Determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition

Fenugreek an ancient plant also known as Trigonella-foenum-graecum L. This plant has achieved a great interest due to its valuable medicinal properties. The current research is targeted to extract phenolic and saponin bioactive compounds from seeds of fenugreek via microwave-assisted extraction (MAE...

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Main Authors: S., Akbari, A. H., Nour, R. M., Yunus
Format: Conference or Workshop Item
Language:English
Published: Institute of Physics Publishing 2020
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/36010/
http://umpir.ump.edu.my/id/eprint/36010/1/Determination%20of%20phenolics%20and%20saponins%20in%20fenugreek%20seed%20extracted%20via%20microwave-assisted%20extraction%20method%20at%20the%20optimal%20condition.pdf
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author S., Akbari
A. H., Nour
R. M., Yunus
author_facet S., Akbari
A. H., Nour
R. M., Yunus
author_sort S., Akbari
building UMP Institutional Repository
collection Online Access
description Fenugreek an ancient plant also known as Trigonella-foenum-graecum L. This plant has achieved a great interest due to its valuable medicinal properties. The current research is targeted to extract phenolic and saponin bioactive compounds from seeds of fenugreek via microwave-assisted extraction (MAE) technique. The seed extraction was carried out at irradiation time of (2-4 min), microwave oven power (500-700 W), ethanol concentration (4080 %), ratio of seed-to-extraction solvent (1:8-1:12 g/mL) and a constant extraction temperature (70°C). The statistical process of response surface methodology (RSM) under software of Design-Expert was used to optimize the recovery of total saponin content (TSC) and total phenolic content (TPC) in extract of fenugreek seed. One-factor-at-one-time (OFAT) was used to evaluate the levels of independent factors, and the suitable levels (-1, 0, and +1) were selected for the optimization process. Results indicated that the optimal conditions of extraction process to achieve the highest recoveries were 2.84 min, 572.50 W, 63.68% and 1:9 g/mL, respectively. Analysis of variance (ANOVA) indicated that TSC and TPC were significantly influenced by the time of irradiation, microwave oven power, solvent concentration, and ratio of seed-to-extraction solvent. FTIR results confirmed the presence of specific peaks belonging to saponins and phenolic compounds.
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format Conference or Workshop Item
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institution Universiti Malaysia Pahang
institution_category Local University
language English
last_indexed 2025-11-15T03:20:49Z
publishDate 2020
publisher Institute of Physics Publishing
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spelling ump-360102022-12-28T01:36:22Z http://umpir.ump.edu.my/id/eprint/36010/ Determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition S., Akbari A. H., Nour R. M., Yunus QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Fenugreek an ancient plant also known as Trigonella-foenum-graecum L. This plant has achieved a great interest due to its valuable medicinal properties. The current research is targeted to extract phenolic and saponin bioactive compounds from seeds of fenugreek via microwave-assisted extraction (MAE) technique. The seed extraction was carried out at irradiation time of (2-4 min), microwave oven power (500-700 W), ethanol concentration (4080 %), ratio of seed-to-extraction solvent (1:8-1:12 g/mL) and a constant extraction temperature (70°C). The statistical process of response surface methodology (RSM) under software of Design-Expert was used to optimize the recovery of total saponin content (TSC) and total phenolic content (TPC) in extract of fenugreek seed. One-factor-at-one-time (OFAT) was used to evaluate the levels of independent factors, and the suitable levels (-1, 0, and +1) were selected for the optimization process. Results indicated that the optimal conditions of extraction process to achieve the highest recoveries were 2.84 min, 572.50 W, 63.68% and 1:9 g/mL, respectively. Analysis of variance (ANOVA) indicated that TSC and TPC were significantly influenced by the time of irradiation, microwave oven power, solvent concentration, and ratio of seed-to-extraction solvent. FTIR results confirmed the presence of specific peaks belonging to saponins and phenolic compounds. Institute of Physics Publishing 2020-03-04 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/36010/1/Determination%20of%20phenolics%20and%20saponins%20in%20fenugreek%20seed%20extracted%20via%20microwave-assisted%20extraction%20method%20at%20the%20optimal%20condition.pdf S., Akbari and A. H., Nour and R. M., Yunus (2020) Determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-11., 736 (022024). ISSN 1757-8981 (Published) https://doi.org/10.1088/1757-899X/736/2/022024
spellingShingle QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
S., Akbari
A. H., Nour
R. M., Yunus
Determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition
title Determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition
title_full Determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition
title_fullStr Determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition
title_full_unstemmed Determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition
title_short Determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition
title_sort determination of phenolics and saponins in fenugreek seed extracted via microwave-assisted extraction method at the optimal condition
topic QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/36010/
http://umpir.ump.edu.my/id/eprint/36010/
http://umpir.ump.edu.my/id/eprint/36010/1/Determination%20of%20phenolics%20and%20saponins%20in%20fenugreek%20seed%20extracted%20via%20microwave-assisted%20extraction%20method%20at%20the%20optimal%20condition.pdf