Complete whole genome sequencing of Vibrio parahaemolyticus strain UMP001VA, isolated from sea cucumber Holothuria leucospilota from Malaysia harbouring blaCARB-33 gene
Pathogenic strain of Vibrio parahaemolyticus found in marine and coastal ecosystems can cause infections in marine animals. Increasing antibiotic resistance reported in Vibrio species may be linked to environmental contamination. Here, we present a 4.99-Mbp complete genome sequence of V. parahaemoly...
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| Language: | English English |
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Elsevier Ltd
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/37964/ http://umpir.ump.edu.my/id/eprint/37964/1/Complete%20whole%20genome%20sequencing%20of%20Vibrio%20parahaemolyticus%20.pdf http://umpir.ump.edu.my/id/eprint/37964/2/Complete%20whole%20genome%20sequencing%20of%20Vibrio%20parahaemolyticus_FULL.pdf |
| _version_ | 1848825391627632640 |
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| author | Choo, Mei Yen Siew, Shing Wei Najatul Su-Ad, Abdullah Mohd Azrul Naim, Muhammad Hajar Fauzan, Ahmad |
| author_facet | Choo, Mei Yen Siew, Shing Wei Najatul Su-Ad, Abdullah Mohd Azrul Naim, Muhammad Hajar Fauzan, Ahmad |
| author_sort | Choo, Mei Yen |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Pathogenic strain of Vibrio parahaemolyticus found in marine and coastal ecosystems can cause infections in marine animals. Increasing antibiotic resistance reported in Vibrio species may be linked to environmental contamination. Here, we present a 4.99-Mbp complete genome sequence of V. parahaemolyticus strain UMP001VA, a Gram-negative bacterium that was isolated from the gut content of a sea cucumber Holothuria leucospilota collected from Pulau Rhu, Terengganu, Malaysia. The whole genome sequencing was performed using hybrid de novo assembly using Illumina and the Oxford Nanopore Technology platforms. The genome analysis reported a total length of 4,991,208 bp of V. parahaemolyticus genome sequences with 45.3 % GC content. The functional annotation of the genome showcased that 56 genes of V. parahaemolyticus were associated with virulence, disease, and defence function. Virulence factors found in H. leucospilota were related to T3SS1, together with the presence of blaCARB-33 antibiotic resistance gene conferring ampicillin, piperacillin, and amoxicillin drug resistance were reported. The presence of virulence factors and antibiotic resistance genes reveals the considerable pathogenic potential of V. parahaemolyticus in sea cucumbers. Therefore, the whole genome sequencing presented here provides a fundamental genome insight for future assessment of V. parahaemolyticus in marine organisms. |
| first_indexed | 2025-11-15T03:28:11Z |
| format | Article |
| id | ump-37964 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T03:28:11Z |
| publishDate | 2023 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-379642023-07-07T02:01:57Z http://umpir.ump.edu.my/id/eprint/37964/ Complete whole genome sequencing of Vibrio parahaemolyticus strain UMP001VA, isolated from sea cucumber Holothuria leucospilota from Malaysia harbouring blaCARB-33 gene Choo, Mei Yen Siew, Shing Wei Najatul Su-Ad, Abdullah Mohd Azrul Naim, Muhammad Hajar Fauzan, Ahmad Q Science (General) TP Chemical technology Pathogenic strain of Vibrio parahaemolyticus found in marine and coastal ecosystems can cause infections in marine animals. Increasing antibiotic resistance reported in Vibrio species may be linked to environmental contamination. Here, we present a 4.99-Mbp complete genome sequence of V. parahaemolyticus strain UMP001VA, a Gram-negative bacterium that was isolated from the gut content of a sea cucumber Holothuria leucospilota collected from Pulau Rhu, Terengganu, Malaysia. The whole genome sequencing was performed using hybrid de novo assembly using Illumina and the Oxford Nanopore Technology platforms. The genome analysis reported a total length of 4,991,208 bp of V. parahaemolyticus genome sequences with 45.3 % GC content. The functional annotation of the genome showcased that 56 genes of V. parahaemolyticus were associated with virulence, disease, and defence function. Virulence factors found in H. leucospilota were related to T3SS1, together with the presence of blaCARB-33 antibiotic resistance gene conferring ampicillin, piperacillin, and amoxicillin drug resistance were reported. The presence of virulence factors and antibiotic resistance genes reveals the considerable pathogenic potential of V. parahaemolyticus in sea cucumbers. Therefore, the whole genome sequencing presented here provides a fundamental genome insight for future assessment of V. parahaemolyticus in marine organisms. Elsevier Ltd 2023-01 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/37964/1/Complete%20whole%20genome%20sequencing%20of%20Vibrio%20parahaemolyticus%20.pdf pdf en http://umpir.ump.edu.my/id/eprint/37964/2/Complete%20whole%20genome%20sequencing%20of%20Vibrio%20parahaemolyticus_FULL.pdf Choo, Mei Yen and Siew, Shing Wei and Najatul Su-Ad, Abdullah and Mohd Azrul Naim, Muhammad and Hajar Fauzan, Ahmad (2023) Complete whole genome sequencing of Vibrio parahaemolyticus strain UMP001VA, isolated from sea cucumber Holothuria leucospilota from Malaysia harbouring blaCARB-33 gene. Materials Today: Proceedings, 75. pp. 68-73. ISSN 2214-7853. (Published) https://doi.org/10.1016/j.matpr.2022.10.042 https://doi.org/10.1016/j.matpr.2022.10.042 |
| spellingShingle | Q Science (General) TP Chemical technology Choo, Mei Yen Siew, Shing Wei Najatul Su-Ad, Abdullah Mohd Azrul Naim, Muhammad Hajar Fauzan, Ahmad Complete whole genome sequencing of Vibrio parahaemolyticus strain UMP001VA, isolated from sea cucumber Holothuria leucospilota from Malaysia harbouring blaCARB-33 gene |
| title | Complete whole genome sequencing of Vibrio parahaemolyticus strain UMP001VA, isolated from sea cucumber Holothuria leucospilota from Malaysia harbouring blaCARB-33 gene |
| title_full | Complete whole genome sequencing of Vibrio parahaemolyticus strain UMP001VA, isolated from sea cucumber Holothuria leucospilota from Malaysia harbouring blaCARB-33 gene |
| title_fullStr | Complete whole genome sequencing of Vibrio parahaemolyticus strain UMP001VA, isolated from sea cucumber Holothuria leucospilota from Malaysia harbouring blaCARB-33 gene |
| title_full_unstemmed | Complete whole genome sequencing of Vibrio parahaemolyticus strain UMP001VA, isolated from sea cucumber Holothuria leucospilota from Malaysia harbouring blaCARB-33 gene |
| title_short | Complete whole genome sequencing of Vibrio parahaemolyticus strain UMP001VA, isolated from sea cucumber Holothuria leucospilota from Malaysia harbouring blaCARB-33 gene |
| title_sort | complete whole genome sequencing of vibrio parahaemolyticus strain ump001va, isolated from sea cucumber holothuria leucospilota from malaysia harbouring blacarb-33 gene |
| topic | Q Science (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/37964/ http://umpir.ump.edu.my/id/eprint/37964/ http://umpir.ump.edu.my/id/eprint/37964/ http://umpir.ump.edu.my/id/eprint/37964/1/Complete%20whole%20genome%20sequencing%20of%20Vibrio%20parahaemolyticus%20.pdf http://umpir.ump.edu.my/id/eprint/37964/2/Complete%20whole%20genome%20sequencing%20of%20Vibrio%20parahaemolyticus_FULL.pdf |