A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest
This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Feira de Santana
2022
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/38075/ http://umpir.ump.edu.my/id/eprint/38075/1/A%20Novel%20Integrative%20Methodology%20for%20Research%20on%20Pot-honey%20Variations%20During%20Post-harvest.pdf |
| _version_ | 1848825416108736512 |
|---|---|
| author | Patricia, Vit Bajaree, Chuttong Norhasnida, Zawawi Maria, Diaz Jane Van, der Meulen Hajar F., Ahmad Francisco A., Tomas-Barberan Gina, Meccia Khanchai, Danmek J. Enrique, Moreno David W., Roubik Ortrud Monika, Barth Dirk W., Lachenmeier Michael S., Engel |
| author_facet | Patricia, Vit Bajaree, Chuttong Norhasnida, Zawawi Maria, Diaz Jane Van, der Meulen Hajar F., Ahmad Francisco A., Tomas-Barberan Gina, Meccia Khanchai, Danmek J. Enrique, Moreno David W., Roubik Ortrud Monika, Barth Dirk W., Lachenmeier Michael S., Engel |
| author_sort | Patricia, Vit |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations. |
| first_indexed | 2025-11-15T03:28:34Z |
| format | Article |
| id | ump-38075 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:28:34Z |
| publishDate | 2022 |
| publisher | Universidade Estadual de Feira de Santana |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-380752023-07-26T05:12:44Z http://umpir.ump.edu.my/id/eprint/38075/ A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest Patricia, Vit Bajaree, Chuttong Norhasnida, Zawawi Maria, Diaz Jane Van, der Meulen Hajar F., Ahmad Francisco A., Tomas-Barberan Gina, Meccia Khanchai, Danmek J. Enrique, Moreno David W., Roubik Ortrud Monika, Barth Dirk W., Lachenmeier Michael S., Engel HD Industries. Land use. Labor Q Science (General) This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations. Universidade Estadual de Feira de Santana 2022-11 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/38075/1/A%20Novel%20Integrative%20Methodology%20for%20Research%20on%20Pot-honey%20Variations%20During%20Post-harvest.pdf Patricia, Vit and Bajaree, Chuttong and Norhasnida, Zawawi and Maria, Diaz and Jane Van, der Meulen and Hajar F., Ahmad and Francisco A., Tomas-Barberan and Gina, Meccia and Khanchai, Danmek and J. Enrique, Moreno and David W., Roubik and Ortrud Monika, Barth and Dirk W., Lachenmeier and Michael S., Engel (2022) A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest. Sociobiology, 69 (4). e8251. ISSN 0361-6525. (Published) https://doi.org/10.13102%2Fsociobiology.v69i4.8251 10.13102%2Fsociobiology.v69i4.8251 |
| spellingShingle | HD Industries. Land use. Labor Q Science (General) Patricia, Vit Bajaree, Chuttong Norhasnida, Zawawi Maria, Diaz Jane Van, der Meulen Hajar F., Ahmad Francisco A., Tomas-Barberan Gina, Meccia Khanchai, Danmek J. Enrique, Moreno David W., Roubik Ortrud Monika, Barth Dirk W., Lachenmeier Michael S., Engel A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_full | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_fullStr | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_full_unstemmed | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_short | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_sort | novel integrative methodology for research on pot-honey variations during post-harvest |
| topic | HD Industries. Land use. Labor Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/38075/ http://umpir.ump.edu.my/id/eprint/38075/ http://umpir.ump.edu.my/id/eprint/38075/ http://umpir.ump.edu.my/id/eprint/38075/1/A%20Novel%20Integrative%20Methodology%20for%20Research%20on%20Pot-honey%20Variations%20During%20Post-harvest.pdf |