Induction of apoptosis and regulation ofMicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells
(2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic eff...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021
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| Online Access: | http://umpir.ump.edu.my/id/eprint/32769/ http://umpir.ump.edu.my/id/eprint/32769/1/Induction%20of%20apoptosis%20and%20regulation%20ofMicroRNA%20expression%20by%20%282E%2C6E%29-2%2C6-bis-%284-hydroxy-3-methoxybenzylidene%29-%20cyclohexanone%20%28BHMC%29%20treatment.pdf |
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| author | Yeap, S. K. Ali, N. M. Akhtar, M. N. Razak, N. A. Chong, Z. X. Ho, W. Y. Boo, L. Zareen, S. Kurniawan, T. A. Avtar, R. Ng, S. Y. L. Ong, A. H. K. |
| author_facet | Yeap, S. K. Ali, N. M. Akhtar, M. N. Razak, N. A. Chong, Z. X. Ho, W. Y. Boo, L. Zareen, S. Kurniawan, T. A. Avtar, R. Ng, S. Y. L. Ong, A. H. K. |
| author_sort | Yeap, S. K. |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes. |
| first_indexed | 2025-11-15T03:07:45Z |
| format | Article |
| id | ump-32769 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:07:45Z |
| publishDate | 2021 |
| publisher | MDPI AG |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-327692022-03-08T04:16:12Z http://umpir.ump.edu.my/id/eprint/32769/ Induction of apoptosis and regulation ofMicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells Yeap, S. K. Ali, N. M. Akhtar, M. N. Razak, N. A. Chong, Z. X. Ho, W. Y. Boo, L. Zareen, S. Kurniawan, T. A. Avtar, R. Ng, S. Y. L. Ong, A. H. K. RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) T Technology (General) (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes. MDPI AG 2021-02-26 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/32769/1/Induction%20of%20apoptosis%20and%20regulation%20ofMicroRNA%20expression%20by%20%282E%2C6E%29-2%2C6-bis-%284-hydroxy-3-methoxybenzylidene%29-%20cyclohexanone%20%28BHMC%29%20treatment.pdf Yeap, S. K. and Ali, N. M. and Akhtar, M. N. and Razak, N. A. and Chong, Z. X. and Ho, W. Y. and Boo, L. and Zareen, S. and Kurniawan, T. A. and Avtar, R. and Ng, S. Y. L. and Ong, A. H. K. (2021) Induction of apoptosis and regulation ofMicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells. Molecules, 26 (5). pp. 1-15. ISSN 1420-3049. (Published) https://doi.org/10.3390/molecules26051277 https://doi.org/10.3390/molecules26051277 |
| spellingShingle | RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) T Technology (General) Yeap, S. K. Ali, N. M. Akhtar, M. N. Razak, N. A. Chong, Z. X. Ho, W. Y. Boo, L. Zareen, S. Kurniawan, T. A. Avtar, R. Ng, S. Y. L. Ong, A. H. K. Induction of apoptosis and regulation ofMicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title | Induction of apoptosis and regulation ofMicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_full | Induction of apoptosis and regulation ofMicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_fullStr | Induction of apoptosis and regulation ofMicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_full_unstemmed | Induction of apoptosis and regulation ofMicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_short | Induction of apoptosis and regulation ofMicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_sort | induction of apoptosis and regulation ofmicrorna expression by (2e,6e)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)- cyclohexanone (bhmc) treatment on mcf-7 breast cancer cells |
| topic | RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/32769/ http://umpir.ump.edu.my/id/eprint/32769/ http://umpir.ump.edu.my/id/eprint/32769/ http://umpir.ump.edu.my/id/eprint/32769/1/Induction%20of%20apoptosis%20and%20regulation%20ofMicroRNA%20expression%20by%20%282E%2C6E%29-2%2C6-bis-%284-hydroxy-3-methoxybenzylidene%29-%20cyclohexanone%20%28BHMC%29%20treatment.pdf |