Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters
Biofuel is one of the best ways to reduce our dependence on fossil fuels. Ever since commercial biodiesel production began, waste glycerol, the biodiesel byproduct, has gained researchers’ interest, especially its recycling. Here, we explored using glycerol residue (carbon source) as a substrate in...
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| Format: | Article |
| Language: | English |
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CSIR-NIScPR, India
2022
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| Online Access: | http://umpir.ump.edu.my/id/eprint/35107/ http://umpir.ump.edu.my/id/eprint/35107/1/Bioconversion%20of%20glycerol%20waste%20to%20ethanol.pdf |
| _version_ | 1848824692087980032 |
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| author | Reddy, Chilakamarry Chaitanya Mimi Sakinah, A. M. Zularisam, A. W. Khilji, Irshad Ahmad Sirohi, Ranjna Reddy, Venugopal Jayarama |
| author_facet | Reddy, Chilakamarry Chaitanya Mimi Sakinah, A. M. Zularisam, A. W. Khilji, Irshad Ahmad Sirohi, Ranjna Reddy, Venugopal Jayarama |
| author_sort | Reddy, Chilakamarry Chaitanya |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Biofuel is one of the best ways to reduce our dependence on fossil fuels. Ever since commercial biodiesel production began, waste glycerol, the biodiesel byproduct, has gained researchers’ interest, especially its recycling. Here, we explored using glycerol residue (carbon source) as a substrate in the fermentation process for ethanol production by Escherichia coli K12 in anaerobic conditions. The factors affecting the ethanol production was optimised by response surface methodology (RSM). Significant variables that impact the ethanol concentration were pH, temperature and the substrate, with a statistically significant effect (P <0.05) on ethanol formation. The significant factor was analyzed by the Box-Behnken design. The optimum conditions for bioethanol formation using glycerol as substrate was obtained at pH 7 and temperature 37°C. The ethanol productivity was 0.77 g/L/h. The ethanol concentration of 9.2 g/L achieved from glycerol residue was close to the theoretical value with the fermentation achieved at optimised terms. |
| first_indexed | 2025-11-15T03:17:04Z |
| format | Article |
| id | ump-35107 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:17:04Z |
| publishDate | 2022 |
| publisher | CSIR-NIScPR, India |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-351072022-09-06T09:03:12Z http://umpir.ump.edu.my/id/eprint/35107/ Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters Reddy, Chilakamarry Chaitanya Mimi Sakinah, A. M. Zularisam, A. W. Khilji, Irshad Ahmad Sirohi, Ranjna Reddy, Venugopal Jayarama TP Chemical technology Biofuel is one of the best ways to reduce our dependence on fossil fuels. Ever since commercial biodiesel production began, waste glycerol, the biodiesel byproduct, has gained researchers’ interest, especially its recycling. Here, we explored using glycerol residue (carbon source) as a substrate in the fermentation process for ethanol production by Escherichia coli K12 in anaerobic conditions. The factors affecting the ethanol production was optimised by response surface methodology (RSM). Significant variables that impact the ethanol concentration were pH, temperature and the substrate, with a statistically significant effect (P <0.05) on ethanol formation. The significant factor was analyzed by the Box-Behnken design. The optimum conditions for bioethanol formation using glycerol as substrate was obtained at pH 7 and temperature 37°C. The ethanol productivity was 0.77 g/L/h. The ethanol concentration of 9.2 g/L achieved from glycerol residue was close to the theoretical value with the fermentation achieved at optimised terms. CSIR-NIScPR, India 2022 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/35107/1/Bioconversion%20of%20glycerol%20waste%20to%20ethanol.pdf Reddy, Chilakamarry Chaitanya and Mimi Sakinah, A. M. and Zularisam, A. W. and Khilji, Irshad Ahmad and Sirohi, Ranjna and Reddy, Venugopal Jayarama (2022) Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters. Indian Journal of Experimental Biology (IJEB), 60 (9). pp. 681-688. (Published) http://op.niscpr.res.in/index.php/IJEB/article/view/65145 http://op.niscpr.res.in/index.php/IJEB/article/view/65145 |
| spellingShingle | TP Chemical technology Reddy, Chilakamarry Chaitanya Mimi Sakinah, A. M. Zularisam, A. W. Khilji, Irshad Ahmad Sirohi, Ranjna Reddy, Venugopal Jayarama Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters |
| title | Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters |
| title_full | Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters |
| title_fullStr | Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters |
| title_full_unstemmed | Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters |
| title_short | Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters |
| title_sort | bioconversion of glycerol waste to ethanol by escherichia coli and optimisation of process parameters |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/35107/ http://umpir.ump.edu.my/id/eprint/35107/ http://umpir.ump.edu.my/id/eprint/35107/ http://umpir.ump.edu.my/id/eprint/35107/1/Bioconversion%20of%20glycerol%20waste%20to%20ethanol.pdf |