Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis
A recent upsurge in ocular infections is a pointer towards an enhanced prevalence of ophthalmic disorders, posing challenges for researchers globally. The caveats of conventional therapeutics demand a specifically designed Ocular Drug Delivery System (ODDS) and hence the primary objective of the pre...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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Elsevier Ltd
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/35550/ http://umpir.ump.edu.my/id/eprint/35550/1/Doe%20guided%20chitosan%20based%20nano-ophthalmic%20preparation%20against%20fungal%20keratitis.pdf |
| _version_ | 1848824807538294784 |
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| author | Hassan, Nazia Mirza, Mohd Aamir Aslam, Mohammed Mahmood, Syed Iqbal, Zeenat |
| author_facet | Hassan, Nazia Mirza, Mohd Aamir Aslam, Mohammed Mahmood, Syed Iqbal, Zeenat |
| author_sort | Hassan, Nazia |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | A recent upsurge in ocular infections is a pointer towards an enhanced prevalence of ophthalmic disorders, posing challenges for researchers globally. The caveats of conventional therapeutics demand a specifically designed Ocular Drug Delivery System (ODDS) and hence the primary objective of the present work is a fabrication of a Design of Expert (DoE) guided Chitosan based Antifungal loaded Nanoparticles (CANs), as a locoregionally effective eye formulation/drops for fungal keratitis therapy. The purported formulation was prepared using High-Pressure Homogenisation technique and was critically characterized on various parameters to check their suitability as an ODDS. The optimized formulation has fruitfully yielded irregularly spherical particles in up to a size of 200 nm and a Poly-dispersity Index (PDI) of less than 0.2 nm. The optimised formulation has further showcased a high mucoadhesion capacity thereby, suggesting the greater retention of CANs on the mucous membrane of an eye with low ocular irritancy as highlighted using HET-CAM (Hen's Egg Chorioallantoic Membrane) test. The in-vitro drug release study across a dialysis membrane has indicated both diffusion as swelling controlled release pattern for an optimized formulation. The ex-vivo corneal permeation study on goat corneal tissues using a Franz-Diffusion cell also has indicated a steady increase in the permeation of drug with time for an optimized formulation. Further, the optimised formulation was found to be non-irritant and ocular safe in ex-vivo transcorneal toxicity studies on goat corneal tissues. In conclusion, the designing of a proposed nanosized formulation, offers a promising step towards the management of external ocular diseases with a positive attributes of high patient compliance, controlled drug delivery, prolonged drug precorneal residence time and enhanced ocular bioavailability. The optimized CANs could be further exploited as a potential ODDS. |
| first_indexed | 2025-11-15T03:18:54Z |
| format | Conference or Workshop Item |
| id | ump-35550 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:18:54Z |
| publishDate | 2020 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-355502022-11-03T03:55:07Z http://umpir.ump.edu.my/id/eprint/35550/ Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis Hassan, Nazia Mirza, Mohd Aamir Aslam, Mohammed Mahmood, Syed Iqbal, Zeenat QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology A recent upsurge in ocular infections is a pointer towards an enhanced prevalence of ophthalmic disorders, posing challenges for researchers globally. The caveats of conventional therapeutics demand a specifically designed Ocular Drug Delivery System (ODDS) and hence the primary objective of the present work is a fabrication of a Design of Expert (DoE) guided Chitosan based Antifungal loaded Nanoparticles (CANs), as a locoregionally effective eye formulation/drops for fungal keratitis therapy. The purported formulation was prepared using High-Pressure Homogenisation technique and was critically characterized on various parameters to check their suitability as an ODDS. The optimized formulation has fruitfully yielded irregularly spherical particles in up to a size of 200 nm and a Poly-dispersity Index (PDI) of less than 0.2 nm. The optimised formulation has further showcased a high mucoadhesion capacity thereby, suggesting the greater retention of CANs on the mucous membrane of an eye with low ocular irritancy as highlighted using HET-CAM (Hen's Egg Chorioallantoic Membrane) test. The in-vitro drug release study across a dialysis membrane has indicated both diffusion as swelling controlled release pattern for an optimized formulation. The ex-vivo corneal permeation study on goat corneal tissues using a Franz-Diffusion cell also has indicated a steady increase in the permeation of drug with time for an optimized formulation. Further, the optimised formulation was found to be non-irritant and ocular safe in ex-vivo transcorneal toxicity studies on goat corneal tissues. In conclusion, the designing of a proposed nanosized formulation, offers a promising step towards the management of external ocular diseases with a positive attributes of high patient compliance, controlled drug delivery, prolonged drug precorneal residence time and enhanced ocular bioavailability. The optimized CANs could be further exploited as a potential ODDS. Elsevier Ltd 2020 Conference or Workshop Item PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/35550/1/Doe%20guided%20chitosan%20based%20nano-ophthalmic%20preparation%20against%20fungal%20keratitis.pdf Hassan, Nazia and Mirza, Mohd Aamir and Aslam, Mohammed and Mahmood, Syed and Iqbal, Zeenat (2020) Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis. In: Materials Today: Proceedings, 11th Malaysian Technical Universities Conference on Engineering & Technology , 19-22 November 2019 , Bukit Gambang Resort City, Pahang, Malaysia. pp. 19-29., 41 (Part 1). ISSN 2214-7853 (Published) https://doi.org/10.1016/j.matpr.2020.09.823 |
| spellingShingle | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Hassan, Nazia Mirza, Mohd Aamir Aslam, Mohammed Mahmood, Syed Iqbal, Zeenat Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
| title | Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
| title_full | Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
| title_fullStr | Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
| title_full_unstemmed | Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
| title_short | Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
| title_sort | doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
| topic | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/35550/ http://umpir.ump.edu.my/id/eprint/35550/ http://umpir.ump.edu.my/id/eprint/35550/1/Doe%20guided%20chitosan%20based%20nano-ophthalmic%20preparation%20against%20fungal%20keratitis.pdf |