qPCR analysis of quorum sensing genes of pseudomonas aeruginosa: Primer design and qPCR efficiency

QR Code

qPCR analysis of quorum sensing genes of pseudomonas aeruginosa: Primer design and qPCR efficiency

A well-designed and properly validated primer produces a specific and efficient qPCR assay. The primer’s melting temperature, G-C content, its size, as well as the amplicon’s size are the principal considerations while designing the primer for this study and it was in accordance with the MIQE guidel...

Full description

Bibliographic Details
Main Authors:	Saiedatul Akmal, Mohamed Sedek, Mohd Azmir, Arifin, Mimi Sakinah, Abdul Munaim
Format:	Article
Language:	English
Published:	Penerbit Universiti Malaysia Pahang 2023
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/37835/ http://umpir.ump.edu.my/id/eprint/37835/1/qPCR%20Analysis%20of%20Quorum%20Sensing%20Genes%20of%20Pseudomonas%20aeruginosa.pdf

Similar Items

Optimization of qPCR assay of chicken cytokine genes using sybr green method
by: Chan, Jia Hui
Published: (2015)

Development of thermostable multiplex qPCR for simultaneous detection of pathogens associated with febrile illnesses
by: Ali, Mohammad Ridhuan Mohd
Published: (2018)

Isolation and selection of reference genes for Ganoderma boninense gene expression study using quantitative real-time PCR (qPCR)
by: Lim, Fook Hwa, et al.
Published: (2014)

Biological control of Leptosphaeria maculans on Brassica napus and quantification of the microbes in planta using qPCR
by: Cholerton, Linda Jane
Published: (2015)

Design and development of multiplex real-time pcr (qPCR) for detection of streptococcus pneumoniae, klebsiella pneumoniae and haemophilus influenzae
by: Zahari, Nurul Izzaty Najwa
Published: (2024)

DNA-Based Faecal Dietary Analysis: A Comparison of qPCR and High Throughput Sequencing Approaches
by: Murray, D., et al.
Published: (2011)

Identification of oil palm root-specific genes through mining of RNA-Seq data and RT-qPCR analysis
by: Subhi, Siti Masura, et al.
Published: (2021)

Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review
by: Che-Engku-Chik, Che Engku Noramalina, et al.
Published: (2016)

Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus)
by: Harper, Lynsey R., et al.
Published: (2018)

Lard traceability in lard-adulterated starch-based food using qPCR and evaluation of chemometrics and random forest on GC-MS chromatogram
by: Azizan, Nur Inani
Published: (2021)

RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4
by: Munusamy, Umaiyal, et al.
Published: (2019)

Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa
by: Saiedatul Akmal, Mohamed Sedek, et al.
Published: (2021)

Expression analysis using reverse transcription quantitative Real-Time PCR (RT-qPCR) suggests different strategies in Parageobacillus caldoxylosilyticus ER4B under exposure to cold shock
by: Ching, Xin Jie, et al.
Published: (2022)

Spatial distribution of toxic Alexandrium tamiyavanichii (Dinophyceae) in the southeastern South China Sea-Sulu Sea: A molecular-based assessment using real-time quantitative PCR (qPCR) assay
by: Nyuk, Fong Kon, et al.
Published: (2015)

Expression analysis using reverse transcription quantitative Real-Time PCR (RT-qPCR) suggests different strategies in Parageobacillus caldoxylosilyticus ER4B under exposure to cold shock
by: Jie, Ching Xin, et al.
Published: (2022)

Performance and caecal microbial community determined using qPCR and V3 region of 16S rRNA gene sequencing in laying hens fed prebiotic, probiotic and synbiotic diets
by: Tang, Shirley Gee Hoon, et al.
Published: (2014)

Sensitive detection of PIK3CA Exon 20 H1047R breast cancer based on lowcost intercalary dye SYBR Green I real-time qPCR assay
by: Desriani,, et al.
Published: (2024)

Important considerations in qRT-PCR
by: Phang, Ing Chia
Published: (2011)

Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay
by: Saiedatul Akmal, Mohamed Sedek, et al.
Published: (2020)

Interplay between quorum sensing and metabolism in Pseudomonas aeruginosa
by: Ruparell, Avika
Published: (2012)

New approaches to detect and inhibit quorum sensing activity in Pseudomonas aeruginosa
by: Lafayette, I. H. G.
Published: (2016)

Post-transcriptional regulation of the Pqs Quorum Sensing System in Pseudomonas aeruginosa
by: Panagiotopoulou, Dimitra
Published: (2024)

Inhibition of Pseudomonas aeruginosa quorum sensing by Curucma xanthorrhiza roxb. extract
by: Othman, Ahmad Fiqri Mustaqim, et al.
Published: (2019)

The effects of Pseudomonas aeruginosa quorum sensing signalling molecules on human T cell function
by: Huynh, Tina
Published: (2008)

Pseudomonas aeruginosa quorum sensing molecules correlate with clinical status in cystic fibrosis
by: Barr, Helen L., et al.
Published: (2015)

Pseudomonas aeruginosa: host interactions and the identification of novel modulators of quorum sensing-mediated virulence
by: Bates, Robyn Hannah
Published: (2014)

New vaccines for infectious diseases: immunological targeting of the quorum sensing system of Pseudomonas aeruginosa
by: Pathak, Aditi
Published: (2012)

Lectin-mediated biofilm maturation, quorum sensing and Pseudomonas aeruginosa infections in cystic fibrosis
by: Crusz, S.A.
Published: (2009)

In silico and in vitro-guided identification of inhibitors of alkylquinolone-dependent quorum sensing in Pseudomonas aeruginosa
by: Soukarieh, Fadi, et al.
Published: (2018)

Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
by: A. S., Fadzlina, et al.
Published: (2013)

Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
by: Yee, May Ye
Published: (2020)

Differential regulation of the phenazine biosynthetic operons by quorum sensing in Pseudomonas aeruginosa PAO1-N
by: Higgins, Steven, et al.
Published: (2018)

Pseudomonas aeruginosa quorum sensing systems as drug discovery targets: current position and future perspectives
by: Soukarieh, Fadi, et al.
Published: (2018)

Structural investigation of Pseudomonas aeruginosa quorum sensing receptor PqsR to aid virulence inhibition
by: Richardson, William
Published: (2022)

Novel approaches to tackling antimicrobial resistance: the design, synthesis and evaluation of quorum sensing inhibitors in Pseudomonas aeruginosa
by: Grossman, Scott
Published: (2022)

Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)
by: Abllah, Zurainie, et al.
Published: (2019)

New combination of primer pairs for PCR-DGGE detection of soil ciliates
by: Lee, Ting Ting, et al.
Published: (2015)

Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR
by: Abdul Talib, Mohd Afendy, et al.
Published: (2015)

Selection and validation of reference genes for qRT-PCR expression analyses of leaf, flower and capsules of Impatiens balsamina (Balsaminaceae)
by: Foong, Lian Chee, et al.
Published: (2019)

Unravelling the genome-wide contributions of specific 2-alkyl-4-quinolones and PqsE to quorum sensing in Pseudomonas aeruginosa
by: Rampioni, Giordano, et al.
Published: (2016)