Applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: A short review
Redox enzyme and protein modified biosensors are commercially triumphant bioelectronic devices used in the point-of-care analysis. The use of nanotechnology derived nanomaterials during enzyme immobilization creates a synergistic effect by integrating enzyme’s recognition and catalytic properties wi...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Malaysia Pahang
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/38328/ http://umpir.ump.edu.my/id/eprint/38328/1/Applications%20of%20Nanomaterials%20for%20Biosensor%20Fabrication.pdf |
| _version_ | 1848825480912830464 |
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| author | Kader, Md. Ashraful Kafi, A. K. M. Nina Suhaity, Azmi |
| author_facet | Kader, Md. Ashraful Kafi, A. K. M. Nina Suhaity, Azmi |
| author_sort | Kader, Md. Ashraful |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Redox enzyme and protein modified biosensors are commercially triumphant bioelectronic devices used in the point-of-care analysis. The use of nanotechnology derived nanomaterials during enzyme immobilization creates a synergistic effect by integrating enzyme’s recognition and catalytic properties with the electronic properties of nanomaterials. This synergy improves the biosensor’s sensitivity, conductivity stability, surface-to-volume ratio, selectivity, detection limit and other analytical features. This critical review focuses on the redox enzymes and proteins most frequently used in glucose and hydrogen peroxide sensing, such as horseradish peroxidase (HRP), glucose oxidase (GOx), hemoglobin (HB), and cytochrome C (Cyt c). Besides, we evaluate the state of art of this approach, selection of nanomaterials, preparation and immobilization mechanisms, their role and sensing applications. Besides advantages, the discussions have discussed on the pressing challenges of developing these sensors. This review will guide the research community to develop rational and highly efficient nanomaterial immobilized biosensors. |
| first_indexed | 2025-11-15T03:29:36Z |
| format | Article |
| id | ump-38328 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:29:36Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Malaysia Pahang |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-383282023-08-10T07:11:16Z http://umpir.ump.edu.my/id/eprint/38328/ Applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: A short review Kader, Md. Ashraful Kafi, A. K. M. Nina Suhaity, Azmi QD Chemistry TP Chemical technology Redox enzyme and protein modified biosensors are commercially triumphant bioelectronic devices used in the point-of-care analysis. The use of nanotechnology derived nanomaterials during enzyme immobilization creates a synergistic effect by integrating enzyme’s recognition and catalytic properties with the electronic properties of nanomaterials. This synergy improves the biosensor’s sensitivity, conductivity stability, surface-to-volume ratio, selectivity, detection limit and other analytical features. This critical review focuses on the redox enzymes and proteins most frequently used in glucose and hydrogen peroxide sensing, such as horseradish peroxidase (HRP), glucose oxidase (GOx), hemoglobin (HB), and cytochrome C (Cyt c). Besides, we evaluate the state of art of this approach, selection of nanomaterials, preparation and immobilization mechanisms, their role and sensing applications. Besides advantages, the discussions have discussed on the pressing challenges of developing these sensors. This review will guide the research community to develop rational and highly efficient nanomaterial immobilized biosensors. Penerbit Universiti Malaysia Pahang 2023-06 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/38328/1/Applications%20of%20Nanomaterials%20for%20Biosensor%20Fabrication.pdf Kader, Md. Ashraful and Kafi, A. K. M. and Nina Suhaity, Azmi (2023) Applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: A short review. Current Science and Technology (CST), 2 (2). pp. 20-28. ISSN 2785-8804. (Published) https://doi.org/10.15282/cst.v2i2.9291 https://doi.org/10.15282/cst.v2i2.9291 |
| spellingShingle | QD Chemistry TP Chemical technology Kader, Md. Ashraful Kafi, A. K. M. Nina Suhaity, Azmi Applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: A short review |
| title | Applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: A short review |
| title_full | Applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: A short review |
| title_fullStr | Applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: A short review |
| title_full_unstemmed | Applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: A short review |
| title_short | Applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: A short review |
| title_sort | applications of nanomaterials for biosensor fabrication based on redox enzyme and protein: a short review |
| topic | QD Chemistry TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/38328/ http://umpir.ump.edu.my/id/eprint/38328/ http://umpir.ump.edu.my/id/eprint/38328/ http://umpir.ump.edu.my/id/eprint/38328/1/Applications%20of%20Nanomaterials%20for%20Biosensor%20Fabrication.pdf |