Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay
Anti-quorum sensing property has been a popular alternative approach over bactericidal/bacteriostatic property in combating bacterial infection while simultaneously tackling the dilemma of antibiotic resistance. We presented pyoverdin assay as an initial screening to qualitatively determine the anti...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Institute of Physics Publishing
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/36009/ http://umpir.ump.edu.my/id/eprint/36009/1/Relative%20assessment%20of%20anti-quorum%20sensing%20in%20Piper%20betle%20leaves%20extract%20via%20pyoverdin%20assay.pdf |
| _version_ | 1848824927580323840 |
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| author | Saiedatul Akmal, Mohamed Sedek M. N., Abd Latif Mohd Azmir, Arifin Dr. Mimi Sakinah, Abdul Munaim |
| author_facet | Saiedatul Akmal, Mohamed Sedek M. N., Abd Latif Mohd Azmir, Arifin Dr. Mimi Sakinah, Abdul Munaim |
| author_sort | Saiedatul Akmal, Mohamed Sedek |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Anti-quorum sensing property has been a popular alternative approach over bactericidal/bacteriostatic property in combating bacterial infection while simultaneously tackling the dilemma of antibiotic resistance. We presented pyoverdin assay as an initial screening to qualitatively determine the anti-quorum sensing activity in Piper betle by measuring the loss of absorbance at wavelength 630 nm. Growth of Pseudomonas aeruginosa was proven to be unaffected by the presence of the P. betle leaves extract. The regression value (R2) of the quorum sensing (QS) activity in untreated supernatant of P. aeruginosa was 0.9636 and we presented the QS activity in fold-change, normalized to untreated sample for a fair comparison between batch of assays. We further assessed the QS activity in the extracts of P. betle leaves and found the QS activity of P. aeruginosa grown in the presence of ethyl acetate extract at 200 μg/ml was reduced to 0.6-fold. As the concentrations went lower, higher fold of QS activity was observed, suggesting that P. betle leaves extract is demonstrating anti-QS activity at a higher concentration. Further fractionate of ethyl acetate crude extracts resulted in three fractionates with high anti-QS activity with >50% reduction in QS activity and five fractionates with intermediate anti-QS activity. The use of pyoverdin assay to qualitatively portray the anti-QS activity could shorten the lengthiness of extracting and measuring the signaling molecule yet, produces reliable information to screen for anti-QS activity and guide for further fractionation and purification of bioactive compound. |
| first_indexed | 2025-11-15T03:20:48Z |
| format | Conference or Workshop Item |
| id | ump-36009 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:20:48Z |
| publishDate | 2020 |
| publisher | Institute of Physics Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-360092024-01-10T07:48:47Z http://umpir.ump.edu.my/id/eprint/36009/ Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay Saiedatul Akmal, Mohamed Sedek M. N., Abd Latif Mohd Azmir, Arifin Dr. Mimi Sakinah, Abdul Munaim QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Anti-quorum sensing property has been a popular alternative approach over bactericidal/bacteriostatic property in combating bacterial infection while simultaneously tackling the dilemma of antibiotic resistance. We presented pyoverdin assay as an initial screening to qualitatively determine the anti-quorum sensing activity in Piper betle by measuring the loss of absorbance at wavelength 630 nm. Growth of Pseudomonas aeruginosa was proven to be unaffected by the presence of the P. betle leaves extract. The regression value (R2) of the quorum sensing (QS) activity in untreated supernatant of P. aeruginosa was 0.9636 and we presented the QS activity in fold-change, normalized to untreated sample for a fair comparison between batch of assays. We further assessed the QS activity in the extracts of P. betle leaves and found the QS activity of P. aeruginosa grown in the presence of ethyl acetate extract at 200 μg/ml was reduced to 0.6-fold. As the concentrations went lower, higher fold of QS activity was observed, suggesting that P. betle leaves extract is demonstrating anti-QS activity at a higher concentration. Further fractionate of ethyl acetate crude extracts resulted in three fractionates with high anti-QS activity with >50% reduction in QS activity and five fractionates with intermediate anti-QS activity. The use of pyoverdin assay to qualitatively portray the anti-QS activity could shorten the lengthiness of extracting and measuring the signaling molecule yet, produces reliable information to screen for anti-QS activity and guide for further fractionation and purification of bioactive compound. Institute of Physics Publishing 2020-03-04 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/36009/1/Relative%20assessment%20of%20anti-quorum%20sensing%20in%20Piper%20betle%20leaves%20extract%20via%20pyoverdin%20assay.pdf Saiedatul Akmal, Mohamed Sedek and M. N., Abd Latif and Mohd Azmir, Arifin and Dr. Mimi Sakinah, Abdul Munaim (2020) Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay. In: IOP Conference Series: Materials Science and Engineering; Energy Security and Chemical Engineering Congress 2019, ESChE 2019 , 17-19 July 2019 , Kuala Lumpur. pp. 1-8., 736 (062025). ISSN 1757-8981 (Published) https://doi.org/10.1088/1757-899X/736/6/062025 |
| spellingShingle | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Saiedatul Akmal, Mohamed Sedek M. N., Abd Latif Mohd Azmir, Arifin Dr. Mimi Sakinah, Abdul Munaim Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay |
| title | Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay |
| title_full | Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay |
| title_fullStr | Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay |
| title_full_unstemmed | Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay |
| title_short | Relative assessment of anti-quorum sensing in Piper betle leaves extract via pyoverdin assay |
| title_sort | relative assessment of anti-quorum sensing in piper betle leaves extract via pyoverdin assay |
| topic | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/36009/ http://umpir.ump.edu.my/id/eprint/36009/ http://umpir.ump.edu.my/id/eprint/36009/1/Relative%20assessment%20of%20anti-quorum%20sensing%20in%20Piper%20betle%20leaves%20extract%20via%20pyoverdin%20assay.pdf |