Morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems

As hypercholesterolemia is a primary risk factor for coronary artery disease and stroke, there is now an increasing demand for cholesterol-lowering drugs. Statins are a group of extremely successful drugs that lower the cholesterol level in the blood. Natural statins are produced by fermentation usi...

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Main Authors: Seydametova, Emine, Norazwina, Zainol
Format: Article
Language:English
Published: Elsevier Ltd 2021
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/33384/
http://umpir.ump.edu.my/id/eprint/33384/1/Morphological%2C%20physiological%2C%20biochemical%20and%20molecular%20characterization.pdf
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author Seydametova, Emine
Norazwina, Zainol
author_facet Seydametova, Emine
Norazwina, Zainol
author_sort Seydametova, Emine
building UMP Institutional Repository
collection Online Access
description As hypercholesterolemia is a primary risk factor for coronary artery disease and stroke, there is now an increasing demand for cholesterol-lowering drugs. Statins are a group of extremely successful drugs that lower the cholesterol level in the blood. Natural statins are produced by fermentation using different species of microorganisms. The overall aim of the present study was to identify statin-producing microfungi, which were isolated from different types of little-explored mangrove and oil palm plantation soils. Isolated fungal cultures were characterized on the basis of morphological, physiological, biochemical, and molecular features. Morphological variability was detected amongst the fungal isolates in regard to colony morphology, conidiophores structures, and conidia coloration. Based on their physiological properties and enzyme assays, rapid differentiation of statin-producing isolates was achieved. Further molecular characterization allowed reliable identification of the selected Penicillium microfungi up to the species level. The identified Penicillium cintrinum ESF2M, Penicillium brefeldianum ESF21P, and Penicillium janthinellum ESF26P strains have a scientific interest as novel wild-type producers of natural statins.
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spelling ump-333842022-03-22T03:14:27Z http://umpir.ump.edu.my/id/eprint/33384/ Morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems Seydametova, Emine Norazwina, Zainol QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology As hypercholesterolemia is a primary risk factor for coronary artery disease and stroke, there is now an increasing demand for cholesterol-lowering drugs. Statins are a group of extremely successful drugs that lower the cholesterol level in the blood. Natural statins are produced by fermentation using different species of microorganisms. The overall aim of the present study was to identify statin-producing microfungi, which were isolated from different types of little-explored mangrove and oil palm plantation soils. Isolated fungal cultures were characterized on the basis of morphological, physiological, biochemical, and molecular features. Morphological variability was detected amongst the fungal isolates in regard to colony morphology, conidiophores structures, and conidia coloration. Based on their physiological properties and enzyme assays, rapid differentiation of statin-producing isolates was achieved. Further molecular characterization allowed reliable identification of the selected Penicillium microfungi up to the species level. The identified Penicillium cintrinum ESF2M, Penicillium brefeldianum ESF21P, and Penicillium janthinellum ESF26P strains have a scientific interest as novel wild-type producers of natural statins. Elsevier Ltd 2021-12 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/33384/1/Morphological%2C%20physiological%2C%20biochemical%20and%20molecular%20characterization.pdf Seydametova, Emine and Norazwina, Zainol (2021) Morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems. Current Research in Microbial Sciences, 2 (100044). pp. 1-8. ISSN 2666-5174. (Published) https://doi.org/10.1016/j.crmicr.2021.100044 https://doi.org/10.1016/j.crmicr.2021.100044
spellingShingle QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
Seydametova, Emine
Norazwina, Zainol
Morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems
title Morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems
title_full Morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems
title_fullStr Morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems
title_full_unstemmed Morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems
title_short Morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems
title_sort morphological, physiological, biochemical and molecular characterization of statin-producing penicillium microfungi isolated from little-explored tropical ecosystems
topic QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/33384/
http://umpir.ump.edu.my/id/eprint/33384/
http://umpir.ump.edu.my/id/eprint/33384/
http://umpir.ump.edu.my/id/eprint/33384/1/Morphological%2C%20physiological%2C%20biochemical%20and%20molecular%20characterization.pdf