Optimization of single-step pravastatin production by Penicillium brefeldianum ESF21P through response surface methodology
The aim of the present study was to optimize the fermentation process for enhanced production of pravastatin by a wild-type Penicillium brefeldianum ESF21P strain using statistical approaches. Initial screening of significant variables influencing pravastatin production was carried out using 27-3 fr...
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World Research Association
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/38688/ http://umpir.ump.edu.my/id/eprint/38688/1/Optimization%20of%20Single-step%20Pravastatin%20Production%20by%20Penicillium%20brefeldianum.pdf http://umpir.ump.edu.my/id/eprint/38688/2/Optimization%20of%20single-step%20pravastatin%20production%20by%20Penicillium%20brefeldianum%20ESF21P%20through%20response%20surface%20methodology_ABS.pdf |
| _version_ | 1848825572357046272 |
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| author | Seydametova, Emine Norazwina, Zainol Convey, Peter |
| author_facet | Seydametova, Emine Norazwina, Zainol Convey, Peter |
| author_sort | Seydametova, Emine |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The aim of the present study was to optimize the fermentation process for enhanced production of pravastatin by a wild-type Penicillium brefeldianum ESF21P strain using statistical approaches. Initial screening of significant variables influencing pravastatin production was carried out using 27-3 fractional factorial design. The seven variables involved in this study were slant age, spore concentration, inoculum volume, fermentation time, temperature, initial pH of the medium and agitation rate. Amongst these, slant age, fermentation time, initial pH of the medium and agitation rate had significant influences on pravastatin accumulation. These four variables were further optimized using the central composite rotatable design of response surface methodology. The analysis revealed that the optimal values of the selected variables were a slant age of 5.95 days, fermentation time of 11.87 days, initial pH of the medium of 6.13 and agitation rate of 211.31 rpm (rounded to 210 rpm). These optimized conditions resulted in a maximum level of pravastatin accumulation (234.36 mg/L), approaching the value predicted by the model of 251.19 mg/L. This study confirmed that statistical approaches can be successfully applied as practical tools in improving single-step production of pravastatin by Penicillium brefeldianum ESF21P. |
| first_indexed | 2025-11-15T03:31:03Z |
| format | Article |
| id | ump-38688 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T03:31:03Z |
| publishDate | 2023 |
| publisher | World Research Association |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-386882023-10-23T03:19:42Z http://umpir.ump.edu.my/id/eprint/38688/ Optimization of single-step pravastatin production by Penicillium brefeldianum ESF21P through response surface methodology Seydametova, Emine Norazwina, Zainol Convey, Peter QD Chemistry TA Engineering (General). Civil engineering (General) TP Chemical technology The aim of the present study was to optimize the fermentation process for enhanced production of pravastatin by a wild-type Penicillium brefeldianum ESF21P strain using statistical approaches. Initial screening of significant variables influencing pravastatin production was carried out using 27-3 fractional factorial design. The seven variables involved in this study were slant age, spore concentration, inoculum volume, fermentation time, temperature, initial pH of the medium and agitation rate. Amongst these, slant age, fermentation time, initial pH of the medium and agitation rate had significant influences on pravastatin accumulation. These four variables were further optimized using the central composite rotatable design of response surface methodology. The analysis revealed that the optimal values of the selected variables were a slant age of 5.95 days, fermentation time of 11.87 days, initial pH of the medium of 6.13 and agitation rate of 211.31 rpm (rounded to 210 rpm). These optimized conditions resulted in a maximum level of pravastatin accumulation (234.36 mg/L), approaching the value predicted by the model of 251.19 mg/L. This study confirmed that statistical approaches can be successfully applied as practical tools in improving single-step production of pravastatin by Penicillium brefeldianum ESF21P. World Research Association 2023-03 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/38688/1/Optimization%20of%20Single-step%20Pravastatin%20Production%20by%20Penicillium%20brefeldianum.pdf pdf en http://umpir.ump.edu.my/id/eprint/38688/2/Optimization%20of%20single-step%20pravastatin%20production%20by%20Penicillium%20brefeldianum%20ESF21P%20through%20response%20surface%20methodology_ABS.pdf Seydametova, Emine and Norazwina, Zainol and Convey, Peter (2023) Optimization of single-step pravastatin production by Penicillium brefeldianum ESF21P through response surface methodology. Research Journal of Biotechnology, 18 (3). pp. 49-56. ISSN 0973-6263. (Published) https://doi.org/10.25303/1803rjbt49056 https://doi.org/10.25303/1803rjbt49056 |
| spellingShingle | QD Chemistry TA Engineering (General). Civil engineering (General) TP Chemical technology Seydametova, Emine Norazwina, Zainol Convey, Peter Optimization of single-step pravastatin production by Penicillium brefeldianum ESF21P through response surface methodology |
| title | Optimization of single-step pravastatin production by Penicillium brefeldianum ESF21P through response surface methodology |
| title_full | Optimization of single-step pravastatin production by Penicillium brefeldianum ESF21P through response surface methodology |
| title_fullStr | Optimization of single-step pravastatin production by Penicillium brefeldianum ESF21P through response surface methodology |
| title_full_unstemmed | Optimization of single-step pravastatin production by Penicillium brefeldianum ESF21P through response surface methodology |
| title_short | Optimization of single-step pravastatin production by Penicillium brefeldianum ESF21P through response surface methodology |
| title_sort | optimization of single-step pravastatin production by penicillium brefeldianum esf21p through response surface methodology |
| topic | QD Chemistry TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/38688/ http://umpir.ump.edu.my/id/eprint/38688/ http://umpir.ump.edu.my/id/eprint/38688/ http://umpir.ump.edu.my/id/eprint/38688/1/Optimization%20of%20Single-step%20Pravastatin%20Production%20by%20Penicillium%20brefeldianum.pdf http://umpir.ump.edu.my/id/eprint/38688/2/Optimization%20of%20single-step%20pravastatin%20production%20by%20Penicillium%20brefeldianum%20ESF21P%20through%20response%20surface%20methodology_ABS.pdf |