Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation
Lung cancer is currently the most prevalent cause of cancer mortality due to late diagnosis and lack of curative therapies. Docetaxel (Dtx) is clinically proven as effective, but poor aqueous solubility and non-selective cytotoxicity limit its therapeutic efficacy. In this work, a nanostructured lip...
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| Format: | Article |
| Language: | English |
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MDPI
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/38052/ http://umpir.ump.edu.my/id/eprint/38052/1/pharmaceutics-15-01319.pdf |
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| author | Auni Hamimi, Idris Che Azurahanim, Che Abdullah Nor Azah, Yusof Azren Aida, Asmawi Mohd Basyaruddin, Abdul Rahman |
| author_facet | Auni Hamimi, Idris Che Azurahanim, Che Abdullah Nor Azah, Yusof Azren Aida, Asmawi Mohd Basyaruddin, Abdul Rahman |
| author_sort | Auni Hamimi, Idris |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Lung cancer is currently the most prevalent cause of cancer mortality due to late diagnosis and lack of curative therapies. Docetaxel (Dtx) is clinically proven as effective, but poor aqueous solubility and non-selective cytotoxicity limit its therapeutic efficacy. In this work, a nanostructured lipid carrier (NLC) loaded with iron oxide nanoparticles (IONP) and Dtx (Dtx-MNLC) was developed as a potential theranostic agent for lung cancer treatment. The amount of IONP and Dtx loaded into the Dtx-MNLC was quantified using Inductively Coupled Plasma Optical Emission Spectroscopy and high-performance liquid chromatography. Dtx-MNLC was then subjected to an assessment of physicochemical characteristics, in vitro drug release, and cytotoxicity. Dtx loading percentage was determined at 3.98 w/w, and 0.36 mg/mL IONP was loaded into the Dtx-MNLC. The formulation showed a biphasic drug release in a simulated cancer cell microenvironment, where 40 of Dtx was released for the first 6 h, and 80 cumulative release was achieved after 48 h. Dtx-MNLC exhibited higher cytotoxicity to A549 cells than MRC5 in a dose-dependent manner. Furthermore, the toxicity of Dtx-MNLC to MRC5 was lower than the commercial formulation. In conclusion, Dtx-MNLC shows the efficacy to inhibit lung cancer cell growth, yet it reduced toxicity on healthy lung cells and is potentially capable as a theranostic agent for lung cancer treatment. |
| first_indexed | 2025-11-15T03:28:29Z |
| format | Article |
| id | ump-38052 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:28:29Z |
| publishDate | 2023 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-380522023-07-27T08:22:17Z http://umpir.ump.edu.my/id/eprint/38052/ Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation Auni Hamimi, Idris Che Azurahanim, Che Abdullah Nor Azah, Yusof Azren Aida, Asmawi Mohd Basyaruddin, Abdul Rahman Q Science (General) QD Chemistry T Technology (General) TP Chemical technology Lung cancer is currently the most prevalent cause of cancer mortality due to late diagnosis and lack of curative therapies. Docetaxel (Dtx) is clinically proven as effective, but poor aqueous solubility and non-selective cytotoxicity limit its therapeutic efficacy. In this work, a nanostructured lipid carrier (NLC) loaded with iron oxide nanoparticles (IONP) and Dtx (Dtx-MNLC) was developed as a potential theranostic agent for lung cancer treatment. The amount of IONP and Dtx loaded into the Dtx-MNLC was quantified using Inductively Coupled Plasma Optical Emission Spectroscopy and high-performance liquid chromatography. Dtx-MNLC was then subjected to an assessment of physicochemical characteristics, in vitro drug release, and cytotoxicity. Dtx loading percentage was determined at 3.98 w/w, and 0.36 mg/mL IONP was loaded into the Dtx-MNLC. The formulation showed a biphasic drug release in a simulated cancer cell microenvironment, where 40 of Dtx was released for the first 6 h, and 80 cumulative release was achieved after 48 h. Dtx-MNLC exhibited higher cytotoxicity to A549 cells than MRC5 in a dose-dependent manner. Furthermore, the toxicity of Dtx-MNLC to MRC5 was lower than the commercial formulation. In conclusion, Dtx-MNLC shows the efficacy to inhibit lung cancer cell growth, yet it reduced toxicity on healthy lung cells and is potentially capable as a theranostic agent for lung cancer treatment. MDPI 2023 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/38052/1/pharmaceutics-15-01319.pdf Auni Hamimi, Idris and Che Azurahanim, Che Abdullah and Nor Azah, Yusof and Azren Aida, Asmawi and Mohd Basyaruddin, Abdul Rahman (2023) Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation. Pharmaceutics, 15 (5). pp. 1-19. ISSN 1999-4923. (Published) https://doi.org/10.3390/pharmaceutics15051319 https://doi.org/10.3390/pharmaceutics15051319 |
| spellingShingle | Q Science (General) QD Chemistry T Technology (General) TP Chemical technology Auni Hamimi, Idris Che Azurahanim, Che Abdullah Nor Azah, Yusof Azren Aida, Asmawi Mohd Basyaruddin, Abdul Rahman Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation |
| title | Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation |
| title_full | Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation |
| title_fullStr | Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation |
| title_full_unstemmed | Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation |
| title_short | Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation |
| title_sort | nanostructured lipid carrier co-loaded with docetaxel and magnetic nanoparticles: physicochemical characterization and in vitro evaluation |
| topic | Q Science (General) QD Chemistry T Technology (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/38052/ http://umpir.ump.edu.my/id/eprint/38052/ http://umpir.ump.edu.my/id/eprint/38052/ http://umpir.ump.edu.my/id/eprint/38052/1/pharmaceutics-15-01319.pdf |