Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products
Clinacanthus nutans has attracted Malaysian public interest due to its high medicinal values in the prevention of cancer. The extraction was done by MeOH at room temperature while chromatography was done on a silica gel RP-18. Six fractions collected from column chromatography were evaluated by the...
| Main Authors: | , |
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| Format: | Research Book Profile |
| Language: | English |
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2016
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| Online Access: | http://umpir.ump.edu.my/id/eprint/36247/ http://umpir.ump.edu.my/id/eprint/36247/1/Development%20of%20chemical%20markers%20and%20chromatographic%20fingerprint%20of%20Clinachantus%20nutans%20for%20evaluation%20of%20therapeutic%20products.pdf |
| _version_ | 1848824942584397824 |
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| author | Hazrulrizawati, Abd Hamid Mashitah, Mohd Yusoff |
| author_facet | Hazrulrizawati, Abd Hamid Mashitah, Mohd Yusoff |
| author_sort | Hazrulrizawati, Abd Hamid |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Clinacanthus nutans has attracted Malaysian public interest due to its high medicinal values in the prevention of cancer. The extraction was done by MeOH at room temperature while chromatography was done on a silica gel RP-18. Six fractions collected from column chromatography were evaluated by the MTT assay against two breast cancer cell lines: MDA-MB-231 and MCF-7. Amongst the fractions, A12 and A17 were shown to exhibit the most activity, and the compounds were mostly isolated from these fractions. Two sulphur-containing compounds, entadamide C and clinamide D were isolated. Molecular docking simulation studies revealed that entadamide C and clinamide D could bind favourably to the caspase-3 binding site with the binding energy of -4.28 kcal/mol and -4.84 kcal/mol, respectively. |
| first_indexed | 2025-11-15T03:21:03Z |
| format | Research Book Profile |
| id | ump-36247 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:21:03Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-362472023-01-04T02:04:59Z http://umpir.ump.edu.my/id/eprint/36247/ Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products Hazrulrizawati, Abd Hamid Mashitah, Mohd Yusoff Q Science (General) T Technology (General) Clinacanthus nutans has attracted Malaysian public interest due to its high medicinal values in the prevention of cancer. The extraction was done by MeOH at room temperature while chromatography was done on a silica gel RP-18. Six fractions collected from column chromatography were evaluated by the MTT assay against two breast cancer cell lines: MDA-MB-231 and MCF-7. Amongst the fractions, A12 and A17 were shown to exhibit the most activity, and the compounds were mostly isolated from these fractions. Two sulphur-containing compounds, entadamide C and clinamide D were isolated. Molecular docking simulation studies revealed that entadamide C and clinamide D could bind favourably to the caspase-3 binding site with the binding energy of -4.28 kcal/mol and -4.84 kcal/mol, respectively. 2016 Research Book Profile NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/36247/1/Development%20of%20chemical%20markers%20and%20chromatographic%20fingerprint%20of%20Clinachantus%20nutans%20for%20evaluation%20of%20therapeutic%20products.pdf Hazrulrizawati, Abd Hamid and Mashitah, Mohd Yusoff (2016) Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products. , [Research Book Profile: Research Report] (Unpublished) |
| spellingShingle | Q Science (General) T Technology (General) Hazrulrizawati, Abd Hamid Mashitah, Mohd Yusoff Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products |
| title | Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products |
| title_full | Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products |
| title_fullStr | Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products |
| title_full_unstemmed | Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products |
| title_short | Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products |
| title_sort | development of chemical markers and chromatographic fingerprint of clinachantus nutans for evaluation of therapeutic products |
| topic | Q Science (General) T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/36247/ http://umpir.ump.edu.my/id/eprint/36247/1/Development%20of%20chemical%20markers%20and%20chromatographic%20fingerprint%20of%20Clinachantus%20nutans%20for%20evaluation%20of%20therapeutic%20products.pdf |