Optimization of active protein extraction from biomass waste for application in water treatment
In this study, the effect of the extraction medium on the properties and efficiency of bio-coagulant, extracted of Jatropha curcas (Jc), in turbidity removal from aqueous solutions has been investigated. The optimised values of NaCI concentration (i.e. NaCI), solution pH and solution temperature wer...
| Main Authors: | , , , |
|---|---|
| Format: | Research Report |
| Language: | English |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/36429/ http://umpir.ump.edu.my/id/eprint/36429/1/Optimization%20of%20active%20protein%20extraction%20from%20biomass%20waste%20for%20application%20in%20water%20treatment.wm.pdf |
| _version_ | 1848824993017757696 |
|---|---|
| author | Nasrullah, Mohd Ameedeen, Mohamed Ariff Nurul Huda, Othman Faizal, Che Ku M. |
| author_facet | Nasrullah, Mohd Ameedeen, Mohamed Ariff Nurul Huda, Othman Faizal, Che Ku M. |
| author_sort | Nasrullah, Mohd |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | In this study, the effect of the extraction medium on the properties and efficiency of bio-coagulant, extracted of Jatropha curcas (Jc), in turbidity removal from aqueous solutions has been investigated. The optimised values of NaCI concentration (i.e. NaCI), solution pH and solution temperature were identified to improve the extraction of the coagulant. The optimised conditions were associated with an optimum coagulant dosage and a maximum turbidity removal from the synthetic aqueous solutions. The highest turbidity reduction was achieved with the coagulant extracted at a solution pH of I 0 and an extraction temperature of 60 oc (pH I 0/60°C-JcPc). Under these conditions, the coagulant dosage required was reduced by 80-90%, depending on the coagulation pH. At the coagulation pH=6, the pH I 0/60°C-JcPc well reduced the turbidity by 85%. However, the distilled water-based extract failed to lower the turbidity. Several analytical techniques were employed to characterize the nature of the active components derived from Jc. SDS-PAGE electrophoresis showed that Jc extract was mainly made up of proteins with molecular weights between 20 and 35 kDa. The optimized extraction conditions significantly improved the efficiency of this promising bio-derived coagulant in turbidity reduction. This study demonstrates the potential employability of these enhanced bio-coagulants. This can be a step ahead in helping with the development of sustainable processes in (waste)water treatment, particularly in tropical regions e.g. Malaysia with an abundant access to Jc. |
| first_indexed | 2025-11-15T03:21:51Z |
| format | Research Report |
| id | ump-36429 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:21:51Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-364292023-03-02T01:24:36Z http://umpir.ump.edu.my/id/eprint/36429/ Optimization of active protein extraction from biomass waste for application in water treatment Nasrullah, Mohd Ameedeen, Mohamed Ariff Nurul Huda, Othman Faizal, Che Ku M. TD Environmental technology. Sanitary engineering In this study, the effect of the extraction medium on the properties and efficiency of bio-coagulant, extracted of Jatropha curcas (Jc), in turbidity removal from aqueous solutions has been investigated. The optimised values of NaCI concentration (i.e. NaCI), solution pH and solution temperature were identified to improve the extraction of the coagulant. The optimised conditions were associated with an optimum coagulant dosage and a maximum turbidity removal from the synthetic aqueous solutions. The highest turbidity reduction was achieved with the coagulant extracted at a solution pH of I 0 and an extraction temperature of 60 oc (pH I 0/60°C-JcPc). Under these conditions, the coagulant dosage required was reduced by 80-90%, depending on the coagulation pH. At the coagulation pH=6, the pH I 0/60°C-JcPc well reduced the turbidity by 85%. However, the distilled water-based extract failed to lower the turbidity. Several analytical techniques were employed to characterize the nature of the active components derived from Jc. SDS-PAGE electrophoresis showed that Jc extract was mainly made up of proteins with molecular weights between 20 and 35 kDa. The optimized extraction conditions significantly improved the efficiency of this promising bio-derived coagulant in turbidity reduction. This study demonstrates the potential employability of these enhanced bio-coagulants. This can be a step ahead in helping with the development of sustainable processes in (waste)water treatment, particularly in tropical regions e.g. Malaysia with an abundant access to Jc. 2017 Research Report NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/36429/1/Optimization%20of%20active%20protein%20extraction%20from%20biomass%20waste%20for%20application%20in%20water%20treatment.wm.pdf Nasrullah, Mohd and Ameedeen, Mohamed Ariff and Nurul Huda, Othman and Faizal, Che Ku M. (2017) Optimization of active protein extraction from biomass waste for application in water treatment. , [Research Report] (Unpublished) |
| spellingShingle | TD Environmental technology. Sanitary engineering Nasrullah, Mohd Ameedeen, Mohamed Ariff Nurul Huda, Othman Faizal, Che Ku M. Optimization of active protein extraction from biomass waste for application in water treatment |
| title | Optimization of active protein extraction from biomass waste for application in water treatment |
| title_full | Optimization of active protein extraction from biomass waste for application in water treatment |
| title_fullStr | Optimization of active protein extraction from biomass waste for application in water treatment |
| title_full_unstemmed | Optimization of active protein extraction from biomass waste for application in water treatment |
| title_short | Optimization of active protein extraction from biomass waste for application in water treatment |
| title_sort | optimization of active protein extraction from biomass waste for application in water treatment |
| topic | TD Environmental technology. Sanitary engineering |
| url | http://umpir.ump.edu.my/id/eprint/36429/ http://umpir.ump.edu.my/id/eprint/36429/1/Optimization%20of%20active%20protein%20extraction%20from%20biomass%20waste%20for%20application%20in%20water%20treatment.wm.pdf |