Preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method
Objective: The objective of this research is to prepare Low Molecular Weight Chitosan (LMWC) by the acid hydrolysis method, using dilute hydrochloric acid (2M). LMWC has superior properties compared to the High Molecular Weight Chitosan (HMWC), especially in terms of water solubility, antibacterial...
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Innovare Academics Sciences Pvt. Ltd
2021
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| Online Access: | http://umpir.ump.edu.my/id/eprint/35162/ http://umpir.ump.edu.my/id/eprint/35162/1/Preparation%20and%20characterization%20of%20low%20molecular%20weight%20chitosan%20with%20different%20degrees%20of%20deacetylation%20by%20the%20acid%20hydrolysis%20method.pdf |
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| author | Al-Jbour, Nawzat D. M. D. H., Beg Jolius, Gimbun A. K. M., Moshiul Alam |
| author_facet | Al-Jbour, Nawzat D. M. D. H., Beg Jolius, Gimbun A. K. M., Moshiul Alam |
| author_sort | Al-Jbour, Nawzat D. |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Objective: The objective of this research is to prepare Low Molecular Weight Chitosan (LMWC) by the acid hydrolysis method, using dilute hydrochloric acid (2M). LMWC has superior properties compared to the High Molecular Weight Chitosan (HMWC), especially in terms of water solubility, antibacterial and antifungal properties. These could open new potential applications for LMWC in sectors such as the cosmetics, food, and pharmaceutical industries. Methods: In this work, the acid hydrolysis method was used to produce LMWC with different molecular weights starting from 500 kDa and 93% degree of deacetylations (DDA). The molecular weights of the produced grades were determined by applying Mark-Houwink equation while the %DDA was determined and verified by the use of the 1st derivative UV method and1HNMR method, respectively. The depolymerization reactions were carried out with different time intervals to produce totally deacetylated LMWC of 30 kDa, 15 kDa, and 7.5 kDa. The LMWC was characterized by FTIR, XRD, and DSC to evaluate the functionality, microstructure and thermal properties. Results: The FTIR spectra revealed that there is no significant difference in the main skeletal structure of the LMWC and HMWC. On the other hand, the XRD and DSC results showed that the LMWC of different molecular weights and degrees of deacetylation are of semi-crystalline structure, similar to the HMWC. Conclusion: The obtained results showed that the used acid hydrolysis procedure can produce LMWC grades of desired specifications, yields, and quality which are suitable for use in different applications. |
| first_indexed | 2025-11-15T03:17:15Z |
| format | Article |
| id | ump-35162 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:17:15Z |
| publishDate | 2021 |
| publisher | Innovare Academics Sciences Pvt. Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-351622022-10-31T06:37:23Z http://umpir.ump.edu.my/id/eprint/35162/ Preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method Al-Jbour, Nawzat D. M. D. H., Beg Jolius, Gimbun A. K. M., Moshiul Alam QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Objective: The objective of this research is to prepare Low Molecular Weight Chitosan (LMWC) by the acid hydrolysis method, using dilute hydrochloric acid (2M). LMWC has superior properties compared to the High Molecular Weight Chitosan (HMWC), especially in terms of water solubility, antibacterial and antifungal properties. These could open new potential applications for LMWC in sectors such as the cosmetics, food, and pharmaceutical industries. Methods: In this work, the acid hydrolysis method was used to produce LMWC with different molecular weights starting from 500 kDa and 93% degree of deacetylations (DDA). The molecular weights of the produced grades were determined by applying Mark-Houwink equation while the %DDA was determined and verified by the use of the 1st derivative UV method and1HNMR method, respectively. The depolymerization reactions were carried out with different time intervals to produce totally deacetylated LMWC of 30 kDa, 15 kDa, and 7.5 kDa. The LMWC was characterized by FTIR, XRD, and DSC to evaluate the functionality, microstructure and thermal properties. Results: The FTIR spectra revealed that there is no significant difference in the main skeletal structure of the LMWC and HMWC. On the other hand, the XRD and DSC results showed that the LMWC of different molecular weights and degrees of deacetylation are of semi-crystalline structure, similar to the HMWC. Conclusion: The obtained results showed that the used acid hydrolysis procedure can produce LMWC grades of desired specifications, yields, and quality which are suitable for use in different applications. Innovare Academics Sciences Pvt. Ltd 2021 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/35162/1/Preparation%20and%20characterization%20of%20low%20molecular%20weight%20chitosan%20with%20different%20degrees%20of%20deacetylation%20by%20the%20acid%20hydrolysis%20method.pdf Al-Jbour, Nawzat D. and M. D. H., Beg and Jolius, Gimbun and A. K. M., Moshiul Alam (2021) Preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method. International Journal of Applied Pharmaceutics, 13 (2). pp. 153-164. ISSN 0975-7058. (Published) https://doi.org/10.22159/ijap.2021v13i2.32229 https://doi.org/10.22159/ijap.2021v13i2.32229 |
| spellingShingle | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Al-Jbour, Nawzat D. M. D. H., Beg Jolius, Gimbun A. K. M., Moshiul Alam Preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method |
| title | Preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method |
| title_full | Preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method |
| title_fullStr | Preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method |
| title_full_unstemmed | Preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method |
| title_short | Preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method |
| title_sort | preparation and characterization of low molecular weight chitosan with different degrees of deacetylation by the acid hydrolysis method |
| topic | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/35162/ http://umpir.ump.edu.my/id/eprint/35162/ http://umpir.ump.edu.my/id/eprint/35162/ http://umpir.ump.edu.my/id/eprint/35162/1/Preparation%20and%20characterization%20of%20low%20molecular%20weight%20chitosan%20with%20different%20degrees%20of%20deacetylation%20by%20the%20acid%20hydrolysis%20method.pdf |