic (DM) groups were evaluated before and after the 10 days of diabetic induction. DM groups (excess of food and two injections of STZ) have caused a significant increment in the fasting blood glucose to 11.55 mmol/l (p < 0.0001). Both GH treatments effectively decreased postprandial blood glucose levels and the area under the curve in the oral glucose tolerance test (OSTT). Based on these results, it is concluded that green honey could play a role in hyperglycemia management and show potential as a natural alternative to conventional diabetes therapy. The underlying mechanisms need to be clarified, and their potential use in human diabetes therapy needs to be investigated. Elsevier Ltd 2024 Article PeerReviewed pdf en cc_by_nc_4 http://umpir.ump.edu.my/id/eprint/41098/1/Green%20honey%20of%20Banggi%20Island%20-%20A%20preliminary%20anti-diabetic%20study%20on%20zebrafish%20model.pdf ullah, Saeed and Huyop, Fahrul and Nurul Huda, . and Roswanira, Abdul Wahab and Azzmer Azzar, Abdul Hamid and Mohd Azrul Naim, Muhammad and Hajar Fauzan, Ahmad and Amir Husni, Mohd Shariff and Mohd Hamzah, Mohd Nasir (2024) Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model. Heliyon, 10 (e26469). pp. 1-8. ISSN 2405-8440. (Published) https://doi.org/10.1016/j.heliyon.2024.e26469 10.1016/j.heliyon.2024.e26469 |
| spellingShingle | HD28 Management. Industrial Management ullah, Saeed Huyop, Fahrul Nurul Huda, . Roswanira, Abdul Wahab Azzmer Azzar, Abdul Hamid Mohd Azrul Naim, Muhammad Hajar Fauzan, Ahmad Amir Husni, Mohd Shariff Mohd Hamzah, Mohd Nasir Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model |
| title | Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model |
| title_full | Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model |
| title_fullStr | Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model |
| title_full_unstemmed | Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model |
| title_short | Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model |
| title_sort | green honey of banggi island: a preliminary anti-diabetic study on zebrafish model |
| topic | HD28 Management. Industrial Management |
| url | http://umpir.ump.edu.my/id/eprint/41098/ http://umpir.ump.edu.my/id/eprint/41098/ http://umpir.ump.edu.my/id/eprint/41098/ http://umpir.ump.edu.my/id/eprint/41098/1/Green%20honey%20of%20Banggi%20Island%20-%20A%20preliminary%20anti-diabetic%20study%20on%20zebrafish%20model.pdf |