ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle
The gut mycobiome exhibits major influence on the gastrointestinal health and disease but received less attention due to low abundance. This study characterizes the fungal community and compares the microbial diversity between indoor and outdoor cats. Genomic DNA was extracted and sequenced by targe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Springer
2022
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| Online Access: | http://umpir.ump.edu.my/id/eprint/34570/ http://umpir.ump.edu.my/id/eprint/34570/1/ITS1%20amplicon%20sequencing%20of%20feline%20gut%20mycobiome%20.pdf http://umpir.ump.edu.my/id/eprint/34570/2/ITS1%20amplicon%20sequencing%20of%20feline%20gut%20mycobiome_FULL.pdf |
| _version_ | 1848824541434871808 |
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| author | Tay, Darren Dean Siew, Shing Wei Shamrulazhar, Shamzir Kamal Mohd Najib, Razali Hajar Fauzan, Ahmad |
| author_facet | Tay, Darren Dean Siew, Shing Wei Shamrulazhar, Shamzir Kamal Mohd Najib, Razali Hajar Fauzan, Ahmad |
| author_sort | Tay, Darren Dean |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The gut mycobiome exhibits major influence on the gastrointestinal health and disease but received less attention due to low abundance. This study characterizes the fungal community and compares the microbial diversity between indoor and outdoor cats. Genomic DNA was extracted and sequenced by targeting the Internal Transcribed Spacer 1 (ITS1) region using Flongle flow cell on MinION™ sequencing platform. Results show the phylum Ascomycota and genus Peniophorella were numerous in indoor cats, whereas the Basidiomycota and Pichia were abundant in outdoor cats. Peniophorella formed the core mycobiome in both feline populations. Furthermore, alpha (p value = 0.0207) and beta diversities (p value = 0.009) results showed significant differences between the two groups. Overall, indoor cats have greater amounts of Peniophorella, whereas outdoor cats have higher Trichosporon and unclassified Sordariaceae. The study also suggests that keeping a cat indoors or left as a stray will affect their respective gut mycobiome. |
| first_indexed | 2025-11-15T03:14:40Z |
| format | Article |
| id | ump-34570 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T03:14:40Z |
| publishDate | 2022 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-345702022-07-01T03:23:51Z http://umpir.ump.edu.my/id/eprint/34570/ ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle Tay, Darren Dean Siew, Shing Wei Shamrulazhar, Shamzir Kamal Mohd Najib, Razali Hajar Fauzan, Ahmad QP Physiology TP Chemical technology The gut mycobiome exhibits major influence on the gastrointestinal health and disease but received less attention due to low abundance. This study characterizes the fungal community and compares the microbial diversity between indoor and outdoor cats. Genomic DNA was extracted and sequenced by targeting the Internal Transcribed Spacer 1 (ITS1) region using Flongle flow cell on MinION™ sequencing platform. Results show the phylum Ascomycota and genus Peniophorella were numerous in indoor cats, whereas the Basidiomycota and Pichia were abundant in outdoor cats. Peniophorella formed the core mycobiome in both feline populations. Furthermore, alpha (p value = 0.0207) and beta diversities (p value = 0.009) results showed significant differences between the two groups. Overall, indoor cats have greater amounts of Peniophorella, whereas outdoor cats have higher Trichosporon and unclassified Sordariaceae. The study also suggests that keeping a cat indoors or left as a stray will affect their respective gut mycobiome. Springer 2022 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/34570/1/ITS1%20amplicon%20sequencing%20of%20feline%20gut%20mycobiome%20.pdf pdf en http://umpir.ump.edu.my/id/eprint/34570/2/ITS1%20amplicon%20sequencing%20of%20feline%20gut%20mycobiome_FULL.pdf Tay, Darren Dean and Siew, Shing Wei and Shamrulazhar, Shamzir Kamal and Mohd Najib, Razali and Hajar Fauzan, Ahmad (2022) ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle. Archives of Microbiology, 204 (6). pp. 1-11. ISSN 0302-8933. (Published) https://doi.org/10.1007/s00203-022-02929-3 https://doi.org/10.1007/s00203-022-02929-3 |
| spellingShingle | QP Physiology TP Chemical technology Tay, Darren Dean Siew, Shing Wei Shamrulazhar, Shamzir Kamal Mohd Najib, Razali Hajar Fauzan, Ahmad ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle |
| title | ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle |
| title_full | ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle |
| title_fullStr | ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle |
| title_full_unstemmed | ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle |
| title_short | ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle |
| title_sort | its1 amplicon sequencing of feline gut mycobiome of malaysian local breeds using nanopore flongle |
| topic | QP Physiology TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/34570/ http://umpir.ump.edu.my/id/eprint/34570/ http://umpir.ump.edu.my/id/eprint/34570/ http://umpir.ump.edu.my/id/eprint/34570/1/ITS1%20amplicon%20sequencing%20of%20feline%20gut%20mycobiome%20.pdf http://umpir.ump.edu.my/id/eprint/34570/2/ITS1%20amplicon%20sequencing%20of%20feline%20gut%20mycobiome_FULL.pdf |