Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier
Vero cells are continuous mammalian cell lines that are commonly used in research, viral vaccines production, recombinant protein production, and many others. The main objective of this study was to adapt the Vero cells in serum-free medium (SFM) from serum supplemented medium (SSM) prior to culture...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2022
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| Online Access: | http://umpir.ump.edu.my/id/eprint/36881/ http://umpir.ump.edu.my/id/eprint/36881/1/Sequential%20adaptation%20to%20serum-free%20medium%20for%20vero%20cells%20cultivation%20on%20ultraviolet_ozone%20%28uvo%29%20treated%20microcarrier.pdf |
| _version_ | 1848825109330001920 |
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| author | Swan, Sia Yiik Nurul Izzati, Azahar Lansayan, Jalviana Wong, Judy Mohd Azmir, Arifin |
| author_facet | Swan, Sia Yiik Nurul Izzati, Azahar Lansayan, Jalviana Wong, Judy Mohd Azmir, Arifin |
| author_sort | Swan, Sia Yiik |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Vero cells are continuous mammalian cell lines that are commonly used in research, viral vaccines production, recombinant protein production, and many others. The main objective of this study was to adapt the Vero cells in serum-free medium (SFM) from serum supplemented medium (SSM) prior to culture upscale from T-flask to spinner vessel using ultraviolet/ozone (UVO) treated polystyrene (PS) and polycaprolactone (PCL) microcarriers. The number of cells was counted using hemocytometer and microscope. From the results, it was observed that the maximum cell concentration of SFM adapted cells is higher than the cells cultured in SSM along with shorter doubling time. Culture of Vero cells in spinner vessel showed lower maximum cell concentrations compared to in T-flask due to several factors such as cell inoculum size, hydrophilicity of microcarriers and elevated temperature during incubation. |
| first_indexed | 2025-11-15T03:23:42Z |
| format | Conference or Workshop Item |
| id | ump-36881 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:23:42Z |
| publishDate | 2022 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-368812024-01-10T08:05:35Z http://umpir.ump.edu.my/id/eprint/36881/ Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier Swan, Sia Yiik Nurul Izzati, Azahar Lansayan, Jalviana Wong, Judy Mohd Azmir, Arifin QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Vero cells are continuous mammalian cell lines that are commonly used in research, viral vaccines production, recombinant protein production, and many others. The main objective of this study was to adapt the Vero cells in serum-free medium (SFM) from serum supplemented medium (SSM) prior to culture upscale from T-flask to spinner vessel using ultraviolet/ozone (UVO) treated polystyrene (PS) and polycaprolactone (PCL) microcarriers. The number of cells was counted using hemocytometer and microscope. From the results, it was observed that the maximum cell concentration of SFM adapted cells is higher than the cells cultured in SSM along with shorter doubling time. Culture of Vero cells in spinner vessel showed lower maximum cell concentrations compared to in T-flask due to several factors such as cell inoculum size, hydrophilicity of microcarriers and elevated temperature during incubation. 2022-11-15 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/36881/1/Sequential%20adaptation%20to%20serum-free%20medium%20for%20vero%20cells%20cultivation%20on%20ultraviolet_ozone%20%28uvo%29%20treated%20microcarrier.pdf Swan, Sia Yiik and Nurul Izzati, Azahar and Lansayan, Jalviana and Wong, Judy and Mohd Azmir, Arifin (2022) Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier. In: The 6th National Conference for Postgraduate Research (NCON-PGR 2022) , 15 November 2022 , Virtual Conference, Universiti Malaysia Pahang, Malaysia. p. 94.. (Published) https://ncon-pgr.ump.edu.my/index.php/en/?option=com_fileman&view=file&routed=1&name=E-BOOK%20NCON%202022%20.pdf&folder=E-BOOK%20NCON%202022&container=fileman-files |
| spellingShingle | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Swan, Sia Yiik Nurul Izzati, Azahar Lansayan, Jalviana Wong, Judy Mohd Azmir, Arifin Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier |
| title | Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier |
| title_full | Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier |
| title_fullStr | Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier |
| title_full_unstemmed | Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier |
| title_short | Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier |
| title_sort | sequential adaptation to serum-free medium for vero cells cultivation on ultraviolet/ozone (uvo) treated microcarrier |
| topic | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/36881/ http://umpir.ump.edu.my/id/eprint/36881/ http://umpir.ump.edu.my/id/eprint/36881/1/Sequential%20adaptation%20to%20serum-free%20medium%20for%20vero%20cells%20cultivation%20on%20ultraviolet_ozone%20%28uvo%29%20treated%20microcarrier.pdf |