Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane
Cyclodextrin glucanotransferase (CGTase) is an enzyme that convert starch into cyclodextrin (CD) by transglycosylation reaction. The CD has been used in various industries due to the unique characteristics. However, the production of CD is usually restricted due to the instability of the enzyme whic...
| Main Authors: | , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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IOP Publishing
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/31169/ http://umpir.ump.edu.my/id/eprint/31169/1/Optimization%20of%20reaction%20conditions%20for%20the%20production%20of%20cyclodextrin.pdf |
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| author | S., Suhaimi Rohaida, Che Man Natassha, Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman |
| author_facet | S., Suhaimi Rohaida, Che Man Natassha, Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman |
| author_sort | S., Suhaimi |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Cyclodextrin glucanotransferase (CGTase) is an enzyme that convert starch into cyclodextrin (CD) by transglycosylation reaction. The CD has been used in various industries due to the unique characteristics. However, the production of CD is usually restricted due to the instability of the enzyme which easily denatured during the reaction process. Thus, enzyme immobilization technique was applied and optimization of reaction conditions was conducted to enhance the amount of CD production. In this study, CGTase was immobilized on polyvinylidiene difluoride (PVDF) hollow fiber membrane by adsorption. The optimization of the reaction conditions using response surface methodology (RSM) on the production of CD was studied. Under optimized conditions (2.8% w/v of soluble potato starch concentration, 45.2°C of reaction temperature and pH 5.6), the production of CD was 5.65 mg/mL, about 2-fold compared to the value before optimization process. Therefore, the immobilized CGTase on hollow fiber membrane proved to be valuable for the enhancement of CD production. |
| first_indexed | 2025-11-15T03:01:20Z |
| format | Conference or Workshop Item |
| id | ump-31169 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:01:20Z |
| publishDate | 2020 |
| publisher | IOP Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-311692021-04-28T04:10:08Z http://umpir.ump.edu.my/id/eprint/31169/ Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane S., Suhaimi Rohaida, Che Man Natassha, Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman TP Chemical technology Cyclodextrin glucanotransferase (CGTase) is an enzyme that convert starch into cyclodextrin (CD) by transglycosylation reaction. The CD has been used in various industries due to the unique characteristics. However, the production of CD is usually restricted due to the instability of the enzyme which easily denatured during the reaction process. Thus, enzyme immobilization technique was applied and optimization of reaction conditions was conducted to enhance the amount of CD production. In this study, CGTase was immobilized on polyvinylidiene difluoride (PVDF) hollow fiber membrane by adsorption. The optimization of the reaction conditions using response surface methodology (RSM) on the production of CD was studied. Under optimized conditions (2.8% w/v of soluble potato starch concentration, 45.2°C of reaction temperature and pH 5.6), the production of CD was 5.65 mg/mL, about 2-fold compared to the value before optimization process. Therefore, the immobilized CGTase on hollow fiber membrane proved to be valuable for the enhancement of CD production. IOP Publishing 2020-03-04 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/31169/1/Optimization%20of%20reaction%20conditions%20for%20the%20production%20of%20cyclodextrin.pdf S., Suhaimi and Rohaida, Che Man and Natassha, Jamil and Zatul Iffah, Mohd Arshad and Shalyda, Md Shaarani and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman (2020) Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-8., 736 (042005). ISSN 1757-8981 (Print), 1757-899X (Online) (Published) https://doi.org/10.1088/1757-899X/736/4/042005 https://doi.org/10.1088/1757-899X/736/4/042005 |
| spellingShingle | TP Chemical technology S., Suhaimi Rohaida, Che Man Natassha, Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane |
| title | Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane |
| title_full | Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane |
| title_fullStr | Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane |
| title_full_unstemmed | Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane |
| title_short | Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane |
| title_sort | optimization of reaction conditions for the production of cyclodextrin (cd) using cyclodextrin glucanotransferase (cgtase) immobilized on hollow fiber membrane |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/31169/ http://umpir.ump.edu.my/id/eprint/31169/ http://umpir.ump.edu.my/id/eprint/31169/ http://umpir.ump.edu.my/id/eprint/31169/1/Optimization%20of%20reaction%20conditions%20for%20the%20production%20of%20cyclodextrin.pdf |