Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time
Pineapple leaves; is one of lignocellulosic materials that composed of about 80% of carbohydrate containing lignin, cellulose and hemicellulose This waste still retains a considerable amount of soluble sugars that can be transformed into valuable fermentable sugars. This research was conducted to st...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Institute of Physics Publishing
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/35975/ http://umpir.ump.edu.my/id/eprint/35975/1/Enzymatic%20hydrolysis%20of%20lignocellulosic%20biomass%20from%20pineapple%20leaves%20by%20using%20endo-1%2C4-xylanase_Effect%20of%20pH%2C%20temperature%2C%20enzyme%20loading.pdf |
| _version_ | 1848824919883776000 |
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| author | N. A. S. M., Rosdee N., Masngut S. M., Shaarani S., Jamek M. S. M., Sueb |
| author_facet | N. A. S. M., Rosdee N., Masngut S. M., Shaarani S., Jamek M. S. M., Sueb |
| author_sort | N. A. S. M., Rosdee |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Pineapple leaves; is one of lignocellulosic materials that composed of about 80% of carbohydrate containing lignin, cellulose and hemicellulose This waste still retains a considerable amount of soluble sugars that can be transformed into valuable fermentable sugars. This research was conducted to study the effect of different pH, temperature, enzyme loading and reaction time in order to identify the best working conditions to produce the highest yield of sugar. Prior to enzymatic hydrolysis, the pineapple leaves were subjected to hydrothermal pretreatment in order to obtain the hydrolysate liquid. Enzymatic hydrolysis was then carried out by using endo-1,4-xylanase and the reducing sugar was analyzed by using dinitrosalicylic (DNS) method. The result has shown that the highest reducing sugar concentration of 70.9 mg/L was obtained both at pH 5.5 and 50 °C. In addition, the best enzyme loading and reaction time were recorded at 0.5% (w/v) and 45 min that resulted 60.1 mg/L and 72. 0 mg/L respectively. |
| first_indexed | 2025-11-15T03:20:41Z |
| format | Conference or Workshop Item |
| id | ump-35975 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:20:41Z |
| publishDate | 2020 |
| publisher | Institute of Physics Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-359752022-12-28T04:53:48Z http://umpir.ump.edu.my/id/eprint/35975/ Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time N. A. S. M., Rosdee N., Masngut S. M., Shaarani S., Jamek M. S. M., Sueb Q Science (General) QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Pineapple leaves; is one of lignocellulosic materials that composed of about 80% of carbohydrate containing lignin, cellulose and hemicellulose This waste still retains a considerable amount of soluble sugars that can be transformed into valuable fermentable sugars. This research was conducted to study the effect of different pH, temperature, enzyme loading and reaction time in order to identify the best working conditions to produce the highest yield of sugar. Prior to enzymatic hydrolysis, the pineapple leaves were subjected to hydrothermal pretreatment in order to obtain the hydrolysate liquid. Enzymatic hydrolysis was then carried out by using endo-1,4-xylanase and the reducing sugar was analyzed by using dinitrosalicylic (DNS) method. The result has shown that the highest reducing sugar concentration of 70.9 mg/L was obtained both at pH 5.5 and 50 °C. In addition, the best enzyme loading and reaction time were recorded at 0.5% (w/v) and 45 min that resulted 60.1 mg/L and 72. 0 mg/L respectively. Institute of Physics Publishing 2020-03-04 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/35975/1/Enzymatic%20hydrolysis%20of%20lignocellulosic%20biomass%20from%20pineapple%20leaves%20by%20using%20endo-1%2C4-xylanase_Effect%20of%20pH%2C%20temperature%2C%20enzyme%20loading.pdf N. A. S. M., Rosdee and N., Masngut and S. M., Shaarani and S., Jamek and M. S. M., Sueb (2020) Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-7., 736 (022095). ISSN 1757-8981 (Published) https://doi.org/10.1088/1757-899X/736/2/022095 |
| spellingShingle | Q Science (General) QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology N. A. S. M., Rosdee N., Masngut S. M., Shaarani S., Jamek M. S. M., Sueb Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time |
| title | Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time |
| title_full | Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time |
| title_fullStr | Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time |
| title_full_unstemmed | Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time |
| title_short | Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time |
| title_sort | enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : effect of ph, temperature, enzyme loading and reaction time |
| topic | Q Science (General) QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/35975/ http://umpir.ump.edu.my/id/eprint/35975/ http://umpir.ump.edu.my/id/eprint/35975/1/Enzymatic%20hydrolysis%20of%20lignocellulosic%20biomass%20from%20pineapple%20leaves%20by%20using%20endo-1%2C4-xylanase_Effect%20of%20pH%2C%20temperature%2C%20enzyme%20loading.pdf |