Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan
Aims: Prebiotics and probiotics profoundly enhance water quality and shrimp development to tackle infectious disease in shrimp farming. This study evaluated the impact of prebiotics and probiotics treatments in water by assessing the physicochemical properties and bacterial communities in local shri...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Microbiology
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/38446/ http://umpir.ump.edu.my/id/eprint/38446/1/Characterization%20of%20bacterial%20communities%20in%20prebiotics%20and%20probiotics.pdf |
| _version_ | 1848825511617232896 |
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| author | Siew, Shing Wei Ira Nadira, Kosnin Nurul Fadzlina, Zulkifli Hajar Fauzan, Ahmad Normaiza, Z. Muhammad Fitri, Yusof Ahmad Mahfuz, Gazali |
| author_facet | Siew, Shing Wei Ira Nadira, Kosnin Nurul Fadzlina, Zulkifli Hajar Fauzan, Ahmad Normaiza, Z. Muhammad Fitri, Yusof Ahmad Mahfuz, Gazali |
| author_sort | Siew, Shing Wei |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Aims: Prebiotics and probiotics profoundly enhance water quality and shrimp development to tackle infectious disease in shrimp farming. This study evaluated the impact of prebiotics and probiotics treatments in water by assessing the physicochemical properties and bacterial communities in local shrimp ponds. Methodology and results: Water was collected from shrimp pond 1 (SP1), treated with prebiotics and probiotics, and shrimp pond 2 (SP2), treated with only prebiotics. The physicochemical parameters of water from two shrimp ponds were measured, including pH, dissolved oxygen (DO), ammonia concentration and temperature. The total environmental DNA (eDNA) was extracted from the water samples and sequenced using amplicon sequencing targeting the full length of the 16S rRNA gene region via the Oxford Nanopore Technology Flongle. The water quality analysis indicated that SP1 had better water quality than SP2 for shrimp aquaculture. The dominant phyla in both shrimp ponds were Proteobacteria and Bacteroidota. SP1 samples had unique microbiota at the phylum level, including Bdellovibrionota, Firmicutes A, Patescibacteria and unclassified Rhizobiales, Saprospiraceae, Vulcanococcus and HIMB114 at the genus level. The alpha- and beta-diversity showed insignificant differences in microbiota composition between SP1 and SP2 (p-value>0.05). Conclusion, significance and impact of study: Research findings demonstrated that the probiotic-treated shrimp pond (SP1) had better water quality and more diverse microbial communities than the shrimp pond that was not treated with probiotics (SP2). |
| first_indexed | 2025-11-15T03:30:05Z |
| format | Article |
| id | ump-38446 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:30:05Z |
| publishDate | 2023 |
| publisher | Malaysian Society for Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-384462023-08-21T06:32:50Z http://umpir.ump.edu.my/id/eprint/38446/ Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan Siew, Shing Wei Ira Nadira, Kosnin Nurul Fadzlina, Zulkifli Hajar Fauzan, Ahmad Normaiza, Z. Muhammad Fitri, Yusof Ahmad Mahfuz, Gazali Q Science (General) QR Microbiology Aims: Prebiotics and probiotics profoundly enhance water quality and shrimp development to tackle infectious disease in shrimp farming. This study evaluated the impact of prebiotics and probiotics treatments in water by assessing the physicochemical properties and bacterial communities in local shrimp ponds. Methodology and results: Water was collected from shrimp pond 1 (SP1), treated with prebiotics and probiotics, and shrimp pond 2 (SP2), treated with only prebiotics. The physicochemical parameters of water from two shrimp ponds were measured, including pH, dissolved oxygen (DO), ammonia concentration and temperature. The total environmental DNA (eDNA) was extracted from the water samples and sequenced using amplicon sequencing targeting the full length of the 16S rRNA gene region via the Oxford Nanopore Technology Flongle. The water quality analysis indicated that SP1 had better water quality than SP2 for shrimp aquaculture. The dominant phyla in both shrimp ponds were Proteobacteria and Bacteroidota. SP1 samples had unique microbiota at the phylum level, including Bdellovibrionota, Firmicutes A, Patescibacteria and unclassified Rhizobiales, Saprospiraceae, Vulcanococcus and HIMB114 at the genus level. The alpha- and beta-diversity showed insignificant differences in microbiota composition between SP1 and SP2 (p-value>0.05). Conclusion, significance and impact of study: Research findings demonstrated that the probiotic-treated shrimp pond (SP1) had better water quality and more diverse microbial communities than the shrimp pond that was not treated with probiotics (SP2). Malaysian Society for Microbiology 2023 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/38446/1/Characterization%20of%20bacterial%20communities%20in%20prebiotics%20and%20probiotics.pdf Siew, Shing Wei and Ira Nadira, Kosnin and Nurul Fadzlina, Zulkifli and Hajar Fauzan, Ahmad and Normaiza, Z. and Muhammad Fitri, Yusof and Ahmad Mahfuz, Gazali (2023) Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan. Malaysian Journal Of Microbiology, 19 (4). pp. 435-446. ISSN 2231-7538. (Published) http://mjm.usm.my/uploads/issues/1845/Formatted%20MJM2-22-0048-ready%20colour.pdf |
| spellingShingle | Q Science (General) QR Microbiology Siew, Shing Wei Ira Nadira, Kosnin Nurul Fadzlina, Zulkifli Hajar Fauzan, Ahmad Normaiza, Z. Muhammad Fitri, Yusof Ahmad Mahfuz, Gazali Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan |
| title | Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan |
| title_full | Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan |
| title_fullStr | Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan |
| title_full_unstemmed | Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan |
| title_short | Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan |
| title_sort | characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from kuantan |
| topic | Q Science (General) QR Microbiology |
| url | http://umpir.ump.edu.my/id/eprint/38446/ http://umpir.ump.edu.my/id/eprint/38446/ http://umpir.ump.edu.my/id/eprint/38446/1/Characterization%20of%20bacterial%20communities%20in%20prebiotics%20and%20probiotics.pdf |