The putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (NSP3 HVD) in evolution, viability and re-emergence

Alphavirus non-structural proteins 1–4 (nsP1, nsP2, nsP3, and nsP4) are known to be crucial for alphavirus RNA replication and translation. To date, nsP3 has been demonstrated to mediate many virus–host protein–protein interactions in several fundamental alphavirus mechanisms, particularly during th...

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Main Authors: Nurshariza, Abdullah, Nafees, Ahemad, Aliazis, Konstantinos, Khairat, Jasmine Elanie, Lee, Tong Chuan, Siti Aisyah, Abdul Ahmad, Nur Amelia Azreen, Adnan, Nur Omar, Macha, Sharifah, Syed Hassan
Format: Article
Language:English
Published: MDPI 2021
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/31569/
http://umpir.ump.edu.my/id/eprint/31569/1/viruses-13-01021-v3.pdf
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author Nurshariza, Abdullah
Nafees, Ahemad
Aliazis, Konstantinos
Khairat, Jasmine Elanie
Lee, Tong Chuan
Siti Aisyah, Abdul Ahmad
Nur Amelia Azreen, Adnan
Nur Omar, Macha
Sharifah, Syed Hassan
author_facet Nurshariza, Abdullah
Nafees, Ahemad
Aliazis, Konstantinos
Khairat, Jasmine Elanie
Lee, Tong Chuan
Siti Aisyah, Abdul Ahmad
Nur Amelia Azreen, Adnan
Nur Omar, Macha
Sharifah, Syed Hassan
author_sort Nurshariza, Abdullah
building UMP Institutional Repository
collection Online Access
description Alphavirus non-structural proteins 1–4 (nsP1, nsP2, nsP3, and nsP4) are known to be crucial for alphavirus RNA replication and translation. To date, nsP3 has been demonstrated to mediate many virus–host protein–protein interactions in several fundamental alphavirus mechanisms, particularly during the early stages of replication. However, the molecular pathways and proteins networks underlying these mechanisms remain poorly described. This is due to the low genetic sequence homology of the nsP3 protein among the alphavirus species, especially at its 30 C-terminal domain, the hypervariable domain (HVD). Moreover, the nsP3 HVD is almost or completely intrinsically disordered and has a poor ability to form secondary structures. Evolution in the nsP3 HVD region allows the alphavirus to adapt to vertebrate and insect hosts. This review focuses on the putative roles and functions of indel, repetition, and duplication events that have occurred in the alphavirus nsP3 HVD, including characterization of the differences and their implications for specificity in the context of virus–host interactions in fundamental alphavirus mechanisms, which have thus directly facilitated the evolution, adaptation, viability, and re-emergence of these viruses.
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spelling ump-315692021-06-28T00:54:19Z http://umpir.ump.edu.my/id/eprint/31569/ The putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (NSP3 HVD) in evolution, viability and re-emergence Nurshariza, Abdullah Nafees, Ahemad Aliazis, Konstantinos Khairat, Jasmine Elanie Lee, Tong Chuan Siti Aisyah, Abdul Ahmad Nur Amelia Azreen, Adnan Nur Omar, Macha Sharifah, Syed Hassan QR Microbiology QR355 Virology Alphavirus non-structural proteins 1–4 (nsP1, nsP2, nsP3, and nsP4) are known to be crucial for alphavirus RNA replication and translation. To date, nsP3 has been demonstrated to mediate many virus–host protein–protein interactions in several fundamental alphavirus mechanisms, particularly during the early stages of replication. However, the molecular pathways and proteins networks underlying these mechanisms remain poorly described. This is due to the low genetic sequence homology of the nsP3 protein among the alphavirus species, especially at its 30 C-terminal domain, the hypervariable domain (HVD). Moreover, the nsP3 HVD is almost or completely intrinsically disordered and has a poor ability to form secondary structures. Evolution in the nsP3 HVD region allows the alphavirus to adapt to vertebrate and insect hosts. This review focuses on the putative roles and functions of indel, repetition, and duplication events that have occurred in the alphavirus nsP3 HVD, including characterization of the differences and their implications for specificity in the context of virus–host interactions in fundamental alphavirus mechanisms, which have thus directly facilitated the evolution, adaptation, viability, and re-emergence of these viruses. MDPI 2021-05-28 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/31569/1/viruses-13-01021-v3.pdf Nurshariza, Abdullah and Nafees, Ahemad and Aliazis, Konstantinos and Khairat, Jasmine Elanie and Lee, Tong Chuan and Siti Aisyah, Abdul Ahmad and Nur Amelia Azreen, Adnan and Nur Omar, Macha and Sharifah, Syed Hassan (2021) The putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (NSP3 HVD) in evolution, viability and re-emergence. VIruses, 13 (6). p. 1021. (Published) https://doi.org/10.3390/v13061021
spellingShingle QR Microbiology
QR355 Virology
Nurshariza, Abdullah
Nafees, Ahemad
Aliazis, Konstantinos
Khairat, Jasmine Elanie
Lee, Tong Chuan
Siti Aisyah, Abdul Ahmad
Nur Amelia Azreen, Adnan
Nur Omar, Macha
Sharifah, Syed Hassan
The putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (NSP3 HVD) in evolution, viability and re-emergence
title The putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (NSP3 HVD) in evolution, viability and re-emergence
title_full The putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (NSP3 HVD) in evolution, viability and re-emergence
title_fullStr The putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (NSP3 HVD) in evolution, viability and re-emergence
title_full_unstemmed The putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (NSP3 HVD) in evolution, viability and re-emergence
title_short The putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (NSP3 HVD) in evolution, viability and re-emergence
title_sort putative roles and functions of indel, repetition and duplication events in alphavirus non-structural protein 3 hypervariable domain (nsp3 hvd) in evolution, viability and re-emergence
topic QR Microbiology
QR355 Virology
url http://umpir.ump.edu.my/id/eprint/31569/
http://umpir.ump.edu.my/id/eprint/31569/
http://umpir.ump.edu.my/id/eprint/31569/1/viruses-13-01021-v3.pdf