Discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study
Seaweeds are a marine source rich in potentially bioactive components, and therefore have attracted attention since the middle of the twentieth century. Accurate and objective assessment of the intake of seaweeds to study their health effects is hampered by a lack of validated intake biomarkers. In...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/32763/ http://umpir.ump.edu.my/id/eprint/32763/1/Discovery%20of%20urinary%20biomarkers%20of%20seaweed%20intake%20using%20untargeted%20lc%E2%80%93ms%20metabolomics.pdf |
| _version_ | 1848824103989936128 |
|---|---|
| author | Xi, M. Dragsted, L. O. Tullin, M. Ernst, M. Nazikussabah, Zaharudin Barbera, G. L. |
| author_facet | Xi, M. Dragsted, L. O. Tullin, M. Ernst, M. Nazikussabah, Zaharudin Barbera, G. L. |
| author_sort | Xi, M. |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Seaweeds are a marine source rich in potentially bioactive components, and therefore have attracted attention since the middle of the twentieth century. Accurate and objective assessment of the intake of seaweeds to study their health effects is hampered by a lack of validated intake biomarkers. In this three-armed, randomized, cross-over study, an untargeted metabolomics approach was applied for discovering novel intake biomarkers. Twenty healthy participants (9 men and 11 women) were provided each of three test meals in a randomized order: 5 g of Laminaria digitate (LD), 5 g of Undaria pinnatifida (UP), or a control meal with energy-adjusted pea protein. Four urine samples and a 24 h pooled urine were collected along with blood samples at seven time-points. All samples were profiled by LC-ESI-QTOF-MS and the data were analyzed by univariate analysis and excretion kinetics to select putative intake biomarkers. In total, four intake biomarkers were selected from urine samples. They were identified as hydroxyl-dihydrocoumarin at Level III, loliolid glucuronide at level I, and isololiolid glucuronide at level II, while the last one remains unknown. Further identification and validation of these biomarkers by a cross-sectional study is essential to assess their specificity and robustness. |
| first_indexed | 2025-11-15T03:07:43Z |
| format | Article |
| id | ump-32763 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:07:43Z |
| publishDate | 2021 |
| publisher | MDPI AG |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-327632022-02-23T04:13:51Z http://umpir.ump.edu.my/id/eprint/32763/ Discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study Xi, M. Dragsted, L. O. Tullin, M. Ernst, M. Nazikussabah, Zaharudin Barbera, G. L. T Technology (General) TP Chemical technology Seaweeds are a marine source rich in potentially bioactive components, and therefore have attracted attention since the middle of the twentieth century. Accurate and objective assessment of the intake of seaweeds to study their health effects is hampered by a lack of validated intake biomarkers. In this three-armed, randomized, cross-over study, an untargeted metabolomics approach was applied for discovering novel intake biomarkers. Twenty healthy participants (9 men and 11 women) were provided each of three test meals in a randomized order: 5 g of Laminaria digitate (LD), 5 g of Undaria pinnatifida (UP), or a control meal with energy-adjusted pea protein. Four urine samples and a 24 h pooled urine were collected along with blood samples at seven time-points. All samples were profiled by LC-ESI-QTOF-MS and the data were analyzed by univariate analysis and excretion kinetics to select putative intake biomarkers. In total, four intake biomarkers were selected from urine samples. They were identified as hydroxyl-dihydrocoumarin at Level III, loliolid glucuronide at level I, and isololiolid glucuronide at level II, while the last one remains unknown. Further identification and validation of these biomarkers by a cross-sectional study is essential to assess their specificity and robustness. MDPI AG 2021-01 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/32763/1/Discovery%20of%20urinary%20biomarkers%20of%20seaweed%20intake%20using%20untargeted%20lc%E2%80%93ms%20metabolomics.pdf Xi, M. and Dragsted, L. O. and Tullin, M. and Ernst, M. and Nazikussabah, Zaharudin and Barbera, G. L. (2021) Discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study. Metabolites, 11 (1). pp. 1-15. ISSN 2218-1989. (Published) https://doi.org/10.3390/metabo11010011 https://doi.org/10.3390/metabo11010011 |
| spellingShingle | T Technology (General) TP Chemical technology Xi, M. Dragsted, L. O. Tullin, M. Ernst, M. Nazikussabah, Zaharudin Barbera, G. L. Discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study |
| title | Discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study |
| title_full | Discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study |
| title_fullStr | Discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study |
| title_full_unstemmed | Discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study |
| title_short | Discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study |
| title_sort | discovery of urinary biomarkers of seaweed intake using untargeted lc–ms metabolomics in a three-way cross-over human study |
| topic | T Technology (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/32763/ http://umpir.ump.edu.my/id/eprint/32763/ http://umpir.ump.edu.my/id/eprint/32763/ http://umpir.ump.edu.my/id/eprint/32763/1/Discovery%20of%20urinary%20biomarkers%20of%20seaweed%20intake%20using%20untargeted%20lc%E2%80%93ms%20metabolomics.pdf |